In this study, hepatic production of bile acid was considered together with intestinal cholesterol absorption as potential regulatory sites responsive to dietary cholesterol. Sequential liver biopsies were taken from 45 feral African green monkeys studied during three different diet periods. Low-fat Monkey Chow was fed during the baseline period, a cholesterol and fat-enriched diet was then fed for 12 wk during period 2, and finally, after a washout period of 10 wk, three subgroups were fed low-, moderate-, and high-cholesterol diets for 12 mo during period 3. The percentage of cholesterol absorbed in the intestine was significantly lower when higher levels of cholesterol were fed; however, this percentage was significantly and positively correlated to plasma cholesterol concentration at each dietary cholesterol level. Hepatic free and esterified cholesterol content were significantly elevated by dietary cholesterol challenge and remained elevated even after 20 wk of low-cholesterol diets. Hepatic mRNA abundance for cholesterol 7 alpha-hydroxylase (C7H) was significantly lower (approximately 60%) when the high-cholesterol diet was fed, with the decrease being greater than that seen for low density lipoprotein (LDL) receptor mRNA. At the same time, hepatic mRNA abundance for apolipoprotein B and hepatic lipase were not diet sensitive. C7H activity was decreased to a similar extent by diet as was C7H mRNA, although the correlation between enzyme activity and mRNA abundance was only r = 0.5, suggesting that dietary regulation includes factors in addition to transcriptional regulation. Activity and mRNA abundance of C7H remained decreased when liver esterified cholesterol content was reduced to only a two- to three-fold elevation over baseline, at a time when plasma cholesterol and hepatic LDL receptor mRNA abundance had returned to baseline levels. These data on liver C7H, obtained in one of the few primate species predisposed to cholesterol gallstone formation, support the hypothesis that the liver may attempt to downregulate intestinal cholesterol absorption by decreasing bile acid production when increased amounts of absorbed dietary cholesterol reach the liver. Presumably this represents attempted downregulation of intestinal cholesterol absorption by limiting bile acid availability as a means to maintain hepatic cholesterol balance.
L Rudel, C Deckelman, M Wilson, M Scobey, R Anderson
Title and authors | Publication | Year |
---|---|---|
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JM Lang, LR Sedgeman, L Cai, JD Layne, Z Wang, C Pan, R Lee, RE Temel, AJ Lusis |
Frontiers in Medicine | 2021 |
Mammalian Sterols: Novel Biological Roles of Cholesterol Synthesis Intermediates, Oxysterols and Bile Acids
D Rozman, R Gebhardt |
2020 | |
A case of gallstones in an African green monkey ( Chlorocebus aethiops )
D Kleinlützum, R Plesker |
Primate Biology | 2017 |
Food Ingredients That Inhibit Cholesterol Absorption
Elliot D Jesch, Timothy P Carr |
Preventive Nutrition and Food Science | 2017 |
The Clinical Chemistry of Laboratory Animals
DM Kurtz, GS Travlos |
The Clinical Chemistry of Laboratory Animals | 2017 |
Food Ingredients That Inhibit Cholesterol Absorption
Elliot D. Jesch, Timothy P. Carr |
