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Citations to this article

The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment.
P Webster, … , L M Chicoine, E Fikrig
P Webster, … , L M Chicoine, E Fikrig
Published May 1, 1998
Citation Information: J Clin Invest. 1998;101(9):1932-1941. https://doi.org/10.1172/JCI1544.
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Research Article

The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment.

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Abstract

The composition of cytoplasmic vacuoles containing the agent of Human Granulocytic Ehrlichiosis (HGE) was studied to investigate how this pathogen exists within infected host cells. Electron microscopy demonstrated that the HGE organism resides in a membrane-bound compartment within HL-60 cells: early forms of the HGE agent have a round reticular appearance while later structures are small and dense. Vacuoles containing HGE bacteria incorporated endocytosed colloidal gold particles, suggesting that they are part of the endocytic pathway. Antibodies directed to the mannose-6-phosphate receptor labeled vacuole membranes. Antibodies to the transferrin receptor and to the lysosomal membrane glycoprotein LAMP 1 did not. Moreover, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine, which normally accumulates in compartments with low pH, was not present inside these vacuoles. These results suggest that vacuoles containing the agent of HGE fail to mature into phagolysosomes. We conclude that the agent of HGE appears to enter and modify part of the endocytic pathway.

Authors

P Webster, J W IJdo, L M Chicoine, E Fikrig

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Total citations by year

Year: 2023 2022 2019 2018 2017 2016 2015 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 Total
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