Since transglutaminases create covalent gamma-glutamyl-epsilon-lysine cross-links between extracellular matrix proteins they are prime candidates for stabilizing tissue during wound healing. Therefore, we studied the temporo-spatial expression of transglutaminase activity in skin regenerating from cultured epithelial autografts in severely burned children by the specific incorporation of monodansylcadaverine into cryostat sections from skin biopsies obtained between 5 d to 17 mo after grafting. The dansyl label was subsequently immunolocalized in the epidermis, dermal connective tissue, and along the basement membrane. Incubation of cryosections of normal and regenerating skin with purified tissue transglutaminase confirmed the dermo-epidermal junction and the papillary dermis as targets for this enzyme and revealed that in regenerating skin transamidation of the basement membrane zone was completed only 4-5 mo after grafting. Immunoelectron microscopy revealed that three distinct regions on the central portion of anchoring fibrils were positive for monodansylcadaverine in normal skin which were negative during the initial phase of de novo formation of anchoring fibrils in regenerating skin. Biochemically, we identified collagen VII as potential substrate for tissue transglutaminase. Thus, tissue transglutaminase appears to play an important role not only in cross-linking of the papillary dermis but also of the dermo-epidermal junction in particular.
M Raghunath, B Höpfner, D Aeschlimann, U Lüthi, M Meuli, S Altermatt, R Gobet, L Bruckner-Tuderman, B Steinmann
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Mesenchymal Transglutaminase 2 Activates Epithelial ADAM17: Link to G-Protein-Coupled Receptor 56 (ADGRG1) Signalling.
Bauer L, Edwards J, Heil A, Dewitt S, Biebermann H, Aeschlimann D, Knäuper V |
International journal of molecular sciences | 2024 |
Long-Term Outcomes of a Cultured Autologous Dermo-Epidermal Skin Substitute in Children: 5-Year Results of a Phase I Clinical Trial
Schiestl C, Neuhaus K, Meuli M, Farkas M, Hartmann-Fritsch F, Elrod J, Bressan J, Reichmann E, Böttcher-Haberzeth S |
Journal of Burn Care & Research: Official Publication of the American Burn Association | 2024 |
Dystrophic Epidermolysis Bullosa: Secondary Disease Mechanisms and Disease Modifiers
A Nyström, L Bruckner-Tuderman, D Kiritsi |
Frontiers in Genetics | 2021 |
The Collagen Superfamily and Collagenopathies
F Ruggiero |
2021 | |
Gene expression advances skin reconstruction and wound repair better on silk fibroin-based materials than on collagen-based materials
T Hashimoto, K Kojima, Y Tamada |
2020 | |
Sensitivity of Transglutaminase 3 in the IgA Aggregates in Dermatitis Herpetiformis Skin to Potassium Iodide
TB Taylor, JJ Zone |
Journal of Investigative Dermatology | 2018 |
The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development
A Sorushanova, LM Delgado, Z Wu, N Shologu, A Kshirsagar, R Raghunath, AM Mullen, Y Bayon, A Pandit, M Raghunath, DI Zeugolis |
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Cell Engineering and Regeneration
JM Gimble, D Marolt, R Oreffo, H Redl, S Wolbank |
Cell Engineering and Regeneration | 2017 |
Collagen VII Half-Life at the Dermal-Epidermal Junction Zone: Implications for Mechanisms and Therapy of Genodermatoses
T Kühl, M Mezger, I Hausser, LT Guey, R Handgretinger, L Bruckner-Tuderman, A Nyström |
Journal of Investigative Dermatology | 2016 |
Wound Healing Biomaterials
