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Citations to this article

Expression and localization of inducible and endothelial nitric oxide synthase in the rat ovary. Effects of gonadotropin stimulation in vivo.
B J Van Voorhis, … , D Grzybicki, C P Weiner
B J Van Voorhis, … , D Grzybicki, C P Weiner
Published December 1, 1995
Citation Information: J Clin Invest. 1995;96(6):2719-2726. https://doi.org/10.1172/JCI118339.
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Research Article

Expression and localization of inducible and endothelial nitric oxide synthase in the rat ovary. Effects of gonadotropin stimulation in vivo.

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Abstract

Nitric oxide is reportedly involved in the regulation of several ovarian processes, yet the isoforms of nitric oxide synthase (NOS) expressed in the ovary are unknown. Our purpose was to identify and localize NOS isoenzymes in the rat ovary and to examine++ if mRNA expression of NOS isoenzymes change after gonadotropin stimulation. Using reverse transcriptase-PCR, we demonstrated that inducible (iNOS) and endothelial (eNOS), but not neuronal, NOS mRNAs are expressed in the ovary. In a gonadotropin-stimulated rat model, unstimulated ovaries had the highest levels of iNOS mRNA as quantified by ribonuclease protection assay. After gonadotropin injection, iNOS mRNA declined to undetectable levels in ovaries containing ovulatory follicles before increasing slighty in ovaries containing copora lutea. In situ hybridization studies localized iNOS to granulosa cells of secondary follicles and small antral follicles. Western blots of unstimulated ovaries demonstrated iNOS protein. In contrast to iNOS, eNOS mRNA levels, determined by quantitative PCR, increased after gonadotropin stimulation and peaked in ovaries containing ovulatory follicles before declining in the luteal phase. eNOS protein was localized to blood vessels in the ovary by immunohistochemistry. We conclude that two isoforms of NOS are expressed in the ovary and the mRNA levels for these isozymes are differentially regulated.

Authors

B J Van Voorhis, K Moore, P J Strijbos, S Nelson, S A Baylis, D Grzybicki, C P Weiner

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Total citations by year

Year: 2023 2021 2019 2018 2017 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1983 Total
Citations: 1 2 3 1 1 2 1 3 3 2 2 1 3 2 2 5 6 10 6 11 6 1 7 1 82
Citation information
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Citations to this article in year 2010 (2)

Title and authors Publication Year
Nitric oxide synthase isoforms and the effect of their inhibition on meiotic maturation of porcine oocytes
E Chmelíková, M Ješeta, M Sedmíková, J Petr, L Tůmová, T Kott, P Lipovová, F Jílek
Zygote 2010
Reactive oxygen and nitrogen species during meiotic resumption from diplotene arrest in mammalian oocytes
AN Pandey, A Tripathi, KV PremKumar, TG Shrivastav, SK Chaube
Journal of Cellular Biochemistry 2010

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