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Citations to this article

Aortic endothelial cells regulate proliferation of human monocytes in vitro via a mechanism synergistic with macrophage colony-stimulating factor. Convergence at the cyclin E/p27(Kip1) regulatory checkpoint.
A S Antonov, … , R Virmani, R G Gerrity
A S Antonov, … , R Virmani, R G Gerrity
Published June 15, 1997
Citation Information: J Clin Invest. 1997;99(12):2867-2876. https://doi.org/10.1172/JCI119480.
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Research Article

Aortic endothelial cells regulate proliferation of human monocytes in vitro via a mechanism synergistic with macrophage colony-stimulating factor. Convergence at the cyclin E/p27(Kip1) regulatory checkpoint.

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Abstract

Monocyte-derived macrophages (Mphis) are pivotal participants in the pathogenesis of atherosclerosis. Evidence from both animal and human plaques indicates that local proliferation may contribute to accumulation of lesion Mphis, and the major Mphi growth factor, macrophage colony stimulating factor (MCSF), is present in atherosclerotic plaques. However, most in vitro studies have failed to demonstrate that human monocytes/Mphis possess significant proliferative capacity. We now report that, although human monocytes cultured in isolation showed only limited MCSF-induced proliferation, monocytes cocultured with aortic endothelial cells at identical MCSF concentrations underwent enhanced (up to 40-fold) and prolonged (21 d) proliferation. In contrast with monocytes in isolation, this was optimal at low seeding densities, required endothelial cell contact, and could not be reproduced by coculture with smooth muscle cells. Intimal Mphi isolated from human aortas likewise showed endothelial cell contact-dependent, MCSF-induced proliferation. Consistent with a two-signal mechanism governing Mphi proliferation, the cell cycle regulatory protein, cyclin E, was rapidly upregulated by endothelial cell contact in an MCSFindependent fashion, but MCSF was required for successful downregulation of the cell cycle inhibitory protein p27(Kip1) before cell cycling. Thus endothelial cells and MCSF differentially and synergistically regulate two Mphi genes critical for progression through the cell cycle.

Authors

A S Antonov, D H Munn, F D Kolodgie, R Virmani, R G Gerrity

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Citations to this article (32)

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I Coelho, N Duarte, MP Macedo, C Penha-Gonçalves
Journal of Clinical Medicine 2021
Proliferating SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis
C Morse, T Tabib, J Sembrat, KL Buschur, HT Bittar, E Valenzi, Y Jiang, DJ Kass, K Gibson, W Chen, A Mora, PV Benos, M Rojas, R Lafyatis
The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology 2019
LC3-Associated Phagocytosis and Inflammation
BL Heckmann, E Boada-Romero, LD Cunha, J Magne, DR Green
Journal of Molecular Biology 2017
An overview of AVF maturation and endothelial dysfunction in an advanced renal failure
MA Siddiqui, S Ashraff, D Santos, T Carline
Renal Replacement Therapy 2017
Monocyte Adhesion and Plaque Recruitment During Atherosclerosis Development Is Regulated by the Adapter Protein Chat-H/SHEP1Highlights
O Herbin, AG Regelmann, B Ramkhelawon, EG Weinstein, KJ Moore, K Alexandropoulos
Arteriosclerosis, thrombosis, and vascular biology 2016
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UM Becher, L Möller, V Tiyerili, MV Nicotera, F Hauptmann, K Zimmermann, A Pfeifer, G Nickenig, S Wassmann, N Werner
International Journal of Cardiology 2014
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TM Zotes, CF Arias, JJ Fuster, R Spada, S Pérez-Yagüe, E Hirsch, M Wymann, AC Carrera, V Andrés, DF Barber, E Lutgens
PloS one 2013
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FI Clanchy, JA Hamilton
Cytokine 2012
αVβ3 integrin regulates macrophage inflammatory responses via PI3 kinase/Akt-dependent NF-κB activation
AS Antonov, GN Antonova, DH Munn, N Mivechi, R Lucas, JD Catravas, AD Verin
Journal of Cellular Physiology 2010
Ischemia’s Proliferative and Morphological Effects: Analyzing the Roles of Hypoxia, Hypercapnia, and Glucose
AL Russ, DE Anderson, JJ McGill, KM Haberstroh, AE Rundell
Cellular and Molecular Bioengineering 2009
Heat shock protein 90 inhibitors protect and restore pulmonary endothelial barrier function
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American journal of respiratory cell and molecular biology 2008
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J Audoy-Remus, JF Richard, D Soulet, H Zhou, P Kubes, L Vallieres
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008
Lysosomal Cholesterol Accumulation Inhibits Subsequent Hydrolysis of Lipoprotein Cholesteryl Ester
WG Jerome, BE Cox, EE Griffin, JC Ullery
Microscopy and Microanalysis 2008
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Ping Zhou, Hideki Kitaura, Steven L Teitelbaum, Gerald Krystal, F Patrick Ross, Sunao Takeshita
Journal of immunology (Baltimore, Md. : 1950) 2006
Effect of serotonin on the differentiation of human monocytes into dendritic cells
N Katoh, F Soga, T Nara, R Tamagawa-Mineoka, M Nin, H Kotani, K Masuda, S Kishimoto
Clinical & Experimental Immunology 2006
Monocyte retention in the pathology of atherosclerosis
R Natarajan, Q Cai
Future Cardiology 2005
Regulation of macrophage foam cell formation by alphaVbeta3 integrin: potential role in human atherosclerosis
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The American Journal of Pathology 2004
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P González, A Díez-Juan, E Coto, V Alvarez, JR Reguero, A Batalla, V Andrés
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E Lukášová, Z Kořistek, M Falk, S Kozubek, S Grigoryev, M Kozubek, V Ondřej, I Kroupová
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A Díez-Juan, P Pérez, M Aracil, D Sancho, A Bernad, F Sánchez-Madrid, V Andrés
Blood 2004
Peripheral Blood “Endothelial Progenitor Cells” Are Derived From Monocyte/Macrophages and Secrete Angiogenic Growth Factors
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Circulation 2003
Lipid oxidation products and atherosclerosis
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Vascular Pharmacology 2002
GM-CSF: a strong arteriogenic factor acting by amplification of monocyte function
IR Buschmann, IE Hoefer, N Royen, E Katzer, R Braun-Dulleaus, M Heil, S Kostin, C Bode, W Schaper
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Journal of Hematotherapy & Stem Cell Research 2000
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Blood 1999
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I Deneux
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