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Citations to this article

The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39.
A J Marcus, … , R B Gayle, C R Maliszewski
A J Marcus, … , R B Gayle, C R Maliszewski
Published March 15, 1997
Citation Information: J Clin Invest. 1997;99(6):1351-1360. https://doi.org/10.1172/JCI119294.
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Research Article Article has an altmetric score of 9

The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39.

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Abstract

We previously demonstrated that when platelets are in motion and in proximity to endothelial cells, they become unresponsive to agonists (Marcus, A.J., L.B. Safier, K.A. Hajjar, H.L. Ullman, N. Islam, M.J. Broekman, and A.M. Eiroa. 1991. J. Clin. Invest. 88:1690-1696). This inhibition is due to an ecto-ADPase on the surface of endothelial cells which metabolizes ADP released from activated platelets, resulting in blockade of the aggregation response. Human umbilical vein endothelial cells (HUVEC) ADPase was biochemically classified as an E-type ATP-diphosphohydrolase. The endothelial ecto-ADPase is herein identified as CD39, a molecule originally characterized as a lymphoid surface antigen. All HUVEC ecto-ADPase activity was immunoprecipitated by monoclonal antibodies to CD39. Surface localization of HUVEC CD39 was established by confocal microscopy and flow cytometric analyses. Transfection of COS cells with human CD39 resulted in both ecto-ADPase activity as well as surface expression of CD39. PCR analyses of cDNA obtained from HUVEC mRNA and recombinant human CD39 revealed products of the same size, and of identical sequence. Northern blot analyses demonstrated that HUVEC express the same sized transcripts for CD39 as MP-1 cells (from which CD39 was originally cloned). We established the role of CD39 as a prime endothelial thromboregulator by demonstrating that CD39-transfected COS cells acquired the ability to inhibit ADP-induced aggregation in platelet-rich plasma. The identification of HUVEC ADPase/CD39 as a constitutively expressed potent inhibitor of platelet reactivity offers new prospects for antithrombotic therapeusis.

Authors

A J Marcus, M J Broekman, J H Drosopoulos, N Islam, T N Alyonycheva, L B Safier, K A Hajjar, D N Posnett, M A Schoenborn, K A Schooley, R B Gayle, C R Maliszewski

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Citations to this article in year 2012 (12)

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Nigel Mackman
Journal of Clinical Investigation 2012
Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
SH Visovatti, MC Hyman, D Bouis, R Neubig, VV McLaughlin, DJ Pinsky
PloS one 2012
New approaches for measurement of platelet reactivity
AJ Marcus
Blood 2012
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BA Thompson, MP Storm, J Hewinson, S Hogg, MJ Welham, AB MacKenzie
Cellular Signalling 2012
Adenosine 5′-triphosphate (ATP) supplements are not orally bioavailable: a randomized, placebo-controlled cross-over trial in healthy humans
IC Arts, EJ Coolen, MJ Bours, N Huyghebaert, MA Stuart, A Bast, PC Dagnelie
Journal of the International Society of Sports Nutrition 2012
The effect of reactive oxygen species on whole blood aggregation and the endothelial cell-platelet interaction in patients with coronary heart disease
J Watt, MA Ewart, FH Greig, KG Oldroyd, RM Wadsworth, S Kennedy
Thrombosis Research 2012
The role of the NTPDase enzyme family in parasites: what do we know, and where to from here?
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Platelet-mediated vascular dysfunction during acute lung injury
JT Dixon, E Gozal, AM Roberts
Archives Of Physiology And Biochemistry 2012
NPP4 is a procoagulant enzyme on the surface of vascular endothelium
RA Albright, WC Chang, D Robert, DL Ornstein, W Cao, L Liu, ME Redick, JI Young, EM de la Cruz, DT Braddock
Blood 2012
Spatio-temporal expression of PsAPY1 during germination, differentiation, and organogenesis in pea (Pisum sativum L.Var. Alaska)
T Sharma, VP Sahi, EH Morita, S Abe
Acta Physiologiae Plantarum 2012
Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
SH Visovatti, MC Hyman, D Bouis, R Neubig, VV McLaughlin, DJ Pinsky, HK Eltzschig
PloS one 2012
Antiplatelet and Anticoagulation Therapy
A Ferro, DA Garcia
2012

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