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Characterization of TG2 and TG1–TG2 double knock-out mouse epidermis
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Amino Acids | 2016 |
Loss of Collagen VII Is Associated with Reduced Transglutaminase 2 Abundance and Activity
V Küttner, C Mack, C Gretzmeier, L Bruckner-Tuderman, J Dengjel |
Journal of Investigative Dermatology | 2014 |
Making More Matrix: Enhancing the deposition of dermal-epidermal junction components in vitro and accelerating organotypic skin culture development, using macromolecular crowding
P Benny, C Badowski, B Lane, M Raghunath |
Tissue Engineering Part A | 2014 |
Enhanced transglutaminase 2 expression in response to stress-related catecholamines in macrophages
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Immunobiology | 2014 |
The vWFA2 domain of type VII collagen is responsible for collagen binding
H Wegener, S Leineweber, K Seeger |
Biochemical and Biophysical Research Communications | 2013 |
Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier
S John, L Thiebach, C Frie, S Mokkapati, M Bechtel, R Nischt, S Rosser-Davies, M Paulsson, N Smyth |
PloS one | 2012 |
Transglutaminase 2 and NF-κB: an odd couple that shapes breast cancer phenotype
KD Brown |
Breast Cancer Research and Treatment | 2012 |
Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier
S John, L Thiebach, C Frie, S Mokkapati, M Bechtel, R Nischt, S Rosser-Davies, M Paulsson, N Smyth, JM Brandner |
PloS one | 2012 |
Transglutaminases (TGs) in Ocular and Periocular Tissues: Effect of Muscarinic Agents on TGs in Scleral Fibroblasts
VA Barathi, SR Weon, QS Tan, KJ Lin, L Tong, RW Beuerman, N Sharif |
PloS one | 2011 |
Tissue transglutaminase inhibits the TRPV5-dependent calcium transport in an N-glycosylation-dependent manner
S Boros, , H Dimke, AW van der Kemp, K Tudpor, S Verkaart, KP Lee, RJ Bindels, JG Hoenderop |
Cellular and Molecular Life Sciences | 2011 |
Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies
C Chen, F Loe, A Blocki, Y Peng, M Raghunath |
Advanced Drug Delivery Reviews | 2011 |
Tissue transglutaminase inhibits the TRPV5-dependent calcium transport in an N-glycosylation-dependent manner
S Boros, Q Xi, H Dimke, AW Kemp, K Tudpor, S Verkaart, KP Lee, RJ Bindels, JG Hoenderop |
Cellular and Molecular Life Sciences | 2011 |
Tissue adhesives in ocular surgery
HC Park, R Champakalakshmi, PP Panengad, M Raghunath, JS Mehta |
Expert Review of Ophthalmology | 2011 |
Skingineering I: engineering porcine dermo-epidermal skin analogues for autologous transplantation in a large animal model
E Braziulis, T Biedermann, F Hartmann-Fritsch, C Schiestl, L Pontiggia, S Böttcher-Haberzeth, E Reichmann, M Meuli |
Pediatric Surgery International | 2010 |
Dystrophic Epidermolysis Bullosa: Pathogenesis and Clinical Features
L Bruckner-Tuderman |
Dermatologic Clinics | 2010 |
Modular enzymatically crosslinked protein polymer hydrogels for in situ gelation
NE Davis, S Ding, RE Forster, DM Pinkas, AE Barron |
Biomaterials | 2010 |
Current Topics in Membranes
TC Wagenknecht, Z Liu |
Structure and Function of Calcium Release Channels | 2010 |
Increased TG2 expression can result in induction of transforming growth factor beta1, causing increased synthesis and deposition of matrix proteins, which can be regulated by nitric oxide
D Telci, RJ Collighan, H Basaga, M Griffin |
The Journal of biological chemistry | 2009 |
Transglutaminases, involucrin, and loricrin as markers of epidermal differentiation in skin substitutes derived from human sweat gland cells
