Vitamin E suppresses CD95L mRNA levels. (a) Analysis of the vitamin E effect on CD95L mRNA expression levels by RT-PCR. The day 6 T cells were treated without or with vitamin E and then stimulated for 12 hours with α-CD3. The CD95L mRNA expression levels were analyzed by RT-PCR. For controls, IFN-γ and β-actin mRNA levels were examined. (b) Quantification of the CD95L mRNA expression levels by real-time PCR. The expression value of CD95L in unstimulated cells was set as transcription value 1. The expression value of CD95L in α-CD3–stimulated cells is shown as fold difference relative to that of unstimulated cells.