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Citations to this article

Nonenzymatic glycosylation in vitro and in bovine endothelial cells alters basic fibroblast growth factor activity. A model for intracellular glycosylation in diabetes.
I Giardino, … , D Edelstein, M Brownlee
I Giardino, … , D Edelstein, M Brownlee
Published July 1, 1994
Citation Information: J Clin Invest. 1994;94(1):110-117. https://doi.org/10.1172/JCI117296.
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Research Article Article has an altmetric score of 9

Nonenzymatic glycosylation in vitro and in bovine endothelial cells alters basic fibroblast growth factor activity. A model for intracellular glycosylation in diabetes.

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Authors

I Giardino, D Edelstein, M Brownlee

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E Beltramo, E Berrone, S Tarallo, M Porta
Acta Diabetologica 2008
Advanced glycation end products and diabetic foot disease
MS Huijberts, NC Schaper, CG Schalkwijk
Diabetes/Metabolism Research and Reviews 2008
Skin autofluorescence is increased in systemic lupus erythematosus but is not reflected by elevated plasma levels of advanced glycation endproducts
HL Nienhuis, K Leeuw, J Bijzet, A Smit, CG Schalkwijk, R Graaff, CG Kallenberg, M Bijl
Rheumatology 2008
Diabetes-related microvascular and macrovascular diseases in the physical therapy setting
WT Cade
Physical therapy 2008
Psychoneuroimmunology
F Tausk, I Elenkov, J Moynihan
Dermatologic Therapy 2008
Series in Medical Physics and Biomedical Engineering: Physiological Norm and Pathology
T Denisova, L Malinova
Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging 2008
Fructose-induced structural and functional modifications of hemoglobin: Implication for oxidative stress in diabetes mellitus
T Bose, AS Chakraborti
Biochimica et Biophysica Acta (BBA) - General Subjects 2008
Action of pelargonidin on hyperglycemia and oxidative damage in diabetic rats: Implication for glycation-induced hemoglobin modification
M Roy, S Sen, AS Chakraborti
Life Sciences 2008
Controversies in Treating Diabetes
D LeRoith, AI Vinik
2008
Oxidative Stress in Aging
S Miwa, KB Beckman, FL Muller
2008
Inhibition of Advanced Glycation and Absence of Galectin-3 Prevent Blood-Retinal Barrier Dysfunction during Short-Term Diabetes
P Canning, JV Glenn, DK Hsu, FT Liu, TA Gardiner, AW Stitt
Experimental Diabetes Research 2007
Cardiovascular Regeneration and Stem Cell Therapy
K Mandal, M Jahangiri, Q Xu
Cardiovascular Regeneration and Stem Cell Therapy 2007
Mechanisms of Disease: endothelial dysfunction in insulin resistance and diabetes
C Rask-Madsen, GL King
Nature Clinical Practice Endocrinology & Metabolism 2007
Identification of Antidiabetic Effect of Iridoid Glycosides and Low Molecular Weight Polyphenol Fractions of Corni Fructus, a Constituent of Hachimi-jio-gan, in Streptozotocin-Induced Diabetic Rats
N Yamabe, KS Kang, Y Matsuo, T Tanaka, T Yokozawa
Biological & Pharmaceutical Bulletin 2007
Diabetes and Endothelial Dysfunction
M Schiavoni, F Cosentino, GG Camici, TF Luescher
High Blood Pressure & Cardiovascular Prevention 2007
Oxidative stress, AGE, and atherosclerosis
E Schleicher, U Friess
Kidney International 2007
Dihydroxyacetone-induced death is accompanied by advanced glycation endproduct formation in selected proteins ofSaccharomyces cerevisiae andCaenorhabditis elegans
M Molin, M Pilon, A Blomberg
PROTEOMICS 2007
How to design a biosensor
WK Ward
Journal of diabetes science and technology 2007
Method for chronological recording of antigen appearance in human head-hair shafts and its use for monitoring glycation products in diabetes
J Nissimov, U Elchalal, H Bakala, M Brownlee, E Berry, M Phillip, Y Milner
Journal of Immunological Methods 2007
Diabetes and Endothelial Dysfunction: What???s the Culprit?