Preventive Nutrition and Food Science | 2017 |
Rapid development of non-alcoholic steatohepatitis in Psammomys obesus (Israeli sand rat)
B Spolding, T Connor, C Wittmer, LL Abreu, A Kaspi, M Ziemann, G Kaur, A Cooper, S Morrison, S Lee, A Sinclair, Y Gibert, JL Trevaskis, JD Roth, A El-Osta, R Standish, K Walder |
PloS one | 2014 |
Rapid Development of Non-Alcoholic Steatohepatitis in Psammomys obesus (Israeli Sand Rat)
B Spolding, T Connor, C Wittmer, LL Abreu, A Kaspi, M Ziemann, G Kaur, A Cooper, S Morrison, S Lee, A Sinclair, Y Gibert, JL Trevaskis, JD Roth, A El-Osta, R Standish, K Walder, JM Lobaccaro |
PloS one | 2014 |
Advances in Food and Nutrition Research
N Siriwardhana, NS Kalupahana, N Moustaid-Moussa |
Marine Medicinal Foods - Implications and Applications - Animals and Microbes | 2012 |
The hypocholesterolemic activity of açaí (Euterpe oleracea Mart.) is mediated by the enhanced expression of the ATP-binding cassette, subfamily G transporters 5 and 8 and low-density lipoprotein receptor genes in the rat
MO de Souza, LS Silva, CL de Magalhães, BB de Figueiredo, DC Costa, ME Silva, ML Pedrosa |
Nutrition Research | 2012 |
Ursodeoxycholic acid lowers bile lithogenicity by regulating SCP2 expression in rabbit cholesterol gallstone models
Yunfeng Cui, Zhonglian Li, Erpeng Zhao, Ju Zhang, Naiqiang Cui |
EXCLI J | 2012 |
Effects of Adiposity on Plasma Lipid Response to Reductions in Dietary Saturated Fatty Acids and Cholesterol
MR Flock, MH Green, PM Kris-Etherton |
Advances in nutrition (Bethesda, Md.) | 2011 |
Effect of dietary glucosylceramide from sea cucumber on plasma and liver lipids in cholesterol-fed mice
Z Hossain, T Sugawara, K Aida, T Hirata |
Fisheries Science | 2011 |
Deciphering the nuclear bile acid receptor FXR paradigm
S Modica, RM Gadaleta, A Moschetta |
Nuclear receptor signaling | 2010 |
A chronic high-cholesterol diet paradoxically suppresses hepatic CYP7A1 expression in FVB/NJ mice
AS Henkel, KA Anderson, AM Dewey, MH Kavesh, RM Green |
Journal of lipid research | 2010 |
Thyroid hormone β receptor activation has additive cholesterol lowering activity in combination with atorvastatin in rabbits, dogs and monkeys
BR Ito, BH Zhang, EE Cable, X Song, JM Fujitaki, DA MacKenna, CE Wilker, B Chi, PD van Poelje, DL Linemeyer, MD Erion |
British Journal of Pharmacology | 2009 |
Estrogen decreases atherosclerosis in part by reducing hepatic acyl-CoA:cholesterol acyltransferase 2 (ACAT2) in monkeys
K Kavanagh, MA Davis, L Zhang, MD Wilson, TC Register, MR Adams, LL Rudel, JD Wagner |
Arteriosclerosis, thrombosis, and vascular biology | 2009 |
Sitosterol reduces messenger RNA and protein expression levels of Niemann-Pick C1-like 1 in FHs 74 Int cells
ED Jesch, JM Seo, TP Carr, JY Lee |
Nutrition Research | 2009 |
Inhibition of acyl-coenzyme A:cholesterol acyltransferase stimulates cholesterol efflux from macrophages and stimulates farnesoid X receptor in hepatocytes
S An, YS Jang, JS Park, BM Kwon, YK Paik, TS Jeong |
Experimental & molecular medicine | 2008 |
Liver Receptor Homolog-1 Regulates Bile Acid Homeostasis but Is Not Essential for Feedback Regulation of Bile Acid Synthesis
YK Lee, DR Schmidt, CL Cummins, M Choi, L Peng, Y Zhang, B Goodwin, RE Hammer, DJ Mangelsdorf, SA Kliewer |
