Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Mechanism of the diastolic dysfunction induced by glycolytic inhibition. Does adenosine triphosphate derived from glycolysis play a favored role in cellular Ca2+ homeostasis in ferret myocardium?
H Kusuoka, E Marban
H Kusuoka, E Marban
Published March 1, 1994
Citation Information: J Clin Invest. 1994;93(3):1216-1223. https://doi.org/10.1172/JCI117075.
View: Text | PDF
Research Article

Mechanism of the diastolic dysfunction induced by glycolytic inhibition. Does adenosine triphosphate derived from glycolysis play a favored role in cellular Ca2+ homeostasis in ferret myocardium?

  • Text
  • PDF
Abstract

Several lines of evidence indicate that glycolysis is especially important for normal diastolic relaxation and for the maintenance of cellular ion homeostasis in myocardium. To elucidate whether the glycolytic flux of ATP contributes to diastolic tone and to the regulation of intracellular Ca2+, myocardial content of sugar phosphates ([SP]) and intracellular Ca2+ concentration ([Ca2+]i) were measured in isolated, perfused ferret hearts using nuclear magnetic resonance. Glucose and acetate were used as substrates for glycolysis and oxidative phosphorylation, respectively. Glycogen was effectively depleted after 15-min perfusion with glucagon (2 mg/liter), as verified by the lack of rise in [SP] during exposure to iodoacetate (100 microM) in substrate-free perfusate. Despite the fact that glycolytic flux had been blocked both by iodoacetate and by absence of substrate, end-diastolic left ventricular pressure (EDP) remained unchanged (P > 0.15, n = 6). The subsequent addition of glucose to the perfusate led to SP accumulation and a marked rise in EDP, with a significant correlation between EDP and [SP] (r = 0.86 +/- 0.04, P < 0.01, n = 6). A similar correlation was observed when glucose in the perfusate was replaced by 2-deoxyglucose (r = 0.78 +/- 0.09, P < 0.01, n = 3). Fluorine nuclear magnetic resonance measurements of [Ca2+]i verified that EDP faithfully reports changes in diastolic [Ca2+]i under the present experimental conditions. Thus, intracellular Ca2+ overload is caused by the accumulation of SP rather than by the inhibition of glycolysis per se. Glycolysis may appear to be important because its by-products are deleterious, and not necessarily because glycolytically derived ATP plays a favored role in ion homeostasis.

Authors

H Kusuoka, E Marban

×

Total citations by year

Year: 2024 2023 2022 2017 2014 2013 2011 2009 2008 2006 2005 2003 2002 2001 2000 1999 1998 1997 1996 1995 Total
Citations: 1 1 1 2 2 1 1 1 3 1 1 1 2 2 1 1 4 2 1 3 32
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (32)

