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Citations to this article

Ischemic preconditioning stimulates sodium and proton transport in isolated rat hearts.
R Ramasamy, … , J Lundmark, S Schaefer
R Ramasamy, … , J Lundmark, S Schaefer
Published September 1, 1995
Citation Information: J Clin Invest. 1995;96(3):1464-1472. https://doi.org/10.1172/JCI118183.
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Research Article

Ischemic preconditioning stimulates sodium and proton transport in isolated rat hearts.

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Abstract

One or more brief periods of ischemia, termed preconditioning, dramatically limits infarct size and reduces intracellular acidosis during subsequent ischemia, potentially via enhanced sarcolemmal proton efflux mechanisms. To test the hypothesis that preconditioning increases the functional activity of sodium-dependent proton efflux pathways, isolated rat hearts were subjected to 30 min of global ischemia with or without preconditioning. Intracellular sodium (Nai) was assessed using 23Na magnetic resonance spectroscopy, and the activity of the Na-H exchanger and Na-K-2Cl cotransporter was measured by transiently exposing the hearts to an acid load (NH4Cl washout). Creatine kinase release was reduced by greater than 60% in the preconditioned hearts (P < 0.05) and was associated with improved functional recovery on reperfusion. Preconditioning increased Nai by 6.24 +/- 2.04 U, resulting in a significantly higher level of Nai before ischemia than in the control hearts. Nai increased significantly at the onset of ischemia (8.48 +/- 1.21 vs. 2.57 +/- 0.81 U, preconditioned vs. control hearts; P < 0.01). Preconditioning did not reduce Nai accumulation during ischemia, but the decline in Nai during the first 5 min of reperfusion was significantly greater in the preconditioned than in the control hearts (13.48 +/- 1.73 vs. 2.54 +/- 0.41 U; P < 0.001). Exposure of preconditioned hearts to ethylisopropylamiloride or bumetanide in the last reperfusion period limited in the increase in Nai during ischemia and reduced the beneficial effects of preconditioning. After the NH4Cl prepulse, preconditioned hearts acidified significantly more than control hearts and had significantly more rapid recovery of pH (preconditioned, delta pH = 0.35 +/- 0.04 U over 5 min; control, delta pH = 0.15 +/- 0.02 U over 5 min). This rapid pH recovery was not affected by inhibition of the Na-K-2Cl cotransporter but was abolished by inhibition of the Na-H exchanger. These results demonstrate that preconditioning alters the kinetics of Nai accumulation during global ischemia as well as proton transport after NH4Cl washout. These observations are consistent with stimulation of the Na-K-2Cl cotransporter and Na-H exchanger by preconditioning.

Authors

R Ramasamy, H Liu, S Anderson, J Lundmark, S Schaefer

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Total citations by year

Year: 2019 2018 2015 2011 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 Total
Citations: 1 1 1 2 1 2 1 2 1 2 3 2 5 3 8 5 9 3 52
Citation information
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Citations to this article (52)