Molecular Endocrinology | 2008 |
Loss of small heterodimer partner expression in the liver protects against dyslipidemia
HB Hartman, KD Lai, MJ Evans |
Journal of lipid research | 2008 |
Sourcebook of Models for Biomedical Research
PM Conn |
2008 | |
The pharmacological exploitation of cholesterol 7alpha-hydroxylase, the key enzyme in bile acid synthesis: from binding resins to chromatin remodelling to reduce plasma cholesterol
F Gilardi, N Mitro, C Godio, E Scotti, D Caruso, M Crestani, ED Fabiani |
Pharmacology & Therapeutics | 2007 |
CETP expression enhances liver HDL-cholesteryl ester uptake but does not alter VLDL and biliary lipid secretion
LM Harada, L Amigo, PM Cazita, AG Salerno, AA Rigotti, EC Quintão, HC Oliveira |
Atherosclerosis | 2007 |
Soybean Protein Hydrolysate Improves Plasma and Liver Lipid Profiles in Rats Fed High-Cholesterol Diet
SC Yang, SM Liu, HY Yang, YH Lin, JR Chen |
Journal of the American College of Nutrition | 2007 |
An overlapping binding site in the CYP7A1 promoter allows activation of FXR to override the stimulation by LXRα
Q Shang, L Pan, M Saumoy, JY Chiang, GS Tint, G Salen, G Xu |
AJP Gastrointestinal and Liver Physiology | 2007 |
Sitosterol reduces micellar cholesterol solubility in model bile
ED Jesch, TP Carr |
Nutrition Research | 2006 |
Molecular mechanisms of cholesterol absorption and transport in the intestine
DY Hui, PN Howles |
Seminars in Cell & Developmental Biology | 2005 |
Regulation of cholesterol 7α-hydroxylase mRNA expression in C57BL/6 mice fed an atherogenic diet
H Ando, S Tsuruoka, H Yamamoto, T Takamura, S Kaneko, A Fujimura |
Atherosclerosis | 2005 |
Glucagon and cAMP inhibit cholesterol 7α-hydroxylase (CYP7a1) gene expression in human hepatocytes: Discordant regulation of bile acid synthesis and gluconeogenesis
KH Song, JY Chiang |
Hepatology | 2005 |
Species-specific mechanisms for cholesterol 7α-hydroxylase (CYP7A1) regulation by drugs and bile acids
C Handschin, C Gnerre, DJ Fraser, C Martinez-Jimenez, R Jover, UA Meyer |
Archives of Biochemistry and Biophysics | 2005 |
Pediatric gallstone disease in familial hypobetalipoproteinemia
S Lancellotti, M Zaffanello, ED Leo, L Costa, A Lonardo, P Tarugi |
Journal of Hepatology | 2005 |
Approaches to measuring cholesterol absorption in humans
NR Matthan, AH Lichtenstein |
Atherosclerosis | 2004 |
Validation of a single-isotope-labeled cholesterol tracer approach for measuring human cholesterol absorption
Y Wang, CA Vanstone, WD Parsons, PJ Jones |
Lipids | 2004 |
Evaluation of 3-hydroxy-3-methylglutaryl-COA-reductase, cholesterol-7?-hydroxylase and acyl-COA:cholesterol acyltransferase activities: alternative chromatographic methods to separate metabolites
A Montoudis, S Boileau, L Simoneau, C Mounier, J Lafond |
Biomedical Chromatography | 2004 |
Branched-chain amino acid catabolism: unique segregation of pathway enzymes in organ systems and peripheral nerves
AJ Sweatt, M Wood, A Suryawan, R Wallin, MC Willingham, SM Hutson |
American journal of physiology. Endocrinology and metabolism | 2004 |
THEENZYMES, REGULATION,ANDGENETICSOFBILEACIDSYNTHESIS
DW Russell |
Annual Review of Biochemistry | 2003 |
Programming of initial steps in bile acid synthesis by breat-feeding vs. Formula-feeding in the baboon