M Schiavoni, F Cosentino, GG Camici, TF Luescher
High Blood Pressure & Cardiovascular Prevention 2007
Analysis of DNA-bound advanced glycation end-products by LC and mass spectrometry
C Bidmon, M Frischmann, M Pischetsrieder
Journal of Chromatography B 2007
Comprehensive Glycoscience
S Kelm, R Ravindran
Comprehensive Glycoscience 2007
Therapeutic Neovascularization–Quo Vadis?
E Deindl, C Kupatt
2007
A large-scale proteomic analysis of human embryonic stem cells
TC Schulz, AM Swistowska, Y Liu, A Swistowski, G Palmarini, SN Brimble, E Sherrer, AJ Robins, MS Rao, X Zeng
BMC Genomics 2007
Benfotiamine accelerates the healing of ischaemic diabetic limbs in mice through protein kinase B/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis
S Gadau, C Emanueli, S Linthout, G Graiani, M Todaro, M Meloni, I Campesi, G Invernici, F Spillmann, K Ward, P Madeddu
Diabetologia 2006
Diabetes and Advanced Glycoxidation End Products
AG Huebschmann, JG Regensteiner, H Vlassara, JE Reusch
Diabetes care 2006
Identification of AGE-modified proteins in SH-SY5Y and OLN-93 Cells
AÉ Langer, HF Poon, G Münch, BC Lynn, T Arendt, DA Butterfield
Neurotoxicity Research 2006
Air pollution-associated fly ash particles induce fibrotic mechanisms in primary fibroblasts
T Gursinsky, S Ruhs, U Friess, S Diabaté, HF Krug, RE Silber, A Simm
Biological Chemistry 2006
Effects of Low-Carbohydrate Diet and Pycnogenol®Treatment on Retinal Antioxidant Enzymes in Normal and Diabetic Rats
ZT Kamuren, CG Mcpeek, RA Sanders, JB Watkins
Journal of Ocular Pharmacology and Therapeutics 2006
Heat-shock protein 27 is a major methylglyoxal-modified protein in endothelial cells
CG Schalkwijk, J Bezu, RC van der Schors, K Uchida, CD Stehouwer, VW van Hinsbergh
FEBS Letters 2006
Comparison of protective effects of aspirin, d-penicillamine and vitamin E against high glucose-mediated toxicity in cultured endothelial cells
J Zhang, M Slevin, Y Duraisamy, J Gaffney, CA Smith, N Ahmed
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2006
Early glycation products of endothelial plasma membrane proteins in experimental diabetes
S Nguyen, M Pascariu, L Ghitescu
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2006
Directionally Guided Angiogenesis Within Extruded Type I Collagen Filaments In Vivo
DP Speer, JB Ulreich, NJ Young, D Yuen, ST Li
Journal of Histotechnology 2006
Glycation and Advanced Glycation End-Products in Laboratory Experiments in Vivo and in Vitro
M Beránek, D Nováková, P Rozsíval, J Dršata, V Palička
Acta Medica (Hradec Kralove, Czech Republic) 2006
Molekularmedizinische Grundlagen von para- und autokrinen Regulationsstörungen
D Ganten, K Ruckpaul, J Köhrle
2006
High glucose inhibits human epidermal keratinocyte proliferation for cellular studies on diabetes mellitus
H Terashi, K Izumi, M Deveci, LM Rhodes, CL Marcelo
International Wound Journal 2005
Glycation-induced inactivation of aspartate aminotransferase, effect of uric acid
I Boušová, H Bakala, R Chudáček, V Palička, J Dršata
Molecular and Cellular Biochemistry 2005
Endothelial cell injury by high glucose and heparanase is prevented by insulin, heparin and basic fibroblast growth factor
J Han, AK Mandal, LM Hiebert
Cardiovascular Diabetology 2005
Glyco-oxidation in diabetes and related diseases
A Lapolla, D Fedele, P Traldi
Clinica Chimica Acta 2005
Effect of nonenzymatic glycation on functional and structural properties of hemoglobin
S Sen, M Kar, A Roy, AS Chakraborti
Biophysical Chemistry 2005
Advanced glycation endproducts—role in pathology of diabetic complications
N Ahmed
Diabetes Research and Clinical Practice 2005
