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Citations to this article

Proadrenomedullin NH2-terminal 20 peptide inhibits the voltage-gated Ca2+ channel current through a pertussis toxin-sensitive G protein in rat pheochromocytoma-derived PC 12 cells.
K Takano, … , N Yamashita, T Fujita
K Takano, … , N Yamashita, T Fujita
Published July 1, 1996
Citation Information: J Clin Invest. 1996;98(1):14-17. https://doi.org/10.1172/JCI118758.
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Research Article

Proadrenomedullin NH2-terminal 20 peptide inhibits the voltage-gated Ca2+ channel current through a pertussis toxin-sensitive G protein in rat pheochromocytoma-derived PC 12 cells.

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Abstract

The effect of proadrenomedullin NH2-terminal 20 peptide (PAMP) on the voltage-gated Ca2+ channel current was investigated using the perforated whole-cell clamp technique on NGF-treated PC12 cells. PAMP inhibited the Ba2+ current through N-type Ca2+ channels in a concentration dependent manner. Injection of GDPbetaS into the cell abolished the inhibition while injection of GTPgammaS into the cell made the inhibition irreversible, indicating that the PAMP-induced inhibition of the voltage-gated Ca2+ channel was mediated by a G protein. The inhibition was abolished by pretreating the cells with pertussis toxin, indicating that a pertussis toxin-sensitive G protein was involved in the signal transduction mechanism of PAMP. The present study revealed that the inhibition of catecholamine secretion from sympathetic nerve endings by PAMP could be explained by the inhibition of N-type Ca2+ channels, which was mediated by pertussis toxin-sensitive G protein.

Authors

K Takano, N Yamashita, T Fujita

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Total citations by year

Year: 2015 2009 2006 2005 2003 2001 2000 1999 1998 1997 1991 Total
Citations: 2 1 1 2 1 5 3 5 5 3 1 29
Citation information
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Citations to this article (30)

Title and authors Publication Year
Muscarinic inhibition of nicotinic transmission in rat sympathetic neurons and adrenal chromaffin cells
LL He, QF Zhang, LC Wang, JX Dai, CH Wang, LH Zheng, Z Zhou
Philosophical Transactions of the Royal Society B: Biological Sciences 2015
Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)
L Kőhidai, K Tóth, P Samotik, K Ranganathan, O Láng, M Tóth, H Ruskoaho
Molecular and Cellular Biochemistry 2015
Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2009
Molecular Mechanisms in Hypertension
T Shimosawa, T Fujita, R Watson, S Supowit, D DiPette
Molecular Mechanisms in Hypertension: paracrine regulator of salt sensitivity and blood pressure 2006
Adrenomedullin and its Related Peptide
T SHIMOSAWA, T FUJITA
Endocrine Journal 2005
Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides
M Kamohara, A Matsuo, J Takasaki, M Kohda, M Matsumoto, S Matsumoto, T Soga, H Hiyama, M Kobori, M Katou
Biochemical and Biophysical Research Communications 2005
Interleukin 1β Modulates Rat Subfornical Organ Neurons as a Result of Activation of a Non-Selective Cationic Conductance
SE Desson, AV Ferguson
The Journal of Physiology 2003
A review of the biological properties and clinical implications of adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP), hypotensive and vasodilating peptides
T Eto
Peptides 2001
Adrenomedullin and central cardiovascular regulation
MM Taylor, WK Samson
Peptides 2001
Novel actions of proadrenomedullin N-terminal 20 peptide (PAMP)
K Ohinata, A Inui, A Asakawa, M Yoshikawa
Peptides 2001
Adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP) in adrenal chromaffin cells
H Kobayashi, T Yanagita, H Yokoo, A Wada
Peptides 2001
Selective inhibition of nicotinic cholinergic receptors by proadrenomedullin N-terminal 12 peptide in bovine adrenal chromaffin cells
H Kobayashi, R Yamamoto, K Kitamura, K Kuwasako, S Minami, T Yanagita, S Shiraishi, H Yokoo, T Eto, A Wada
Molecular Brain Research 2001
Current topics in pheochromocytoma
K Nakao, H Itoh, K Takaya
Biomedecine & Pharmacotherapy 2000
Effects of adrenomedullin and PAMP on membrane potential and neurotransmission☆
K Goto, K Fujii, U Onaka, I Abe, M Fujishima
Peptides 2000
Proadrenomedullin NH2-terminal 20 peptide (PAMP) and adrenomedullin bind to teratocarcinoma cells☆
TW Moody, D Coy, F Cuttitta, LM Montuenga
Peptides 2000
ADRENOMEDULLIN AND THE CONTROL OF FLUID AND ELECTROLYTE HOMEOSTASIS
WK Samson
Annual Review of Physiology 1999
Effects of PAMP on mRNAs coding for catecholamine-synthesizing enzymes in PC12 cells
K Takekoshi, K Isobe, F Nomura, M Motooka, T Nanmoku, T Nakai
Life Sciences 1999
Effects of adrenomedullin and PAMP on adrenal catecholamine release in dogs
K Masada, T Nagayama, A Hosokawa, M Yoshida, M Suzuki-Kusaba, H Hisa, T Kimura, S Satoh
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 1999
Proadrenomedullin N-Terminal 20 Peptide Hyperpolarizes the Membrane by Activating an Inwardly Rectifying K + Current in Differentiated PC12 Cells
K Takano, T Fujita
Circulation research 1999
Hormones and the Heart in Health and Disease
L Share
1999
Proadrenomedullin-Derived Peptides
WK Samson
Frontiers in Neuroendocrinology 1998
Proadrenomedullin N-Terminal 20 Peptide: Minimal Active Region to Regulate Nicotinic Receptors
M Mahata, SK Mahata, RJ Parmer, DT OConnor
Hypertension 1998
Effects of a Novel Antihypertensive Drug, Cilnidipine, on Catecholamine Secretion From Differentiated PC12 Cells
H Uneyama, H Uchida, R Yoshimoto, S Ueno, K Inoue, N Akaike
Hypertension 1998
Endocrinology of Cardiovascular Function
ER Levin, JL Nadler
1998
Proadrenomedullin N-Terminal 20 Peptide Inhibits Adrenocorticotropin Secretion from Cultured Pituitary Cells, Possibly via Activation of a Potassium Channel
WK Samson, TC Murphy, ZT Resch
Endocrine 1998
Proadrenomedullin N-terminal 20 peptide (PAMP) inhibits proliferation of human neuroblastoma TGW cells
K Ando, N Omi, T Shimosawa, T Fujita
FEBS Letters 1997
Role of Adrenomedullin and Related Peptides in the Regulation of the Hypothalamo–Pituitary–Adrenal Axis
GG Nussdorfer, GP Rossi, G Mazzocchi
Peptides 1997
International Review of Cytology
RA Miller
International Review of Cytology 1991
The effect of adrenomedullin and proadrenomedullin N-terminal 20 peptide on angiotensin II induced vascular smooth muscle cell proliferation
Jian Ma, Yinglu Feng, Zaiquan Li, Chaoshu Tang
Iranian Journal of Basic Medical Sciences
A Newly Identified Peptide, Proadrenomedullin N-Terminal 20 Peptide, Induces Hypotensive Action via Pertussis Toxin–Sensitive Mechanisms
T Shimosawa, K Ando, T Fujita
Hypertension 1997

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