S Tharakan, L Pontiggia, T Biedermann, S Böttcher-Haberzeth, C Schiestl, E Reichmann, M Meuli |
Pediatric Surgery International | 2009 |
Increased TG2 Expression Can Result in Induction of Transforming Growth Factor β1, Causing Increased Synthesis and Deposition of Matrix Proteins, Which Can Be Regulated by Nitric Oxide
D Telci, RJ Collighan, H Basaga, M Griffin |
The Journal of biological chemistry | 2009 |
13th International Conference on Biomedical Engineering
CT Lim, JC Goh |
2009 | |
Transglutaminase 2 cross-linking of matrix proteins: biological significance and medical applications
RJ Collighan, M Griffin |
Amino Acids | 2008 |
Supramolecular Interactions in the Dermo-epidermal Junction Zone: ANCHORING FIBRIL-COLLAGEN VII TIGHTLY BINDS TO BANDED COLLAGEN FIBRILS
D Villone, A Fritsch, M Koch, L Bruckner-Tuderman, U Hansen, P Bruckner |
The Journal of biological chemistry | 2008 |
Biomedical and Biotechnological Applications of Elastin-Like Polypeptides
AJ Simnick, DW Lim, D Chow, A Chilkoti |
Polymer Reviews | 2007 |
Transglutaminase inhibitors induce hyperproliferation and parakeratosis in tissue-engineered skin
CA Harrison, CM Layton, Z Hau, AJ Bullock, TS Johnson, S MacNeil |
British Journal of Dermatology | 2007 |
Foodborne Diseases
S Simjee |
2007 | |
Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute
K YAMAZOE, S MIYAMOTO, Y HIKOSAKA, K KITAGAWA, K WATANABE, H SAKAI, T KUDO |
Journal of Veterinary Medical Science | 2007 |
Advances in Enzymology - and Related Areas of Molecular Biology
H Waelsch |
Advances in Enzymology and Related Areas of Molecular Biology | 2006 |
Synthesis andin VitroEvaluation of Enzymatically Cross-Linked Elastin-Like Polypeptide Gels for Cartilaginous Tissue Repair
MK McHale, LA Setton, A Chilkoti |
Tissue Engineering | 2005 |
Immunoglobulin, complement and epidermal transglutaminase deposition in the cutaneous vessels in dermatitis herpetiformis
K Preisz, M Sardy, A Horvath, S Karpati |
Journal of the European Academy of Dermatology and Venereology | 2005 |
Autoimmune Diseases of the Skin
M Hertl |
2005 | |
Crosslinking and G-protein functions of transglutaminase 2 contribute differentially to fibroblast wound healing responses
P Stephens, P Grenard, P Aeschlimann, M Langley, E Blain, R Errington, D Kipling, D Thomas, D Aeschlimann |
Journal of cell science | 2004 |
Molecular Basis of Elastic Fiber Formation: CRITICAL INTERACTIONS AND A TROPOELASTIN-FIBRILLIN-1 CROSS-LINK
MJ Rock, SA Cain, LJ Freeman, A Morgan, K Mellody, A Marson, CA Shuttleworth, AS Weiss, CM Kielty |
The Journal of biological chemistry | 2004 |
Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet A Source
SR Gross, Z Balklava, M Griffin |
Journal of Investigative Dermatology | 2003 |
In patients with dermatitis herpetiformis distribution of transglutaminase in cutaneous tissue does not differ from controls
F Biagi, E Bassi, M Ardigó, MA Vignini, M Caravaggi, G Borroni, GR Corazza |
Digestive and Liver Disease | 2003 |
Transglutaminases: crosslinking enzymes with pleiotropic functions
L Lorand, RM Graham |
Nature Reviews Molecular Cell Biology | 2003 |
Recognition and Management of the Cutaneous Manifestations of Celiac Disease: A Guide for Dermatologists
P Collin, T Reunala |
American Journal of Clinical Dermatology | 2003 |
Tissue transglutaminase localization and activity regulation in the extracellular matrix of articular cartilage
BT Summey, RD Graff, TS Lai, CS Greenberg, GM Lee |
Journal of Orthopaedic Research | 2002 |