Localization of the Ezrin Binding Epitope for Glycated Proteins
EA McROBERT, A Tikoo, MA Gallicchio, ME Cooper, LA Bach
Annals of the New York Academy of Sciences 2005
Effects of Advanced Glycation End Products on Ezrin-Dependent Functions in LLC-PK1 Proximal Tubule Cells
LA Bach, MA Gallicchio, EA McROBERT, A Tikoo, ME Cooper
Annals of the New York Academy of Sciences 2005
Xylosyltransferase I acceptor properties of fibroblast growth factor and its fragment bFGF (1–24)
J Kuhn, M Schnölzer, S Schön, S Müller, C Prante, C Götting, K Kleesiek
Biochemical and Biophysical Research Communications 2005
Collagen Ultrastructure and TGF-β1 Expression Preserved with Aminoguanidine During Wound Healing in Diabetic Rats
D Yavuz, H Tuğtepe, Ş Çetinel, S Uyar, H Kaya, G Haklar, S Civelek, O Deyneli, T Şan, G Burçak, S Akalin
Endocrine Research 2005
Advanced glycation end products and the kidney
JM Bohlender, S Franke, G Stein, G Wolf
American journal of physiology. Renal physiology 2005
Diabetes und Herz
T Meinertz, P Rösen, D Ziegler, A Schömig, D Tschöpe
2005
Substrates modified by advanced glycation end-products cause dysfunction and death in retinal pericytes by reducing survival signals mediated by platelet-derived growth factor
AW Stitt, SJ Hughes, P Canning, O Lynch, O Cox, N Frizzell, SR Thorpe, TG Cotter, TM Curtis, TA Gardiner
Diabetologia 2004
Impact of in vivo glycation of LDL on platelet aggregation and monocyte chemotaxis in diabetic Psammomys obesus
M Zoltowska, E Delvin, E Ziv, N Peretti, M Chartré, E Levy
Lipids 2004
Cancer in south Karnataka and its paradoxical relation to diabetes mellitus
M Itagappa, SB Rao
Indian Journal of Clinical Biochemistry 2004
The Efficient Prevascularization Induced by Fibroblast Growth Factor 2 With a Collagen-Coated Device Improves the Cell Survival of a Bioartificial Pancreas
T Sakurai, A Satake, S Sumi, K Inoue, N Nagata, Y Tabata, J Miyakoshi
Pancreas 2004
Protective effects of a free radical scavenger, MCI-186, on high-glucose-induced dysfunction of human dermal microvascular endothelial cells
D Jiaojun, Y Takami, H Tanaka, R Yamaguchi, G Jingping, Q Chun, L ShuLiang, S Shimazaki, K Ogo
Wound Repair and Regeneration 2004
In Vitro Nonenzymatic Glycation Enhances the Role of Myoglobin as a Source of Oxidative Stress
A Roy, S Sen, AS Chakraborti
Free Radical Research 2004
Diabetes in der Praxis
WK Waldhäusl, FA Gries, WA Scherbaum
2004
International Textbook of Diabetes Mellitus
RA DeFronzo, L Mandarino, E Ferrannini
International Textbook of Diabetes Mellitus 2003
The role of advanced glycation in the pathogenesis of diabetic retinopathy
AW Stitt
Experimental and Molecular Pathology 2003
Changes in rat liver mitochondria with aging
H Bakala, E Delaval, M Hamelin, J Bismuth, C Borot-Laloi, B Corman, B Friguet
European Journal of Biochemistry 2003
Different influences of hyperglycemic duration on phosphorylated extracellular signal-regulated kinase 1/2 in rat heart
Z Naito, E Takashi, G Xu, T Ishiwata, K Teduka, M Yokoyama, N Yamada, Y Sugisaki, G Asano
Experimental and Molecular Pathology 2003
Diabetes mellitus and endothelial dysfunction: a central role for oxidative stress:
E Schulz, JF Keaney
Current Opinion in Endocrinology & Diabetes 2003
Blockade of receptor for advanced glycation endproducts: a new target for therapeutic intervention in diabetic complications and inflammatory disorders
Barry I Hudson, Loredana G Bucciarelli, Thoralf Wendt, Taichi Sakaguchi, Evanthia Lalla, Wu Qu, Yan Lu, Larisse Lee, David M Stern, Yoshifumi Naka, Ravichandran Ramasamy, Shi Du Yan, Shi Fang Yan, Vivette D'Agati, Ann Marie Schmidt
