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Citations to this article

Islet β cell expression of constitutively active Akt1/PKBα induces striking hypertrophy, hyperplasia, and hyperinsulinemia
Ernesto Bernal-Mizrachi, … , Cris M. Welling, M. Alan Permutt
Ernesto Bernal-Mizrachi, … , Cris M. Welling, M. Alan Permutt
Published December 1, 2001
Citation Information: J Clin Invest. 2001;108(11):1631-1638. https://doi.org/10.1172/JCI13785.
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Article

Islet β cell expression of constitutively active Akt1/PKBα induces striking hypertrophy, hyperplasia, and hyperinsulinemia

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Abstract

The phosphoinositide 3-kinase–Akt/PKB pathway mediates the mitogenic effects various nutrients and growth factors in cultured cells. To study its effects in vivo in pancreatic islet β cells, we created transgenic mice that expressed a constitutively active Akt1/PKBα linked to an Insulin gene promoter. Transgenic mice exhibited a grossly visible increase in islet mass, largely due to proliferation of insulin-containing β cells. Morphometric analysis verified a six-fold increase in β cell mass/pancreas, a two-fold increase in 5-bromo-2′-deoxyuridine incorporation, a four-fold increase in the number of β cells per pancreas area, and a two-fold increase in cell size in transgenic compared with wild-type mice at 5 weeks. At least part of the increase in β cell number may be accounted for by neogenesis, defined by criteria that include β cells proliferating from ductular epithelium, and by a six-fold increase in the number of single and doublet β cells scattered throughout the exocrine pancreas of the transgenic mice. Glucose tolerance was improved, and fasting as well as fed insulin was greater compared with wild-type mice. Glucose-stimulated insulin secretion was maintained in transgenic mice, which were resistant to streptozotocin–induced diabetes. We conclude that activation of the Akt1/PKBα pathway affects islet β cell mass by alteration of size and number.

Authors

Ernesto Bernal-Mizrachi, Wu Wen, Sarah Stahlhut, Cris M. Welling, M. Alan Permutt

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 Total
Citations: 1 6 13 5 10 8 5 7 8 9 7 14 10 12 9 20 14 19 8 5 6 4 4 2 1 207
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2015 (7)

Title and authors Publication Year
Nephrin Contributes to Insulin Secretion and Affects Mammalian Target of Rapamycin Signaling Independently of Insulin Receptor
R Villarreal, A Mitrofanova, D Maiguel, X Morales, J Jeon, F Grahammer, IB Leibiger, J Guzman, A Fachado, TH Yoo, AB Katin, J Gellermann, S Merscher, GW Burke, PO Berggren, J Oh, TB Huber, A Fornoni
Journal of the American Society of Nephrology : JASN 2015
Human umbilical cord matrix-derived stem cells exert trophic effects on  -cell survival in diabetic rats and isolated islets
Y Zhou, Q Hu, F Chen, J Zhang, J Guo, H Wang, J Gu, L Ma, G Ho
Disease models & mechanisms 2015
Cyclin C stimulates β-cell proliferation in rat and human pancreatic β-cells
M Jiménez-Palomares, JF López-Acosta, P Villa-Pérez, JL Moreno-Amador, J Muñoz-Barrera, S Fernández-Luis, B Heras-Pozas, G Perdomo, E Bernal-Mizrachi, I Cózar-Castellano
American journal of physiology. Endocrinology and metabolism 2015
Ginseng Berry Extract Supplementation Improves Age-Related Decline of Insulin Signaling in Mice
E Seo, S Kim, S Lee, BC Oh, HS Jun
Nutrients 2015
On being the right (cell) size
MB Ginzberg, R Kafri, M Kirschner
Science 2015
Prediabetes linked to excess glucagon in transgenic mice with pancreatic active AKT1
TM Albury-Warren, V Pandey, LP Spinel, MM Masternak, DA Altomare
Journal of Endocrinology 2015
Disruption of O-linked N-Acetylglucosamine Signaling Induces ER Stress and β Cell Failure
EU Alejandro, N Bozadjieva, D Kumusoglu, S Abdulhamid, H Levine, L Haataja, S Vadrevu, LS Satin, P Arvan, E Bernal-Mizrachi
Cell Reports 2015

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