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Citations to this article

Ligation of the T cell receptor complex results in activation of the Ras/Raf-1/MEK/MAPK cascade in human T lymphocytes.
R A Franklin, … , G L Johnson, E W Gelfand
R A Franklin, … , G L Johnson, E W Gelfand
Published May 1, 1994
Citation Information: J Clin Invest. 1994;93(5):2134-2140. https://doi.org/10.1172/JCI117209.
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Ligation of the T cell receptor complex results in activation of the Ras/Raf-1/MEK/MAPK cascade in human T lymphocytes.

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Abstract

Stimulation of T cells with antibodies directed towards the T cell receptor complex results in the activation of mitogen-associated protein kinase (MAPK). Two pathways have been described in other cell types that can lead to MAPK activation. One of these pathways involves the activation of Ras, leading to the activation of Raf-1, and the subsequent activation of MEK (MAPK or ERK kinase). The contribution of this pathway in T cells for anti-CD3 or phorbol myristate acetate (PMA)-mediated MAPK activation was examined. We detected the kinase activities of Raf-1 and MEK towards their substrates (MEK for Raf-1 and MAPK for MEK) in this pathway leading to the activation of MAPK. Stimulation of the T cells with either anti-CD3 antibody or PMA resulted in a rapid activation of both Ras and Raf-1. MEK activity towards kinase-active or -inactive recombinant MAPK also increased upon stimulation. In addition, both MAPK and p90rsk were activated in these cells. We suggest that activation of MAPK and the subsequent activation of ribosomal S6 kinase (p90rsk) occurs by the Ras/Raf-1/MEK cascade in T lymphocytes stimulated by ligation of the T cell receptor complex.

Authors

R A Franklin, A Tordai, H Patel, A M Gardner, G L Johnson, E W Gelfand

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Citations to this article in year 2015 (4)

Title and authors Publication Year
Lenalidomide enhances antitumor functions of chimeric antigen receptor modified T cells
P Otáhal, D Průková, V Král, M Fabry, P Vočková, L Latečková, M Trněný, P Klener
OncoImmunology 2015
The Potentiation of IFN-γ and Induction of Cytotoxic Proteins by Pegylated IL-10 in Human CD8 T Cells
IH Chan, V Wu, M Bilardello, E Mar, M Oft, PV Vlasselaer, JB Mumm
Journal of Interferon & Cytokine Research 2015
High-throughput workflow for identification of phosphorylated peptides by LC-MALDI-TOF/TOF-MS coupled to in situ enrichment on MALDI plates functionalized by ion landing: Workflow for identification of phosphopeptides
L Krásný, P Pompach, M Strnadová, R Hynek, K Vališ, V Havlíček, P Novák, M Volný
Journal of Mass Spectrometry 2015
Anti-adipogenic effect of erucin in early stage of adipogenesis by regulating Ras activity in 3T3-L1 preadipocytes
SY Chae, SG Seo, H Yang, JG Yu, SJ Suk, ES Jung, H Ji, JY Kwon, HJ Lee, KW Lee
Journal of Functional Foods 2015

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