Archives of Biochemistry and Biophysics 2003
Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
GE Mann, DL Yudilevich, L Sobrevia
Physiological reviews 2003
The Amino-terminal Domains of the Ezrin, Radixin, and Moesin (ERM) Proteins Bind Advanced Glycation End Products, an Interaction That May Play a Role in the Development of Diabetic Complications
EA McRobert, M Gallicchio, G Jerums, ME Cooper, LA Bach
The Journal of biological chemistry 2003
Glucose, Glycation, and RAGE: Implications for Amplification of Cellular Dysfunction in Diabetic Nephropath y
T Wendt, N Tanji, J Guo, BI Hudson, A Bierhaus, R Ramasamy, B Arnold, PP Nawroth, SF Yan, V DAgati, AM Schmidt
Journal of the American Society of Nephrology : JASN 2003
Aminosalicylic acid reduces the antiproliferative effect of hyperglycaemia, advanced glycation endproducts and glycated basic fibroblast growth factor in cultured bovine aortic endothelial cells: comparison with aminoguanidine
Yasotha Duraisamy, John Gaffney, Mark Slevin, Christopher A Smith, Kenneth Williamson, Nessar Ahmed
Molecular and Cellular Biochemistry 2003
Role of nitric oxide, tetrahydrobiopterin and peroxynitrite in glucose toxicity-associated contractile dysfunction in ventricular myocytes
LB Esberg, J Ren
Diabetologia 2003
Receptor for advanced glycation endproducts (RAGE) and vascular inflammation: Insights into the pathogenesis of macrovascular complications in diabetes
T Wendt, L Bucciarelli, W Qu, Y Lu, SF Yan, DM Stern, AM Schmidt
Current Atherosclerosis Reports 2002
Advanced glycation end products and diabetic complications
AW Stitt, AJ Jenkins, ME Cooper
Expert Opinion on Investigational Drugs 2002
Oxidative stress in mice is dependent on the free glucose content of the diet
V Folmer, JC Soares, JB Rocha
The International Journal of Biochemistry & Cell Biology 2002
Nonenzymatic glycation of histones in vitro and in vivo
H Talasz, S Wasserer, B Puschendorf
Journal of Cellular Biochemistry 2002
Surface antigens of human mesangial cells: impact of growth surface or IL-1alpha
G Sengoelge, A Perschl, I Ferrara, WH Horl, G Sunder-Plassmann
Tissue Antigens 2002
Sugar-Induced Modification of Fibroblast Growth Factor 2 Reduces Its Angiogenic Activity in Vivo
F Facchiano, A Lentini, V Fogliano, S Mancarella, C Rossi, A Facchiano, MC Capogrossi
The American Journal of Pathology 2002
CML: a brief history
SR Thorpe, JW Baynes
International Congress Series 2002
Diabetes and advanced glycation endproducts
H Vlassara, MR Palace
Journal of Internal Medicine 2002
Formulation, Characterization, and Stability of Protein Drugs: Case Histories
R Pearlman, YJ Wang
2002
Advanced Glycation End Products Activate Endothelium Through Signal-Transduction Receptor RAGE: A Mechanism for Amplification of Inflammatory Responses
G Basta, G Lazzerini, M Massaro, T Simoncini, P Tanganelli, C Fu, T Kislinger, DM Stern, AM Schmidt, RD Caterina
Circulation 2002
A hydroxyl radical-like species oxidizes artery wall proteins in hyperglycemic cynomolgus monkeys: evidence for localized damage in early diabetic vascular disease
Subramaniam Pennathur, Janice D. Wagner, Christiaan Leeuwenburgh, Kenneth N. Litwak, Jay W. Heinecke
Journal of Clinical Investigation 2001
Biochemistry and molecular cell biology of diabetic complications
M Brownlee
Nature 2001
Diabetes and mass spectrometry
A Lapolla, D Fedele, P Traldi
Diabetes/Metabolism Research and Reviews 2001
The oxidative mechanism of heparin interferes with radical production by glucose and reduces the degree of glycooxidative modifications on human serum albumin
P Finotti, A Pagetta, T Ashton
