Expression of the dihydropyridine (DHP) receptor (alpha 1 subunit of L-type calcium channel) in heart is regulated by differentiation and innervation and is altered in congestive heart failure. We examined the transmembrane signaling pathways by which norepinephrine regulates DHP receptor expression in cultured neonatal rat ventricular myocytes. Using a 1.3-kb rat cardiac DHP receptor probe, and Northern analysis quantified by laser densitometry, we found that norepinephrine exposure produced a 2.2-fold increase in DHP receptor mRNA levels at 2 h followed by a decline to 50% of control at 4-48 h (P < 0.02). The alpha-adrenergic agonist phenylephrine and a phorbol ester produced a decline in mRNA levels (8-48 h). The beta-adrenergic agonist isoproterenol and 8-bromo-cAMP produced a transient increase in mRNA levels. After 24 h of exposure to isoproterenol, 3H-(+)PN200-110 binding sites increased from 410 +/- 8 to 539 +/- 39 fmol/mg (P < 0.05). The number of functional calcium channels, estimated by whole-cell voltage clamp experiments, was also increased after 24 h of exposure to isoproterenol. Peak current density (recordings performed in absence of isoproterenol) increased from -10.8 +/- 0.8 (n = 23) to -13.9 +/- 1.0 pA/pF (n = 27) (P < 0.01). Other characteristics of the calcium current (voltage for peak current, activation, and inactivation) were unchanged. Exposure for 48 h to phenylephrine produced a significant decline in peak current density (P < 0.01). We conclude that beta -adrenergic transmembrane signaling increases DHP receptor mRNA and number of functional calcium channels and that alpha - adrenergic transmembrane signaling produces a reciprocal effect. Regulation of cardiac calcium channel expression by adrenergic pathways may have physiological and pathophysiological importance.
T Maki, E J Gruver, A J Davidoff, N Izzo, D Toupin, W Colucci, A R Marks, J D Marsh
Title and authors | Publication | Year |
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The Dysfunctional Scenario of the Major Components Responsible for Myocardial Calcium Balance in Heart Failure Induced by Aortic Stenosis.
Silva VLD, Souza SLB, Mota GAF, Campos DHS, Melo AB, Vileigas DF, Sant'Ana PG, Coelho PM, Bazan SGZ, Leopoldo AS, Cicogna AC |
Arquivos brasileiros de cardiologia | 2022 |
Role of defective calcium regulation in cardiorespiratory dysfunction in Huntington’s disease
Haikel Dridi, Xiaoping Liu, Qi Yuan, Steve Reiken, Yehya Mohamad, Leah R. Sittenfeld, Panagiota Apostolou, Julie Buron, Pierre Sicard, Stefan Matecki, Jérôme Thireau, Clement Menuet, Alain Lacampagne, Andrew R. Marks |
JCI Insight | 2020 |
The calcium channel subunit gamma-4 is regulated by MafA and necessary for pancreatic beta-cell specification
C Luan, Y Ye, T Singh, M Barghouth, L Eliasson, I Artner, E Zhang, E Renström |
2019 | |
Linagliptin Suppresses Electrical and Structural Remodeling in the Isoproterenol Induced Myocardial Injury Model
N Ishizue, S Niwano, H Niwano, J Oikawa, H Nakamura, T Hashikata, T Igarashi, T Fujiishi, T Yoshizawa, J Kishihara, A Satoh, H Fukaya, J Ako |
International Heart Journal | 2019 |
Membrane Protein Complexes: Structure and Function
JR Harris, EJ Boekema |
2018 | |
Membrane Protein Complexes: Structure and Function
JR Harris, EJ Boekema |
2018 | |
Membrane Protein Complexes: Structure and Function
JR Harris, EJ Boekema |
2018 | |
Membrane Protein Complexes: Structure and Function
JR Harris, EJ Boekema |
2018 | |
Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure
A Kushnir, G Santulli, SR Reiken, E Coromilas, SJ Godfrey, DL Brunjes, PC Colombo, M Yuzefpolskaya, SI Sokol, RN Kitsis, AR Marks |
Circulation | 2018 |
Ryanodine receptor dysfunction in human disorders
A Kushnir, B Wajsberg, AR Marks |
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2018 |
Cardiac voltage gated calcium channels and their regulation by β-adrenergic signaling
