Immunofluorescent immunohistochemical analyses of intracoelomic hybrid intestinal grafts detecting lactase, smooth muscle α-actin, tenascin, or laminin 1 expression. Hybrid intestines composed of 14-day fetal intestinal endoderms surrounded by pCEN cells (a–d), EpiS2 cells (e–h), or EpiAS1 cells (i–l) were developed for 13 days in the coelomic cavity of chicken embryos. Immunostaining of lactase (a, e, and i), smooth muscle α-actin (b, f, and j), tenascin (c, g, and k), and laminin 1 (d, h, and l). Magnification in a, c–e, g–i, k, and l: ×200; in b, f, and j: ×100.