European Journal of Biochemistry 2001
Advanced glycation: an important pathological event in diabetic and age related ocular disease
AW Stitt
The British journal of ophthalmology 2001
The role of glycation in the pathogenesis of diabetic polyneuropathy
RH King
Molecular pathology : MP 2001
Hox D3 Expression in Normal and Impaired Wound Healing
LA Uyeno, JA Newman-Keagle, I Cheung, TK Hunt, DM Young, N Boudreau
Journal of Surgical Research 2001
Heterogeneity Affecting Outcome From Acute Stroke Therapy: Making Reperfusion Worse
TA Kent, VM Soukup, RH Fabian
Stroke; a journal of cerebral circulation 2001
Diabetes and Cardiovascular Disease
A Angel, N Dhalla, G Pierce, P Singal
2001
Vaskuläre Veränderungen bei der diabetischen Retinopathie: Die zellulären und humoralen Interaktionsmechanismen in Antwort auf die Stoffwechselveränderungen
AM Joussen, AP Adamis, B Kirchhof
Spektrum der Augenheilkunde 2000
Negative consequences of glycation
M Brownlee
Metabolism 2000
A useful model to study the effect of high sugar concentrations upon growth and enzymic activities of toad embryos and larvae
F Francini, M Picasso, OR Rebolledo, A Salibián, JJ Gagliardino
Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 2000
Influence of diabetes on cardiac nitric oxide synthase expression and activity
K Stockklauser-Färber, T Ballhausen, A Laufer, P Rösen
Biochimica et Biophysica Acta 2000
Nichtenzymatische Glykierung und oxidativer Streß bei chronischen Erkrankungen und Diabetes mellitus
PP Nawroth, A Bierhaus, GE Vogel, MA Hofmann, M Zumbach, P Wahl, R Ziegler
Medizinische Klinik 1999
Emerging therapeutic targets in diabetic vascular disease
AM Schmidt, DM Stern
Expert Opinion on Therapeutic Targets 1999
Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation
NN Nissen, R Shankar, RL Gamelli, A Singh, LA DiPietro
Biochemical Journal 1999
Glucose-induced inhibition of angiogenesis in the rat sponge granuloma is prevented by aminoguanidine
AS Teixeira, SP Andrade
Life Sciences 1999
Aminoguanidine prevents impaired healing and deficient angiogenesis in diabetic rats
AS Teixeira, MV Caliari, OA Rocha, RD Machado, SP Andrade
Inflammation 1999
Carbamylated proteins activate glomerular mesangial cells and stimulate collagen deposition
M Shaykh, AA Pegoraro, W Mo, JA Arruda, G Dunea, AK Singh
Journal of Laboratory and Clinical Medicine 1999
Mechanical properties of mesenteric arteries in diabetic rats: consequences of outward remodeling
FR Crijns, BH Wolffenbuttel, JG de Mey, HA Boudier
American journal of physiology. Heart and circulatory physiology 1999
Activation of Receptor for Advanced Glycation End Products: A Mechanism for Chronic Vascular Dysfunction in Diabetic Vasculopathy and Atherosclerosis
AM Schmidt, SD Yan, JL Wautier, D Stern
Circulation research 1999
Overexpression of glyoxalase-I in bovine endothelial cells inhibits intracellular advanced glycation endproduct formation and prevents hyperglycemia-induced increases in macromolecular endocytosis
M Shinohara, PJ Thornalley, I Giardino, P Beisswenger, SR Thorpe, J Onorato, M Brownlee
Journal of Clinical Investigation 1998
Immunocytochemical detection of advanced glycated end products in rat renal tissue as a function of age and diabetes
M Bendayan
Kidney International 1998
Synovial inflammatory cells captured131I-β2-microglobulin in patients with dialysis related amyloidosis
JJ Kazama, M Arakawa, F Gejyo
Amyloid 1998
Vitamin E: Focus on Microcirculation
HA Lehr, P Vajkoczy, MD Menger
Microcirculation 1998
Growth Factors and the Kidney in Diabetes Mellitus
G Gambaro, B Baggio
