The effect of proadrenomedullin NH2-terminal 20 peptide (PAMP) on the voltage-gated Ca2+ channel current was investigated using the perforated whole-cell clamp technique on NGF-treated PC12 cells. PAMP inhibited the Ba2+ current through N-type Ca2+ channels in a concentration dependent manner. Injection of GDPbetaS into the cell abolished the inhibition while injection of GTPgammaS into the cell made the inhibition irreversible, indicating that the PAMP-induced inhibition of the voltage-gated Ca2+ channel was mediated by a G protein. The inhibition was abolished by pretreating the cells with pertussis toxin, indicating that a pertussis toxin-sensitive G protein was involved in the signal transduction mechanism of PAMP. The present study revealed that the inhibition of catecholamine secretion from sympathetic nerve endings by PAMP could be explained by the inhibition of N-type Ca2+ channels, which was mediated by pertussis toxin-sensitive G protein.
K Takano, N Yamashita, T Fujita
Title and authors | Publication | Year |
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ZC Chroneos, Z Sever-Chroneos, VL Shepherd |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 2009 |
Molecular Mechanisms in Hypertension
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Molecular Mechanisms in Hypertension: paracrine regulator of salt sensitivity and blood pressure | 2006 |
Adrenomedullin and its Related Peptide
T SHIMOSAWA, T FUJITA |
Endocrine Journal | 2005 |
Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides
M Kamohara, A Matsuo, J Takasaki, M Kohda, M Matsumoto, S Matsumoto, T Soga, H Hiyama, M Kobori, M Katou |
Biochemical and Biophysical Research Communications | 2005 |
Interleukin 1β Modulates Rat Subfornical Organ Neurons as a Result of Activation of a Non-Selective Cationic Conductance
SE Desson, AV Ferguson |
The Journal of Physiology | 2003 |
A review of the biological properties and clinical implications of adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP), hypotensive and vasodilating peptides
T Eto |
Peptides | 2001 |
Adrenomedullin and central cardiovascular regulation
MM Taylor, WK Samson |
Peptides | 2001 |
Novel actions of proadrenomedullin N-terminal 20 peptide (PAMP)
K Ohinata, A Inui, A Asakawa, M Yoshikawa |
Peptides | 2001 |
Adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP) in adrenal chromaffin cells
H Kobayashi, T Yanagita, H Yokoo, A Wada |
Peptides | 2001 |
Selective inhibition of nicotinic cholinergic receptors by proadrenomedullin N-terminal 12 peptide in bovine adrenal chromaffin cells
H Kobayashi, R Yamamoto, K Kitamura, K Kuwasako, S Minami, T Yanagita, S Shiraishi, H Yokoo, T Eto, A Wada |
Molecular Brain Research | 2001 |
Current topics in pheochromocytoma
K Nakao, H Itoh, K Takaya |
Biomedecine & Pharmacotherapy | 2000 |
Effects of adrenomedullin and PAMP on membrane potential and neurotransmission☆
K Goto, K Fujii, U Onaka, I Abe, M Fujishima |
Peptides | 2000 |
Proadrenomedullin NH2-terminal 20 peptide (PAMP) and adrenomedullin bind to teratocarcinoma cells☆
TW Moody, D Coy, F Cuttitta, LM Montuenga |
Peptides | 2000 |
ADRENOMEDULLIN AND THE CONTROL OF FLUID AND ELECTROLYTE HOMEOSTASIS
WK Samson |
Annual Review of Physiology | 1999 |
Effects of PAMP on mRNAs coding for catecholamine-synthesizing enzymes in PC12 cells
K Takekoshi, K Isobe, F Nomura, M Motooka, T Nanmoku, T Nakai |
Life Sciences | 1999 |
Effects of adrenomedullin and PAMP on adrenal catecholamine release in dogs
K Masada, T Nagayama, A Hosokawa, M Yoshida, M Suzuki-Kusaba, H Hisa, T Kimura, S Satoh |
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology | 1999 |
Proadrenomedullin N-Terminal 20 Peptide Hyperpolarizes the Membrane by Activating an Inwardly Rectifying K + Current in Differentiated PC12 Cells
K Takano, T Fujita |
Circulation research | 1999 |
Hormones and the Heart in Health and Disease
L Share |
1999 | |
Proadrenomedullin-Derived Peptides
WK Samson |
Frontiers in Neuroendocrinology | 1998 |
Proadrenomedullin N-Terminal 20 Peptide: Minimal Active Region to Regulate Nicotinic Receptors
M Mahata, SK Mahata, RJ Parmer, DT OConnor |
Hypertension | 1998 |
Effects of a Novel Antihypertensive Drug, Cilnidipine, on Catecholamine Secretion From Differentiated PC12 Cells
H Uneyama, H Uchida, R Yoshimoto, S Ueno, K Inoue, N Akaike |
Hypertension | 1998 |
Endocrinology of Cardiovascular Function
ER Levin, JL Nadler |
1998 | |
Proadrenomedullin N-Terminal 20 Peptide Inhibits Adrenocorticotropin Secretion from Cultured Pituitary Cells, Possibly via Activation of a Potassium Channel
WK Samson, TC Murphy, ZT Resch |
Endocrine | 1998 |
Proadrenomedullin N-terminal 20 peptide (PAMP) inhibits proliferation of human neuroblastoma TGW cells
K Ando, N Omi, T Shimosawa, T Fujita |
FEBS Letters | 1997 |
Role of Adrenomedullin and Related Peptides in the Regulation of the Hypothalamo–Pituitary–Adrenal Axis
GG Nussdorfer, GP Rossi, G Mazzocchi |
Peptides | 1997 |
International Review of Cytology
RA Miller |
International Review of Cytology | 1991 |
The effect of adrenomedullin and proadrenomedullin N-terminal 20 peptide on angiotensin II induced vascular smooth muscle cell proliferation
Jian Ma, Yinglu Feng, Zaiquan Li, Chaoshu Tang |
Iranian Journal of Basic Medical Sciences | |
A Newly Identified Peptide, Proadrenomedullin N-Terminal 20 Peptide, Induces Hypotensive Action via Pertussis Toxin–Sensitive Mechanisms
T Shimosawa, K Ando, T Fujita |
Hypertension | 1997 |