Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids
Florian P. Limbourg, … , Michael A. Moskowitz, James K. Liao
Florian P. Limbourg, … , Michael A. Moskowitz, James K. Liao
Published December 1, 2002
Citation Information: J Clin Invest. 2002;110(11):1729-1738. https://doi.org/10.1172/JCI15481.
View: Text | PDF | Erratum
Article Vascular biology

Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids

Abstract

Research Article

Authors

Florian P. Limbourg, Zhihong Huang, Jean-Christophe Plumier, Tommaso Simoncini, Masayuki Fujioka, Jan Tuckermann, Günther Schütz, Michael A. Moskowitz, James K. Liao

×

Figure 5

Options: View larger image (or click on image) Download as PowerPoint
Nontranscriptional activation of eNOS by high-dose corticosteroids. (a) ...
Nontranscriptional activation of eNOS by high-dose corticosteroids. (a) Shown are cGMP levels in human ECs after dexamethasone treatment. The upper panel shows cGMP levels in HUVECs stimulated with S-nitrosoglutathione (GSNO, 300 μM) or dexamethasone (100 nM) with or without LY294002 (LY, 30 μM) or L-NAME (1 mM, n = 4). The lower panel shows cGMP levels in HAECs stimulated with dexamethasone with or without LY294002 (n = 4). Ctrl, control. *P < 0.05 vs. control. GSNO, S-nitrosoglutathione; LY, LY294002. (b) Effects of 100 nM of dexamethasone, prednisolone, hydrocortisone, or triamcinolone acetonide on eNOS activity in HUVECs (n = 2). Pred, prednisolone; HC, hydrocortisone; TA, triamcinolone acetonide. (c) Dose–response effects of dexamethasone on eNOS activity (HUVECs, n = 5) and GRE-luciferase activity (bovine aortic endothelial cells transfected with pGRE-luc, n = 6). *P < 0.01 vs. control. (d) Dexamethasone serum levels in mice after intraperitoneal bolus administration of low- and high-dose dexamethasone.