N Kumari, H Gaur, A Bhargava |
Life Sciences | 2018 |
Sulforaphane potentiates anticancer effects of doxorubicin and attenuates its cardiotoxicity in a breast cancer model
C Bose, S Awasthi, R Sharma, H Beneš, M Hauer-Jensen, M Boerma, SP Singh, P Mukhopadhyay |
PloS one | 2018 |
Intracellular calcium release channels: an update: RyRs vs . IP 3 Rs
G Santulli, R Nakashima, Q Yuan, AR Marks |
The Journal of Physiology | 2017 |
Physiology and pathophysiology of excitation–contraction coupling: the functional role of ryanodine receptor
G Santulli, DR Lewis, AR Marks |
Journal of Muscle Research and Cell Motility | 2017 |
Intracellular calcium release channels: an update: RyRs vs . IP 3 Rs
G Santulli, R Nakashima, Q Yuan, AR Marks |
The Journal of Physiology | 2017 |
NFATc4 and myocardin synergistically up-regulate the expression of LTCC α1C in ET-1-induced cardiomyocyte hypertrophy
M Li, HP He, HQ Gong, J Zhang, W Ma, H Zhou, DS Cao, N Wang, TC Zhang |
Life Sciences | 2016 |
Effects of Dantrolene Treatment on Ventricular Electrophysiology and Arrhythmogenesis in Rats With Chronic β-Adrenergic Receptor Activation
T Liu, S Shi, M Qin, C Huang |
Journal of cardiovascular pharmacology and therapeutics | 2015 |
Thyroid hormone action in postnatal heart development
M Li, SE Iismaa, N Naqvi, A Nicks, A Husain, RM Graham |
Stem Cell Research | 2014 |
The soluble guanylyl cyclase activator bay 58-2667 selectively limits cardiomyocyte hypertrophy
JC Irvine, V Ganthavee, JE Love, AE Alexander, JD Horowitz, JP Stasch, BK Kemp-Harper, RH Ritchie |
PloS one | 2012 |
Pharmacoresistant Ca v 2·3 (E-type/R-type) voltage-gated calcium channels influence heart rate dynamics and may contribute to cardiac impulse conduction: Ca v 2·3 CALCIUM CHANNELS CONTRIBUTE TO CARDIAC REGULATION
T Galetin, EE Tevoufouet, J Sandmeyer, J Matthes, F Nguemo, J Hescheler, M Weiergräber, T Schneider |
Cell Biochemistry and Function | 2012 |
L-type Calcium Channel Auto-Regulation of Transcription
J Satin, EA Schroder, SM Crump |
Cell Calcium | 2011 |
Posttranscriptional Regulation of the β2-Subunit of Cardiac L-type Ca2+ Channels by MicroRNAs During Long-term Exposure to Isoproterenol in Rats :
ED Carrillo, Y Escobar, G González, A Hernández, JM Galindo, MC García, JA Sánchez |
Journal of Cardiovascular Pharmacology | 2011 |
Autoregulation of Cardiac l-Type Calcium Channels
J Satin, EA Schroder |
Trends in Cardiovascular Medicine | 2009 |
Calsequestrin isoforms localize to different ER subcompartments: Evidence for polymer and heteropolymer-dependent localization
ML Milstein, TD Houle, SE Cala |
Experimental Cell Research | 2009 |
L-Type Calcium Channel C Terminus Autoregulates Transcription
E Schroder, M Byse, J Satin |
Circulation research | 2009 |
Potential relevance of alpha(1)-adrenergic receptor autoantibodies in refractory hypertension
K Wenzel, H Haase, G Wallukat, W Derer, S Bartel, V Homuth, F Herse, N Hubner, H Schulz, M Janczikowski, C Lindschau, C Schroeder, S Verlohren, I Morano, DN Muller, FC Luft, R Dietz, R Dechend, P Karczewski |
PloS one | 2008 |
Effects of angiotensin II blockade on inflammation-induced alterations of pharmacokinetics and pharmacodynamics of calcium channel blockers
S Hanafy, NJ Dagenais, WF Dryden, F Jamali |
British Journal of Pharmacology | 2008 |
Effets pharmacologiques de Ziziphus mauritiana Lam. (Rhamnaceae) sur le cœur isolé et l’aorte de mammifères
F Traore, AL Adjoungoua, A Koffi, F Diafouka |
Phytothérapie | 2008 |
Design of Mutant 2 Subunits as Decoy Molecules to Reduce the Expression of Functional Ca2+ Channels in Cardiac Cells
S Telemaque, S Sonkusare, T Grain, SW Rhee, JR Stimers, NJ Rusch, JD Marsh |
The Journal of pharmacology and experimental therapeutics | 2008 |
Potential Relevance of α1-Adrenergic Receptor Autoantibodies in Refractory Hypertension
