Duarte C. Barral, José S. Ramalho, Ross Anders, Alistair N. Hume, Holly J. Knapton, Tanya Tolmachova, Lucy M. Collinson, David Goulding, Kalwant S. Authi, Miguel C. Seabra
ELISA detection of endogenous vWF. Platelets from ashen homozygous (ash/ash) and heterozygous mice controls (+/ash) (a) and from gunmetal homozygous (gm/gm) and heterozygous mice controls (+/gm) (b) were lysed and the indicated amount of total protein incubated with 100 μl of buffer in a sandwich ELISA (see Methods). Each step corresponds to a 1:2 dilution, and duplicates were made for each point. The results are representative of three independent experiments. The OD450nm value from the blank reaction (buffer only) was subtracted.