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Citations to this article

A new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization.
N Busch, … , H U Marschall, S Matern
N Busch, … , H U Marschall, S Matern
Published December 1, 1995
Citation Information: J Clin Invest. 1995;96(6):3009-3015. https://doi.org/10.1172/JCI118374.
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Research Article

A new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization.

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Abstract

Biliary proteins inhibiting or promoting cholesterol crystallization are assumed to play a major role in cholesterol gallstone pathogenesis. We now report a new group of biliary proteins that bind to cholesterol crystals, modify crystal morphology, and inhibit cholesterol crystallization. Various glycoprotein mixtures were extracted from abnormal human gallbladder bile using lectin affinity chromatography on concanavalin A, lentil, and Helix pomatia columns and were added to supersaturated model bile. Independent of the protein mixtures added, from the cholesterol crystals harvested, the same four GPs were isolated having molecular masses of 16, 28, 63, and 74 kD, respectively. Each protein was purified using preparative SDS-PAGE, and influence on cholesterol crystallization in model bile was tested at 10 microg/ml. Crystal growth was reduced by 76% (GP63), 65% (GP16), 55% (GP74), and 40% (GP28), respectively. Thus, these glycoproteins are the most potent biliary inhibitors of cholesterol crystallization known so far. Evidence that the inhibiting effect on cholesterol crystallization is mediated via protein-crystal interaction was further provided from scanning electron microscopy studies. Crystals grown in presence of inhibiting proteins showed significantly more ordered structures. Incidence of triclinic crystals and regular aggregates was shifted from 30 to 70% compared with controls. These observations may have important implications for understanding the role of biliary proteins in cholesterol crystallization and gallstone pathogenesis.

Authors

N Busch, F Lammert, H U Marschall, S Matern

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Total citations by year

Year: 2019 2017 2007 2003 2002 2001 2000 1999 1998 1997 1996 1971 Total
Citations: 1 1 2 1 2 1 2 5 4 1 1 1 22
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2007 (2)

Title and authors Publication Year
Gallstone disease
HU Marschall, C Einarsson
Journal of Internal Medicine 2007
Genetic evidence that apolipoprotein E4 is not a relevant susceptibility factor for cholelithiasis in two high-risk populations
JG Mella, R Schirin-Sokhan, A Rigotti, F Pimentel, L Villarroel, HE Wasmuth, T Sauerbruch, F Nervi, F Lammert, JF Miquel
Journal of lipid research 2007

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