Enhanced expression of transglutaminase 2 in anterior polar cataracts and its induction by TGF-beta in vitro
XH Wan, EH Lee, HJ Koh, J Song, EK Kim, CY Kim, JB Lee, SY Kim, K Yao, JH Lee |
The British journal of ophthalmology | 2002 |
Transglutaminases in disease
SY Kim, TM Jeitner, PM Steinert |
Neurochemistry International | 2002 |
Injury-induced ‘switch’ from GTP-regulated to novel GTP-independent isoform of tissue transglutaminase in the rat spinal cord
BW Festoff, K SantaCruz, PM Arnold, CT Sebastian, PJ Davies, BA Citron |
Journal of Neurochemistry | 2002 |
Increases in Renal ε-(γ-Glutamyl)-Lysine Crosslinks Result from Compartment-Specific Changes in Tissue Transglutaminase in Early Experimental Diabetic Nephropathy: Pathologic Implications
NJ Skill, M Griffin, AM el Nahas, T Sanai, JL Haylor, M Fisher, MF Jamie, NN Mould, TS Johnson |
Laboratory Investigation | 2001 |
Dermatitis herpetiformis
TL Reunala |
Clinics in Dermatology | 2001 |
Transglutaminase-mediated cross-linking is involved in the stabilization of extracellular matrix in human liver fibrosis
P Grenard, S Bresson-Hadni, SE Alaoui, M Chevallier, DA Vuitton, S Ricard-Blum |
Journal of Hepatology | 2001 |
Facilitated Wound Healing by Activation of the Transglutaminase 1 Gene
R Inada, M Matsuki, K Yamada, Y Morishima, SC Shen, N Kuramoto, H Yasuno, K Takahashi, Y Miyachi, K Yamanishi |
The American Journal of Pathology | 2000 |
Protein Crosslinking in Assembly and Remodelling of Extracellular Matrices: The Role of Transglutaminases
D Aeschlimann, V Thomazy |
Connective Tissue Research | 2000 |
Quantitative determination of ceramide using diglyceride kinase
DK Perry, A Bielawska, YA Hannun |
Methods in enzymology | 2000 |
Chicken Coagulation Factor XIIIA Is Produced by the Theca Externa and Stabilizes the Ovarian Follicular Wall
B Recheis, A Osanger, S Haubenwallner, WJ Schneider, J Nimpf |
The Journal of biological chemistry | 2000 |
Tissue-specific expression and long-term deposition of human collagen VII in the skin of transgenic mice: implications for gene therapy
E Sat, KH Leung, L Bruckner-Tuderman, KS Cheah |
Gene Therapy | 2000 |
Transient Expression of Transglutaminase C During Prenatal Development of Human Muscles
SK Lee, JG Chi, SC Park, SI Chung |
Journal of Histochemistry & Cytochemistry | 2000 |
Sporadic Inclusion Body Myositis Correlates with Increased Expression and Cross-linking by Transglutaminases 1 and 2
YC Choi, GT Park, TS Kim, IN Sunwoo, PM Steinert, SY Kim |
The Journal of biological chemistry | 2000 |
Implication of tissue transglutaminase and desmoplakin in cell adhesion mechanism in human epidermis
C Esposito, M L Lombardi, V Ruocco, A Cozzolino, L Mariniello, R Porta |
Molecular and Cellular Biochemistry | 2000 |
Biology of anchoring fibrils: lessons from dystrophic epidermolysis bullosa
L Bruckner-Tuderman, B Höpfner, N Hammami-Hauasli |
Matrix Biology | 1999 |
Antibodies to Tissue Transglutaminase as Serologic Markers in Patients with Dermatitis Herpetiformis
W Dieterich, E Laag, L Bruckner-Tuderman, T Reunala, S Karpati, T Zagoni, EO Riecken, D Schuppan |
Journal of Investigative Dermatology | 1999 |
Distribution and activity of transglutaminase in rat brain carcinogenesis and in gliomas
P Tunici, A Sessa, E Rabellotti, A Calloni, A Perin |
Cancer Letters | 1999 |
Hereditary skin diseases of anchoring fibrils
L Bruckner-Tuderman |
Journal of Dermatological Science | 1999 |
Tissue transglutaminase is expressed, active, and directly involved in rat dermal wound healing and angiogenesis
ZA Haroon, JM Hettasch, TS Lai, MW Dewhirst, CS Greenberg |