K Wenzel, H Haase, G Wallukat, W Derer, S Bartel, V Homuth, F Herse, N Hubner, H Schulz, M Janczikowski, C Lindschau, C Schroeder, S Verlohren, I Morano, DN Muller, FC Luft, R Dietz, R Dechend, P Karczewski, T Secomb |
PloS one | 2008 |
Adrenergic regulation of conduction velocity in cultures of immature cardiomyocytes
TP de Boer, HV van Rijen, MA van der Heyden, JM de Bakker, TA van Veen |
Netherlands Heart Journal | 2008 |
Beta-, Not Alpha-Adrenergic Stimulation Enhances Conduction Velocity in Cultures of Neonatal Cardiomyocytes
TP de Boer, HV van Rijen, MA van der Heyden, B Kok, T Opthof, MA Vos, HJ Jongsma, JM de Bakker, TA van Veen |
Circulation Journal | 2007 |
The role of sympathetic innervation in the developing rat gubernaculum
Y Sasaki, JM Hutson, MC Clarke |
Journal of Pediatric Surgery | 2007 |
Inefficient Glycosylation Leads to High Steady-state Levels of Actively Degrading Cardiac Triadin-1
ML Milstein, TP McFarland, JD Marsh, SE Cala |
The Journal of biological chemistry | 2007 |
Sustained β-Adrenergic Stimulation Increased L-Type Ca2+ Channel Expression in Cultured Quiescent Ventricular Myocytes
M Akuzawa-Tateyama, M Tateyama, R Ochi |
The Journal of Physiological Sciences | 2006 |
Vascular calcium channels and high blood pressure: Pathophysiology and therapeutic implications
S Sonkusare, PT Palade, JD Marsh, S Telemaque, A Pesic, NJ Rusch |
Vascular Pharmacology | 2006 |
Contractility of rat gubernacula affected by calcitonin gene-related peptide and β-agonist
H Tomiyama, JM Hutson |
Journal of Pediatric Surgery | 2005 |
L-type Ca2+ channels mediate adaptation of extracellular signal-regulated kinase 1/2 phosphorylation in the ventral tegmental area after chronic amphetamine treatment
A Rajadhyaksha, I Husson, SS Satpute, KD Küppenbender, JQ Ren, RM Guerriero, DG Standaert, BE Kosofsky |
The Journal of neuroscience : the official journal of the Society for Neuroscience | 2004 |
Tissue-specific expression of two human Cav1.2 isoforms under the control of distinct 5′ flanking regulatory elements
L Pang, G Koren, Z Wang, S Nattel |
FEBS Letters | 2003 |
Chemical sympathectomy by 6-OH dopamine during fetal life results in inguinal testis through altering cremasteric contractility in rats
FC Tanyel, M Ertunç, S Ekinci, S Otçu, M Yıldırım, R Onur |
Journal of Pediatric Surgery | 2003 |
Neuropeptide Y: Neurotransmitter or Trophic Factor in the Heart?
L Protas, J Qu, RB Robinson |
Physiology (Bethesda, Md.) | 2003 |
CARDIACIONCHANNELS
DM Roden, JR Balser, AL George, ME Anderson |
Annual Review of Physiology | 2002 |
Pharmacological support for children with myocardial dysfunction
P Booker |
Pediatric Anesthesia | 2002 |
Culture Environments
LE Freed, G Vunjak-Novakovic |
2002 | |
A 27 bp cis-acting sequence is essential for L-type calcium channel α1C subunit expression in vascular smooth muscle cells
QI Fan, K Vanderpool, JD Marsh |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 2002 |
Effects of sustained β-adrenergic stimulation on ionic currents of cultured adult guinea pig cardiomyocytes
LM Zhang, Z Wang, S Nattel |
American journal of physiology. Heart and circulatory physiology | 2002 |
Exercise training normalizes altered calcium-handling proteins during development of heart failure
L Lu, DF Mei, AG Gu, S Wang, B Lentzner, DE Gutstein, D Zwas, S Homma, GH Yi, J Wang |
Journal of applied physiology | 2002 |
Upregulation of L-Type Ca 2+ Channels in Mesenteric and Skeletal Arteries of SHR
PF Pratt, S Bonnet, LM Ludwig, P Bonnet, NJ Rusch |
Hypertension | 2002 |
Structure-Functional Diversity of Human L-Type Ca 2+ Channel: Perspectives for New Pharmacological Targets: Figure 1
DR Abernethy, NM Soldatov |
The Journal of pharmacology and experimental therapeutics | 2002 |
Norepinephrine regulates the in vivo expression of the L-type calcium channel
Kish L Golden, Jun Ren, Andrea Dean, James D Marsh |
Molecular and Cellular Biochemistry | 2002 |
Down-regulation of L-type calcium channels in inflamed circular smooth muscle cells of the canine colon