Critical Reviews in Clinical Laboratory Sciences 1998
Immunological Evidence for Methylglyoxal-derived Modifications in Vivo: DETERMINATION OF ANTIGENIC EPITOPES
FA Shamsi, A Partal, C Sady, MA Glomb, RH Nagaraj
The Journal of biological chemistry 1998
Heparin-Induced Structural Modifications and Oxidative Cleavage of Human Serum Albumin in the Absence and Presence of Glucose. Implications for Transcapillary Leakage of Albumin in Hyperglycaemia
P Finotti, A Pagetta
European Journal of Biochemistry 1997
Advanced glycation end products in serum predict changes in the kidney morphology of patients with insulin-dependent diabetes mellitus
TJ Berg, HJ Bangstad, PA Torjesen, R Østerby, R Bucala, KF Hanssen
Metabolism 1997
Advanced glycation end products in human penis: elevation in diabetic tissue, site of deposition, and possible effect through inos or enos
AD Seftel, ND Vaziri, Z Ni, K Razmjouei, J Fogarty, N Hampel, J Polak, RZ Wang, K Ferguson, C Block, C Haas
Urology 1997
Early and advanced glycosylation end products. Kinetics of formation and clearance in peritoneal dialysis
MA Friedlander, YC Wu, A Elgawish, VM Monnier
Journal of Clinical Investigation 1996
BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells
I Giardino, D Edelstein, M Brownlee
Journal of Clinical Investigation 1996
?2-Microglobulin amyloidosis
À ARGILÉS
Nephrology 1996
THE CELLULAR AND MOLECULAR MECHANISMS OF DIABETIC COMPLICATIONS
GL King, M Brownlee
Endocrinology & Metabolism Clinics of North America 1996
Protein glycation in the kidney: Role in diabetes and aging
H Vlassara
Kidney International 1996
Regulation by metformin of the hexose transport system in vascular endothelial and smooth muscle cells
S Sasson, N Gorowits, HG Joost, GL King, E Cerasi, N Kaiser
British Journal of Pharmacology 1996
The Advanced Glycation End Product, N -(Carboxymethyl)lysine, Is a Product of both Lipid Peroxidation and Glycoxidation Reactions
MX Fu, JR Requena, AJ Jenkins, TJ Lyons, JW Baynes, SR Thorpe
The Journal of biological chemistry 1996
Regulatory role of eicosanoids in extracellular matrix overproduction induced by long-term exposure to high glucose in cultured rat mesangial cells
F Pricci, G Pugliese, P Menè, G Romeo, G Romano, G Galli, A Casini, CM Rotella, UD Mario, F Pugliese
Diabetologia 1996
Glucose modulates growth of gingival fibroblasts and periodontal ligament cells: correlation with expression of basic fibroblast growth factor
S Ohgi, PW Johnson
Journal of Periodontal Research 1996
Role of the Maillard Reaction in Diabetes Mellitus and Diseases of Aging:
SR Thorpe, JW Baynes
Drugs & Aging 1996
Decreased cell-mediated immunity in patients with non-insulin-dependent diabetes mellitus
F Chang
Diabetes Research and Clinical Practice 1995
ADVANCED PROTEIN GLYCOSYLATION IN DIABETES AND AGING
MB M.D
Annual Review of Medicine 1995
The role of growth factors in the development of diabetic retinopathy
PS Sharp
Metabolism 1995
Protein modification by the degradation products of ascorbate: formation of a novel pyrrole from the Maillard reaction of l-threose with proteins
RH Nagaraj, VM Monnier
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1995
Early AGEing and Alzheimer's
MA Smith, LM re, MP Vitek, VM Monnier, G Perry
Nature 1995
Increased endocytosis in retinal vascular endothelial cells grown in high glucose medium is modulated by inhibitors of nonenzymatic glycosylation
AW Stitt, U Chakravarthy, DB Archer, TA Gardiner
Diabetologia 1995
AGEing growth factors: a role in diabetic vascular disease?
JW Baynes
Journal of Clinical Investigation 1994

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