The FASEB Journal | 1999 |
Tissue Transglutaminase Is an Important Player at the Surface of Human Endothelial Cells: Evidence for Its Externalization and Its Colocalization with the β1 Integrin
CA Gaudry, E Verderio, RA Jones, C Smith, M Griffin |
Experimental Cell Research | 1999 |
Regulation of Cell Surface Tissue Transglutaminase: Effects on Matrix Storage of Latent Transforming Growth Factor-β Binding Protein-1
E Verderio, C Gaudry, S Gross, C Smith, S Downes, M Griffin |
Journal of Histochemistry & Cytochemistry | 1999 |
Cell Surface Localization of Tissue Transglutaminase Is Dependent on a Fibronectin-binding Site in Its N-terminal β-Sandwich Domain
CA Gaudry, E Verderio, D Aeschlimann, A Cox, C Smith, M Griffin |
The Journal of biological chemistry | 1999 |
Transient Bullous Dermolysis of the Newborn Associated with Compound Heterozygosity for Recessive and Dominant COL7A1 Mutations
N Hammami-Hauasli, M Raghunath, W Küster, L Bruckner-Tuderman |
Journal of Investigative Dermatology | 1998 |
Immunohistochemical Study of Type I Collagen Turn-over and of Matrix Metalloproteinases in Chromoblastomycosis Before and After Treatment by Terbinafine
P Esterre, L Risteli, S Ricard-Blum |
Pathology - Research and Practice | 1998 |
Regulated Expression of Tissue Transglutaminase in Swiss 3T3 Fibroblasts: Effects on the Processing of Fibronectin, Cell Attachment, and Cell Death
E Verderio, B Nicholas, S Gross, M Griffin |
Experimental Cell Research | 1998 |
Isolation of a cDNA Encoding a Novel Member of the Transglutaminase Gene Family from Human Keratinocytes: DETECTION AND IDENTIFICATION OF TRANSGLUTAMINASE GENE PRODUCTS BASED ON REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION WITH DEGENERATE PRIMERS
D Aeschlimann, MK Koeller, BL Allen-Hoffmann, DF Mosher |
The Journal of biological chemistry | 1998 |
Modulation of the in Situ Activity of Tissue Transglutaminase by Calcium and GTP
J Zhang, M Lesort, RP Guttmann, GV Johnson |
The Journal of biological chemistry | 1998 |
Some, but Not All, Glycine Substitution Mutations in COL7A1 Result in Intracellular Accumulation of Collagen VII, Loss of Anchoring Fibrils, and Skin Blistering
N Hammami-Hauasli, H Schumann, M Raghunath, O Kilgus, U Lüthi, T Luger, L Bruckner-Tuderman |
The Journal of biological chemistry | 1998 |
Three Novel Homozygous Point Mutations and a New Polymorphism in the COL17A1 Gene: Relation to Biological and Clinical Phenotypes of Junctional Epidermolysis Bullosa
H Schumann, N Hammami-Hauasli, L Pulkkinen, A Mauviel, W Küster, U Lüthi, K Owaribe, J Uitto, L Bruckner-Tuderman |
The American Journal of Human Genetics | 1997 |
Efficacy of cultured epithelial autografts in pediatric burns and reconstructive surgery
R Gobet, M Raghunath, S Altermatt, C Meuli-Simmen, M Benathan, A Dietl, M Meuli |
Surgery | 1997 |
Expression of tissue transglutaminase in human bladder carcinoma
H Hager, PH Jensen, S Hamilton-Dutoit, MS Nielsen, P Birckbichler, J Gliemann |
The Journal of Pathology | 1997 |
Protein Cross-linking Mediated by Tissue Transglutaminase Correlates with the Maturation of Extracellular Matrices During Lung Development
JC Schittny, M Paulsson, C Vallan, PH Burri, N Kedei, D Aeschlimann |
American journal of respiratory cell and molecular biology | 1997 |
Cultured epithelial autografts: diving from surgery into matrix biology
M Raghunath, M Meuli |
Pediatric Surgery International | 1997 |
International Review of Cytology
RA Miller |
International Review of Cytology | 1991 |