GE Mott, EM Jackson, ML Klein, H Shan, J Pang, WK Wilson, CA McMahan |
Lipids | 2003 |
T HE E NZYMES , R EGULATION , AND G ENETICSOF B ILE A CID S YNTHESIS
DW Russell |
Annual Review of Biochemistry | 2003 |
Lipids and Atherosclerosis Annual 2003
I Schulman, R Heyman |
Lipids and Atherosclerosis Annual 2003 | 2003 |
Genetic background of cholesterol gallstone disease
A Kosters, M Jirsa, AK Groen |
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2003 |
Targeted Deletion of the Ileal Bile Acid Transporter Eliminates Enterohepatic Cycling of Bile Acids in Mice
PA Dawson, J Haywood, AL Craddock, M Wilson, M Tietjen, K Kluckman, N Maeda, JS Parks |
The Journal of biological chemistry | 2003 |
Cholesterol in Health and Disease
Ira Tabas |
Journal of Clinical Investigation | 2002 |
LXRα is the dominant regulator of CYP7A1 transcription
S Gupta, WM Pandak, PB Hylemon |
Biochemical and Biophysical Research Communications | 2002 |
Mice Expressing the Human CYP7A1 Gene in the Mouse CYP7A1 Knock-out Background Lack Induction of CYP7A1 Expression by Cholesterol Feeding and Have Increased Hypercholesterolemia When Fed a High Fat Diet
JY Chen, B Levy-Wilson, S Goodart, AD Cooper |
The Journal of biological chemistry | 2002 |
Regulation of cholesterol-7α-hydroxylase: BAREly missing a SHP
RA Davis, JH Miyake, TY Hui, NJ Spann |
Journal of lipid research | 2002 |
Separation of micelles and vesicles within lumenal aspirates from healthy humans: solubilization of cholesterol after a meal
L Yao, JE Heubi, DD Buckley, H Fierra, KD Setchell, NA Granholm, P Tso, DY Hui, LA Woollett |
Journal of lipid research | 2002 |
Regulation of cholesterol 7α-hydroxylase gene (CYP7A1) transcription by the liver orphan receptor (LXRα)
JY Chiang, R Kimmel, D Stroup |
Gene | 2001 |
Effects of CYP7A1 overexpression on cholesterol and bile acid homeostasis
WM Pandak, C Schwarz, PB Hylemon, D Mallonee, K Valerie, DM Heuman, RA Fisher, K Redford, ZR Vlahcevic |
AJP Gastrointestinal and Liver Physiology | 2001 |
THEROLE OFORPHANNUCLEARRECEPTORS IN THEREGULATION OFCHOLESTEROLHOMEOSTASIS
JJ Repa, DJ Mangelsdorf |
Annual Review of Cell and Developmental Biology | 2000 |
Correlation of Farnesoid X Receptor Coactivator Recruitment and Cholesterol 7α-Hydroxylase Gene Repression by Bile Acids
KS Bramlett, S Yao, TP Burris |
Molecular Genetics and Metabolism | 2000 |
Oxysterols: Modulators of Cholesterol Metabolism and Other Processes
GJ Schroepfer |
Physiological reviews | 2000 |
Hypocholesterolemic effect of anhydrous milk fat ghee is mediated by increasing the secretion of biliary lipids
MV Kumar, K Sambaiah, BR Lokesh |
The Journal of Nutritional Biochemistry | 2000 |
The Murine and Human Cholesterol 7α-Hydroxylase Gene Promoters Are Differentially Responsive to Regulation by Fatty Acids Mediated via Peroxisome Proliferator-activated Receptor α
SK Cheema, LB Agellon |
The Journal of biological chemistry | 2000 |
Consumption of Soy Protein Reduces Cholesterol Absorption Compared to Casein Protein Alone or Supplemented with an Isoflavone Extract or Conjugated Equine Estrogen in Ovariectomized Cynomolgus Monkeys
KA Greaves, MD Wilson, LL Rudel, JK Williams, JD Wagner |
The Journal of nutrition | 2000 |
