Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis
Christine Fritsch, Elzbieta A. Swietlicki, Olivier Lefebvre, Michele Kedinger, Hristo Iordanov, Marc S. Levin, Deborah C. Rubin
Christine Fritsch, Elzbieta A. Swietlicki, Olivier Lefebvre, Michele Kedinger, Hristo Iordanov, Marc S. Levin, Deborah C. Rubin
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Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis

Abstract

Research Article

Authors

Christine Fritsch, Elzbieta A. Swietlicki, Olivier Lefebvre, Michele Kedinger, Hristo Iordanov, Marc S. Levin, Deborah C. Rubin

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Figure 1

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Western blot analysis of epimorphin/syntaxin 2 expression in transfected...
Western blot analysis of epimorphin/syntaxin 2 expression in transfected MIC 216 cell lines. Total cellular proteins (25 μg or 50 μg per lane as indicated) were separated by reduced SDS-PAGE on 4–12% acrylamide gels, transferred to nitrocellulose, incubated with the polyclonal epimorphin/syntaxin 2 antibody, and then visualized with enhanced chemiluminescence reagent. (a) The 34-kDa band corresponds to the epimorphin/syntaxin 2 protein. MIC C: native MIC 216 cell line grown in media without G418. pCEN V: MIC 216 cells transfected with empty pCEN vector. (b) EpiAS1 and EpiAS2: Two independent clonal pCEN-antisense epimorphin/syntaxin 2 cell lines. (c) EpiS1, EpiS2, EpiS3, and EpiS5: Four independent clonal pCEN-epimorphin/syntaxin 2 (sense) transfected cell lines. (d) Quantitation of epimorphin/syntaxin 2 expression by NIH Image analysis of specific bands. C, control; V, vector; S, sense; AS, antisense.