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The role of prostaglandin E2 receptors in the pathogenesis of rheumatoid arthritis
Jennifer M. McCoy, … , Joan R. Wicks, Laurent P. Audoly
Jennifer M. McCoy, … , Joan R. Wicks, Laurent P. Audoly
Published September 1, 2002
Citation Information: J Clin Invest. 2002;110(5):651-658. https://doi.org/10.1172/JCI15528.
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Article Genetics

The role of prostaglandin E2 receptors in the pathogenesis of rheumatoid arthritis

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Abstract

Research Article

Authors

Jennifer M. McCoy, Joan R. Wicks, Laurent P. Audoly

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Figure 4

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Role of EP4 receptors in local and systemic inflammatory responses. (a) ...
Role of EP4 receptors in local and systemic inflammatory responses. (a) Detection of EP4 receptor mRNA by RT-PCR in diseased EP4+/+ liver. (b) Total RNA was extracted from EP4+/+ and EP4–/– normal and diseased livers, followed by IL-1β/GAPDH determinations as described in Methods. EP4+/+, 36.4 ± 6.7 (n = 4); and EP4–/–, 34.9 ± 8.2 (n = 4), in arbitrary units (IL-1β pg RNA/GAPDH pg RNA). In contrast, levels were significantly different in diseased liver samples: EP4+/+, 57.4 ± 4.4 (n = 4) and EP4–/–, 20.6 ± 2.7 (n = 4). *P < 0.05 by two-way ANOVA. (c) Detection of EP4 receptor mRNA by RT-PCR from diseased wild-type peritoneal macrophages. (d) Determination of IL-6 (ng/ml) secreted from peritoneal macrophages isolated from diseased EP4+/+ and EP4–/– mice. Media only: EP4+/+, 8.6 ± 0.1 (n = 3); EP4–/–, 5.4 ± 0.1 (n = 3). LPS (1 μg/ml): EP4+/+, 12.9 ± 0.5 (n = 3); EP4–/–, 13.5 ± 0.7 (n = 3). *P < 0.05, by two-way ANOVA. In b and d, black bars are results from EP4+/+ animals and white bars are results from EP4–/– animals. OD β hex, β-hexasiminadase activity.

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