Title and authors Publication Year
Tissue acidosis does not mediate the hypoxia selectivity of [64Cu][Cu(ATSM)] in the isolated perfused rat heart
F Baark, F Shaughnessy, VR Pell, JE Clark, TR Eykyn, P Blower, R Southworth
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The Role of Sodium in Diabetic Cardiomyopathy
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Frontiers in physiology 2018
Ionic homeostasis in brain conditioning
O Cuomo, A Vinciguerra, P Cerullo, S Anzilotti, P Brancaccio, L Bilo, A Scorziello, P Molinaro, GD Renzo, G Pignataro
Frontiers in neuroscience 2015
Mitochondrial Energetics, pH Regulation, and Ion Dynamics: A Computational-Experimental Approach
AC Wei, MA Aon, B O'Rourke, RL Winslow, S Cortassa
Biophysical Journal 2011
Phosphorylation of ARC Is a Critical Element in the Antiapoptotic Effect of Anesthetic Preconditioning
X Lu, PG Moore, H Liu, S Schaefer
Anesthesia & Analgesia 2011
Effect of sodium load of the matrix on properties of isolated rat heart mitochondria
SM Korotkov, VP Nesterov, IN Demina
Doklady Biochemistry and Biophysics 2009
Polyol pathway and modulation of ischemia-reperfusion injury in Type 2 diabetic BBZ rat hearts
Q Li, YC Hwang, R Ananthakrishnan, PJ Oates, D Guberski, R Ramasamy
Cardiovascular Diabetology 2008
Acidic Preconditioning Inhibits Na+/H+ and Na+/Ca2+ Exchanger Interaction via PKCε in Guinea-Pig Ventricular Myocytes
L Li, Y Watanabe, I Matsuoka, J Kimura
Journal of Pharmacological Sciences 2008
Effect of diazoxide and Ca2+ on rat heart mitochondria loaded with Na+
SM Korotkov, VP Nesterov, IN Demina, NN Ryabchikov
Doklady Biochemistry and Biophysics 2007
Increased expression and function of the myocardial Na–K–2Cl cotransporter in failing rat hearts
GØ Andersen, E Øie, LE Vinge, A Yndestad, H Attramadal, T Skomedal, JB Osnes
Basic Research in Cardiology 2006
Effects of cold cardioplegia on pH, Na, and Ca in newborn rabbit hearts
SE Anderson, H Liu, A Beyschau, PM Cala
American journal of physiology. Heart and circulatory physiology 2006
Acute effects of 17β-estradiol on myocardial pH, Na + , and Ca 2+ and ischemia-reperfusion injury
SE Anderson, DM Kirkland, A Beyschau, PM Cala
American journal of physiology. Cell physiology 2005
Reduced Efficacy of Volatile Anesthetic Preconditioning with Advanced Age in Isolated Rat Myocardium
R Sniecinski, H Liu
Anesthesiology 2004
Effects of nicorandil on cardiac sympathetic nerve activity after reperfusion therapy in patients with first anterior acute myocardial infarction
S Kasama, T Toyama, H Kumakura, Y Takayama, S Ichikawa, T Suzuki, M Kurabayashi
European Journal of Nuclear Medicine and Molecular Imaging 2004
Intra-myocyte ion homeostasis during ischemia-reperfusion injury: effects of pharmacologic preconditioning and controlled reperfusion
JE Davies, SB Digerness, SP Goldberg, CR Killingsworth, CR Katholi, PS Brookes, WL Holman
The Annals of Thoracic Surgery 2003
Myocardial Ischemia and Preconditioning
NS Dhalla, N Takeda, M Singh, A Lukas
2003
The Sodium-Hydrogen Exchanger
M Karmazyn, M Avkiran, L Fliegel
2003
Ischemic preconditioning and Na+/H+ exchange inhibition improve reperfusion ion homeostasis
SP Goldberg, SB Digerness, JL Skinner, CR Killingsworth, CR Katholi, WL Holman
The Annals of Thoracic Surgery 2002
Influence of Ischemic Preconditioning on Intracellular Sodium, pH, and Cellular Energy Status in Isolated Perfused Heart
A Babsky, S Hekmatyar, S Wehrli, N Doliba, M Osbakken, N Bansal
Experimental biology and medicine (Maywood, N.J.) 2002
The Effect of KATPChannel Activation on Myocardial Cationic and Energetic Status During Ischemia and Reperfusion: Role in Cardioprotection
H Fukuda, CS Luo, X Gu, LL Guo, SB Digerness, J Li, MM Pike
Journal of Molecular and Cellular Cardiology 2001
Protecting the Myocardium From Ischemic Injury
S Salvi
Chest 2001
Na + Effects on Mitochondrial Respiration and Oxidative Phosphorylation in Diabetic Hearts
A Babsky, N Doliba, N Doliba, A Savchenko, S Wehrli, M Osbakken
Experimental biology and medicine (Maywood, N.J.) 2001
Detection of myocardial viability based on measurement of sodium content: A23Na-NMR study
M Horn, C Weidensteiner, H Scheffer, M Meininger, M Groot, H Remkes, C Dienesch, K Przyklenk, M Kienlin, S Neubauer
Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine 2001
Role of Intracellular Na + Kinetics in Preconditioned Rat Heart
K Imahashi, T Nishimura, J Yoshioka, H Kusuoka
Circulation research 2001
Inhibitors of Ischemic Preconditioning Do Not Attenuate Na+/H+ Exchange Inhibitor Mediated Cardioprotection
RJ Gumina, N Beier, P Schelling, GJ Gross
Journal of Cardiovascular Pharmacology 2000
The role of adenosine in preconditioning
JW Jong, R Jonge, E Keijzer, S Bradamante
Pharmacology & Therapeutics 2000