X Liu, NJ Rusch, J Striessnig, SK Sarna |
Gastroenterology | 2001 |
Mechanisms involved in contractile differences among cremaster muscles according to localization of testis
FC Tanyel, M Ertunç, N Büyükpamukçu, R Onur |
Journal of Pediatric Surgery | 2001 |
Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies
M Papadaki, N Bursac, R Langer, J Merok, G Vunjak-Novakovic, LE Freed |
American journal of physiology. Heart and circulatory physiology | 2001 |
In vivo regulation of Na/Ca exchanger expression by adrenergic effectors
KL Golden, J Ren, J O'Connor, A Dean, SE DiCarlo, JD Marsh |
American journal of physiology. Heart and circulatory physiology | 2001 |
Ras Reduces L-Type Calcium Channel Current in Cardiac Myocytes: Corrective Effects of L-Channels and SERCA2 on [Ca 2+ ] i Regulation and Cell Morphology
PD Ho, JS Fan, NL Hayes, N Saada, PT Palade, CC Glembotski, PM McDonough |
Circulation research | 2001 |
Transcriptional Regulation of L-type Calcium Channel Expression in Cardiac Myocytes
IQ Fan, B Chen, JD Marsh |
Journal of Molecular and Cellular Cardiology | 2000 |
Adrenergic Stimulation Regulates Na+/Ca2+Exchanger Expression in Rat Cardiac Myocytes
KL Golden, QI Fan, B Chen, J Ren, J O»Connor, JD Marsh |
Journal of Molecular and Cellular Cardiology | 2000 |
Regulation of DHP receptor expression by elements in the 5′-flanking sequence
L Liu, QI Fan, MR El-Zaru, K Vanderpool, RN Hines, JD Marsh |
American journal of physiology. Heart and circulatory physiology | 2000 |
Hormonal control of cardiac ion channels and transporters
Y Shimoni |
Progress in Biophysics and Molecular Biology | 1999 |
β 2 -Adrenergic receptor overexpression in the developing mouse heart: evidence for targeted modulation of ion channels
RH An, BM Heath, JP Higgins, WJ Koch, RJ Lefkowitz, RS Kass |
The Journal of Physiology | 1999 |
Neuropeptide Y contributes to innervation-dependent increase in ICa,L via ventricular Y 2 receptors
L Protas, RB Robinson |
American journal of physiology. Heart and circulatory physiology | 1999 |
Effects of Adrenergic Stimulation on Postnatal Development and Calcium Current in Newborn Rat Cardiomyocytes in Primary Culture
C Pignier, N Fares, D Potreau |
Journal of Cardiovascular Pharmacology | 1998 |
Retinoic Acid Induction of Calcium Channel Expression in Human NT2N Neurons
Z Gao, G Xu, MM Stwora-Wojczyk, FM Matschinsky, VM Lee, BA Wolf |
Biochemical and Biophysical Research Communications | 1998 |
Electrophysiological properties of neonatal rat ventricular myocytes with α 1 -adrenergic-induced hypertrophy
JP Gaughan, CA Hefner, SR Houser |
American journal of physiology. Heart and circulatory physiology | 1998 |
Overexpression of α 1B -adrenergic receptor induces left ventricular dysfunction in the absence of hypertrophy
IL Grupp, JN Lorenz, RA Walsh, GP Boivin, H Rindt |
American journal of physiology. Heart and circulatory physiology | 1998 |
L-type calcium channel expression depends on the differentiated state of vascular smooth muscle cells
M Gollasch, H Haase, C Ried, C Lindschau, I Morano, FC Luft, H Haller |
The FASEB Journal | 1998 |
Androgen Receptors Mediate Hypertrophy in Cardiac Myocytes
JD Marsh, MH Lehmann, RH Ritchie, JK Gwathmey, GE Green, RJ Schiebinger |
Circulation | 1998 |
Increased Availability and Open Probability of Single L-Type Calcium Channels From Failing Compared With Nonfailing Human Ventricle
F Schröder, R Handrock, DJ Beuckelmann, S Hirt, R Hullin, L Priebe, RH Schwinger, J Weil, S Herzig |
Circulation | 1998 |
Bradykinin Blocks Angiotensin II–Induced Hypertrophy in the Presence of Endothelial Cells
RH Ritchie, JD Marsh, WD Lancaster, CA Diglio, RJ Schiebinger |
Hypertension | 1998 |
Role of Renal Nerves in Afferent Arteriolar Reactivity in Angiotensin-Induced Hypertension
A Ichihara, EW Inscho, JD Imig, RE Michel, LG Navar |
Hypertension | 1997 |