Ileal bile acid transport regulates bile acid pool, synthesis, and plasma cholesterol levels differently in cholesterol-fed rats and rabbits
G Xu, BL Shneider, S Shefer, LB Nguyen, AK Batta, GS Tint, M Arrese, S Thevananther, L Ma, S Stengelin, W Kramer, D Greenblatt, M Pcolinsky, G Salen |
Journal of lipid research | 2000 |
Increasing dietary cholesterol induces different regulation of classic and alternative bile acid synthesis
G Xu, G Salen, S Shefer, GS Tint, LB Nguyen, TS Chen, D Greenblatt |
Journal of Clinical Investigation | 1999 |
Intestinal cholesterol absorption
PA Dawson, LL Rudel |
Current Opinion in Lipidology | 1999 |
Selective uptake of cholesteryl ester from low density lipoprotein is involved in HepG2 cell cholesterol homeostasis
MC Charest, D Rhainds, L Falstrault, T Matzouranis, L Brissette |
European Journal of Biochemistry | 1999 |
REGULATION OF BILE ACID BIOSYNTHESIS
ZR Vlahcevic, WM Pandak, RT Stravitz |
Gastroenterology Clinics of North America | 1999 |
Biliary cholesterol and bile acid excretion do not increase in hamsters fed cereal-based diets containing cholesterol
G Cai, TP Carr |
Metabolism | 1999 |
Comparative regulation of hepatic sterol 27-hydroxylase and cholesterol 7α-hydroxylase activities in the rat, guinea pig, and rabbit: Effects of cholesterol and bile acids
LB Nguyen, G Xu, S Shefer, GS Tint, A Batta, G Salen |
Metabolism | 1999 |
Complex feedback regulation of bile acid synthesis in the hamster: The role of newly synthesized cholesterol: Complex Feedback Regulation of Bile Acid Synthesis in the Hamster: The Role of Newly Synthesized Cholesterol
J Scheibner, M Fuchs, E Hörmann, EF Stange |
Hepatology | 1999 |
Dietary iron overload and induced lipid peroxidation are associated with impaired plasma lipid transport and hepatic sterol metabolism in rats
S Brunet, L Thibault, E Delvin, W Yotov, M Bendayan, E Levy |
Hepatology | 1999 |
Acyl-coenzyme A: Cholesterol acyltransferase inhibitor, avasimibe, stimulates bile acid synthesis and cholesterol 7?-hydroxylase in cultured rat hepatocytes andin vivo in the rat
SM Post, JP Zoeteweij, MH Bos, EC de Wit, R Havinga, F Kuipers, HM Princen |
Hepatology | 1999 |
Metabolism of Cholesterol Is Altered in the Liver of C3H Mice Fed Fats Enriched with Different C-18 Fatty Acids
SK Cheema, LB Agellon |
The Journal of nutrition | 1999 |
Lipoprotein and cholesterol metabolism in diet-induced coronary artery atherosclerosis in primates
LL Rudel, JS Parks, CC Hedrick, M Thomas, K Williford |
Progress in Lipid Research | 1998 |
Two 7?????hydroxylase enzymes in bile acid biosynthesis
M Schwarz, EG Lund, DW Russell |
Current Opinion in Lipidology | 1998 |
Sex steroids increase cholesterol 7α-hydroxylase mRNA in nonhuman primates
PL Colvin, JD Wagner, MR Adams, MG Sorci-Thomas |
Metabolism | 1998 |
Early diet influences hepatic hydroxymethyl glutaryl coenzyme A reductase and 7α-hydroxylase mRNA but not low-density lipoprotein receptor mRNA during development
AM Devlin, SM Innis, R Shukin, MF Rioux |
Metabolism | 1998 |
Reduced Hepatic Expression of CYP7A1 and CYP2C13 in Rats with Spontaneous Hyperlipidaemia
PJ Brassil, K Debri, H Nakura, S Kobayashi, DS Davies, RJ Edwards |
Biochemical Pharmacology | 1998 |
Identification of a Form of Acyl-CoA:Cholesterol Acyltransferase Specific to Liver and Intestine in Nonhuman Primates