Effect of ethylisopropyl amiloride, a Na+ - H+ exchange inhibitor, on cardioprotective effect of ischaemic and angiotensin preconditioning
A Sharma, M Singh
Molecular and Cellular Biochemistry 2000
Effects and interaction, of cariporide and preconditioning on cardiac arrhythmias and infarction in ratin vivo
NN Aye, S Komori, K Hashimoto
British Journal of Pharmacology 1999
Cellular Mechanisms of Infarct Size Reduction with Ischemic Preconditioning: Role of Calcium?
K Przyklenk, BZ Simkhovich, B Bauer, K Hata, L Zhao, GT Elliott, RA Kloner
Annals of the New York Academy of Sciences 1999
ADENOSINE AND PRECONDITIONING REVISITED
T Miura, A Tsuchida
Clinical and Experimental Pharmacology and Physiology 1999
Role of Ischemic Preconditioning on Ischemia-Reperfusion Injury of the Lung
H Soncul, E Oz, S Kalaycioglu
Chest 1999
Chelerythrine increases Na-K-ATPase activity and limits ischemic injury in isolated rat hearts
JL Lundmark, R Ramasamy, PR Vulliet, S Schaefer
American journal of physiology. Heart and circulatory physiology 1999
Inhibition of the Na + /H + Exchanger Confers Greater Cardioprotection Against 90 Minutes of Myocardial Ischemia Than Ischemic Preconditioning in Dogs
RJ Gumina, E Buerger, C Eickmeier, J Moore, J Daemmgen, GJ Gross
Circulation 1999
Protection of the Myocardium During Ischemia and Reperfusion: Na + /H + Exchange Inhibition Versus Ischemic Preconditioning
M Avkiran
Circulation 1999
If ischemic preconditioning is the gold standard, has a platinum standard of cardioprotection arrived? Comparison with NHE inhibition
R J Gumina, G J Gross
Journal of Thrombosis and Thrombolysis 1999
Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart
VD Schepkin, IO Choy, TF Budinger, DY Obayashi, SE Taylor, WM Decampli, SC Amartur, JN Young
Magnetic Resonance in Medicine 1998
Mechanisms of pH preservation during global ischemia in preconditioned rat heart: roles for PKC and NHE
TF Rehring, JI Shapiro, BS Cain, DR Meldrum, JC Cleveland, AH Harken, A Banerjee
American journal of physiology. Heart and circulatory physiology 1998
Metabolic effects of aldose reductase inhibition during low-flow ischemia and reperfusion
R Ramasamy, N Trueblood, S Schaefer
American journal of physiology. Heart and circulatory physiology 1998
Metabolic inhibition in the perfused rat heart: evidence for glycolytic requirement for normal sodium homeostasis
J Dizon, D Burkhoff, J Tauskela, J Whang, P Cannon, J Katz
American journal of physiology. Heart and circulatory physiology 1998
Protection Against Ischemia/Reperfusion Damage of the Heart
Y Abiko, M Karmazyn
1998
Infarct Size Limitation by a New Na-H Exchange Inhibitor, Hoe 642: Difference From Preconditioning in the Role of Protein Kinase C
T Miura, T Ogawa, K Suzuki, M Goto, K Shimamoto
Journal of the American College of Cardiology 1997
Evaluation of Multiple-Quantum-Filtered23Na NMR in Monitoring Intracellular Na Content in the Isolated Perfused Rat Heart in the Absence of a Chemical-Shift Reagent
JS Tauskela, JM Dizon, J Whang, J Katz
Journal of Magnetic Resonance 1997
Electrophysiological approach of the role of Na + /H + exchange in low-flow global ischemia and in ischemic preconditioning
L Perchenet, A Rochetaing, Y Gallois, P Kreher
Canadian Journal of Physiology and Pharmacology 1997
Ischemic preconditioning triggers phospholipase D signaling in rat heart
A Tosaki, N Maulik, G Cordis, OC Trifan, LM Popescu, DK Das
American journal of physiology. Heart and circulatory physiology 1997
The Na–H exchanger revisited: an update on Na–H exchange regulation and the role of the exchanger in hypertension and cardiac function in health and disease
O Fröhlich, M Karmazyn
Cardiovascular Research 1997
Decreased intracellular pH is not due to increased H + extrusion in preconditioned rat hearts
SA Gabel, HR Cross, RE London, C Steenbergen, E Murphy
American journal of physiology. Heart and circulatory physiology 1997
Is Calcium a Mediator of Infarct Size Reduction With Preconditioning in Canine Myocardium?
K Przyklenk, K Hata, RA Kloner
Circulation 1997
Na + /H + Exchanger Activity Does Not Contribute to Protection by Ischemic Preconditioning in the Isolated Rat Heart
AR Shipolini, H Yokoyama, M Galiñanes, SJ Edmondson, DJ Hearse, M Avkiran
Circulation 1997
The mechanism of protection from 5 (N-ethyl-N-isopropyl)amiloride differs from that of ischemic preconditioning in rabbit heart
H Sato, T Miki, RP Vallabhapurapu, P Wang, , MV Cohen, JM Downey
Basic Research in Cardiology 1997
Sodium alterations in isolated rat heart during cardioplegic arrest
VD Schepkin, IO Choy, TF Budinger
Journal of applied physiology 1996
New Paradigms of Coronary Artery Disease
G Heusch, R Schulz
1996
Preconditioning--a reappraisal of protection
L M King, L H Opie
Basic Research in Cardiology 1996

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