Analysis of Glut-2 expression. (a) Real-time PCR amplification. mRNA was isolated from Igf1r^Δlox/–, Igf1r^lox/–, and Igf1r^Δlox/+ mice (n = 3 each; equal amounts were pooled). Amplification was performed using a one-step RT-PCR reaction. The amount of RNA in the reaction was normalized using β-actin RNA as a control. *P < 0.05 by ANOVA. (b) Immunohistochemistry with anti-GLUT-2 antiserum. Representative sections of Igf1r^Δlox/+ and wild-type mice are shown.