RA Anderson, C Joyce, M Davis, JW Reagan, M Clark, GS Shelness, LL Rudel |
The Journal of biological chemistry | 1998 |
Regulation of classic and alternative bile acid synthesis in hypercholesterolemic rabbits: effects of cholesterol feeding and bile acid depletion
G Xu, G Salen, S Shefer, GS Tint, LB Nguyen, TT Parker, TS Chen, J Roberts, X Kong, D Greenblatt |
Journal of lipid research | 1998 |
U-shape relationship between change in dietary cholesterol absorptionand plasma lipoprotein responsiveness and evidence for extreme interindividualvariation in dietary cholesterol absorption in humans
E Sehayek, C Nath, T Heinemann, M McGee, CE Seidman, P Samuel, JL Breslow |
Journal of lipid research | 1998 |
Production rate determines plasma concentration of large high density lipoprotein in non-human primates
P Colvin, E Moriguchi, H Barrett, J Parks, L Rudel |
Journal of lipid research | 1998 |
The 3'-untranslated region of the mouse cholesterol 7alpha-hydroxylase mRNA contains elements responsive to post-transcriptional regulation by bile acids
LB Agellon, SK Cheema |
Biochemical Journal | 1997 |
Advances in Molecular and Cell Biology
KJ Clemetson |
Advances in Molecular and Cell Biology | 1997 |
Large Versus Small Unilamellar Vesicles Mediate Reverse Cholesterol Transport In Vivo Into Two Distinct Hepatic Metabolic Pools: Implications for the Treatment of Atherosclerosis
WV Rodrigueza, KD Mazany, AD Essenburg, ME Pape, TJ Rea, CL Bisgaier, KJ Williams |
Arteriosclerosis, thrombosis, and vascular biology | 1997 |
Dietary fats modulate the regulatory potential of dietary cholesterol on cholesterol 7 alpha-hydroxylase gene expression
SK Cheema, D Cikaluk, LB Agellon |
Journal of lipid research | 1997 |
Review of progress in sterol oxidations: 1987–1995
LL Smith |
Lipids | 1996 |
Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells
DP Wang, D Stroup, M Marrapodi, M Crestani, G Galli, JY Chiang |
Journal of lipid research | 1996 |
Unexpected inhibition of cholesterol 7 alpha-hydroxylase by cholesterol in New Zealand white and Watanabe heritable hyperlipidemic rabbits
G Xu, G Salen, S Shefer, GC Ness, LB Nguyen, TS Parker, TS Chen, Z Zhao, TM Donnelly, GS Tint |
Journal of Clinical Investigation | 1995 |
Treatment of the cholesterol biosynthetic defect in Smith-Lemli-Opitz syndrome reproduced in rats by BM 15.766
G Xu, G Salen, S Shefer, GC Ness, TS Chen, Z Zhao, L Salen, GS Tint |
Gastroenterology | 1995 |
Regulation of Hepatic 7α-Hydroxylase Expression and Response to Dietary Cholesterol in the Rat and Hamster
JD Horton, JA Cuthbert, DK Spady |
The Journal of biological chemistry | 1995 |
Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes
EA Kirk, GL Moe, MT Caldwell, JA Lernmark, DL Wilson, RC LeBoeuf |
Journal of lipid research | 1995 |
Psyllium reduces plasma LDL in guinea pigs by altering hepatic cholesterol homeostasis
ML Fernandez, LR Ruiz, AK Conde, DM Sun, SK Erickson, DJ McNamara |
Journal of lipid research | 1995 |
Pharmacological properties of a novel ACAT inhibitor (CP-113,818) in cholesterol-fed rats, hamsters, rabbits, and monkeys
CA Marzetta, YE Savoy, AM Freeman, CA Long, JL Pettini, RE Hagar, PB Inskeep, K Davis, AF Stucchi, RJ Nicolosi |
Journal of lipid research | 1994 |