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Citations to this article

Effect of glucagon on intracellular pH regulation in isolated rat hepatocyte couplets.
D Alvaro, … , C Piat, L Capocaccia
D Alvaro, … , C Piat, L Capocaccia
Published August 1, 1995
Citation Information: J Clin Invest. 1995;96(2):665-675. https://doi.org/10.1172/JCI118109.
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Research Article

Effect of glucagon on intracellular pH regulation in isolated rat hepatocyte couplets.

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Abstract

To elucidate mechanisms of glucagon-induced bicarbonate-rich choleresis, we investigated the effect of glucagon on ion transport processes involved in the regulation of intracellular pH (pHi) in isolated rat hepatocyte couplets. It was found that glucagon (200 nM), without influencing resting pHi, significantly stimulates the Cl-/HCO3- exchange activity. The effect of glucagon was associated with a sevenfold increase in cAMP levels in rat hepatocytes. The activity of the Cl-/HCO3- exchanger was also stimulated by DBcAMP + forskolin. The effect of glucagon on the Cl-/HCO3- exchange was individually blocked by two specific and selective inhibitors of protein kinase A, Rp-cAMPs (10 microM) and H-89 (30 microM), the latter having no influence on the glucagon-induced cAMP accumulation in isolated rat hepatocytes. The Cl- channel blocker, NPPB (10 microM), showed no effect on either the basal or the glucagon-stimulated Cl-/HCO3 exchange. In contrast, the protein kinase C agonist, PMA (10 microM), completely blocked the glucagon stimulation of the Cl-/HCO3- exchange; however, this effect was achieved through a significant inhibition of the glucagon-stimulated cAMP accumulation in rat hepatocytes. Colchicine pretreatment inhibited the basal as well as the glucagon-stimulated Cl-/HCO3- exchange activity. The Na+/H+ exchanger was unaffected by glucagon either at basal pHi or at acid pHi values. In contrast, glucagon, at basal pHi, stimulated the Na(+)-HCO3- symport. The main findings of this study indicate that glucagon, through the cAMP-dependent protein kinase A pathway, stimulates the activity of the Cl-/HCO3- exchanger in isolated rat hepatocyte couplets, a mechanism which could account for the in vivo induced bicarbonate-rich choleresis.

Authors

D Alvaro, P Della Guardia, A Bini, A Gigliozzi, S Furfaro, T La Rosa, C Piat, L Capocaccia

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Total citations by year

Year: 2020 2019 2015 2014 2013 2011 2008 2006 2005 2004 2003 2002 2000 1999 1998 1997 Total
Citations: 1 3 2 1 3 1 3 3 3 2 2 2 3 1 2 3 35
Citation information
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Citations to this article in year 2011 (1)

Title and authors Publication Year
Tauroursodeoxycholate (TUDCA) inhibits neointimal hyperplasia by suppression of ERK via PKC -mediated MKP-1 induction
SY Kim, YW Kwon, IL Jung, JH Sung, SG Park
Cardiovascular Research 2011

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