Title and authors Publication Year
P53 Acetylation Exerts Critical Roles in Pressure Overload-Induced Coronary Microvascular Dysfunction and Heart Failure in Mice
He X, Cantrell AC, Williams QA, Gu W, Chen Y, Chen JX, Zeng H
Arteriosclerosis, thrombosis, and vascular biology 2024
P53 Acetylation Exerts Critical Roles In Pressure Overload Induced Coronary Microvascular Dysfunction and Heart Failure
He X, Cantrell AC, Williams QA, Gu W, Chen Y, Chen JX, Zeng H
2023
Knockout of TIGAR enhances myocardial phosphofructokinase activity and preserves diastolic function in heart failure.
He X, Zeng H, Cantrell AC, Williams QA, Chen JX
Journal of Cellular Physiology 2022
A comprehensive review of the bioenergetics of fatty acid and glucose metabolism in the healthy and failing heart in nondiabetic condition
A Gupta, B Houston
Heart Failure Reviews 2017
Cardiac Metabolic Deregulation Induced by the Tyrosine Kinase Receptor Inhibitor Sunitinib is rescued by Endothelin Receptor Antagonism
J Sourdon, F Lager, T Viel, D Balvay, R Moorhouse, E Bennana, G Renault, PL Tharaux, N Dhaun, B Tavitian
Theranostics 2017
Obesity preserves myocardial function during blockade of the glycolytic pathway
Dijon Henrique Salomé de Campos, André Soares Leopoldo, Ana Paula Lima-Leopoldo, André Ferreira do Nascimento, Silvio Assis de Oliveira-Junior, Danielle Cristina Tomaz da Silva, Mario Mateus Sugizaki, Carlos Roberto Padovani, Antonio Carlos Cicogna
Arquivos brasileiros de cardiologia 2014
Obesity Preserves Myocardial Function During Blockade of the Glycolytic Pathway
DH de Campos, AS Leopoldo, AP Lima-Leopoldo, AF Nascimento, SA de Oliveira-Junior, DC da Silva, MM Sugizaki, CR Padovani, AC Cicogna
Arquivos brasileiros de cardiologia 2014
Cardiac metabolism in heart failure: implications beyond ATP production
T Doenst, TD Nguyen, ED Abel
Circulation research 2013
Optimization of Cardiac Metabolism in Heart Failure
T Nagoshi, M Yoshimura, GM Rosano, GD Lopaschuk, S Mochizuki
Current pharmaceutical design 2011
Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2009
Angiotensin II downregulates the fatty acid oxidation pathway in adult rat cardiomyocytes via release of tumour necrosis factor- 
C Pellieux, C Montessuit, I Papageorgiou, R Lerch
Cardiovascular Research 2008
Growth hormone and heart failure: Oxidative stress and energetic metabolism in rats
FR Seiva, GM Ebaid, AV Castro, K Okoshi, A Nascimento, KK Rocha, CR Padovani, AC Cicogna, EL Novelli
Growth Hormone & IGF Research 2008
Ion Channels
B Fermini, BT Priest
Ion Channels 2008
Cytosolic energy reserves determine the effect of glycolytic sugar phosphates on sarcoplasmic reticulum Ca 2+ release in cat ventricular myocytes: Glycolytic sugar phosphates modulate Ca 2+ release
AV Zima, J Kockskämper, LA Blatter
The Journal of Physiology 2006
Myocardial Substrate Metabolism in the Normal and Failing Heart
WC Stanley, FA Recchia, GD Lopaschuk
Physiological reviews 2005
Myocardial Ischemia and Preconditioning
NS Dhalla, N Takeda, M Singh, A Lukas
2003
Cardiac dysfunction in mice lacking cytochrome- c oxidase subunit VIaH
NB Radford, B Wan, A Richman, LS Szczepaniak, JL Li, K Li, K Pfeiffer, H Schägger, DJ Garry, RW Moreadith
American journal of physiology. Heart and circulatory physiology 2002
Mechanical performance and glycolytic requirement in trout ventricular muscle
H Gesser
Journal of Experimental Zoology 2002
ATP from glycolysis is required for normal sodium homeostasis in resting fast-twitch rodent skeletal muscle
K Okamoto, W Wang, J Rounds, EA Chambers, DO Jacobs
American journal of physiology. Endocrinology and metabolism 2001
Postischemic Na + -K + -ATPase reactivation is delayed in the absence of glycolytic ATP in isolated rat hearts
JG van Emous, CL Vleggeert-Lankamp, MG Nederhoff, TJ Ruigrok, CJ van Echteld
American journal of physiology. Heart and circulatory physiology 2001
Changes in perfusion and fatty acid metabolism of rat heart with autoimmune myocarditis
E Tsujimura, H Kusuoka, K Fukughi, S Hasegawa, K Yufani, M Hori, S Hirono, T Izumi, T Nishimura
Annals of Nuclear Medicine 2000
Effects of Iodoacetate on Spontaneous Electrical Activity, Slow Wave, in the Circular Muscle of the Guinea-Pig Gastric Antrum
K Ogino, A Takai, Y Ishida, T Tomita
The Japanese Journal of Physiology 1999
Glycolytic pathway intermediates activate cardiac ryanodine receptors
H Kermode, WM Chan, AJ Williams, R Sitsapesan
FEBS Letters 1998
Metabolic inhibition in the perfused rat heart: evidence for glycolytic requirement for normal sodium homeostasis
J Dizon, D Burkhoff, J Tauskela, J Whang, P Cannon, J Katz
American journal of physiology. Heart and circulatory physiology 1998
Cardiac Metabolism in Health and Disease
S Abdel-Aleem, JE Lowe
1998
Glucose and glycogen utilisation in myocardial ischemia--changes in metabolism and consequences for the myocyte
L M King, L H Opie
Molecular and Cellular Biochemistry 1998
Calcium equally increases the internal calcium recirculation fraction before and after β-blockade in canine left ventricles
S Hosogi, J Araki, Y Syuu, S Suzuki, S Mohri, T Mikane, H Matsubara, T Ohe, M Hirakawa, H Suga
Heart and Vessels 1997
The Myocardium
H Zhu
The Myocardium 1997
Substrate effects on sarcolemmal permeability in the normoxic and hypoxic perfused rat heart
A Lochner, A Pentz, K Williams, E Tromp, I S Harper
Basic Research in Cardiology 1996
The role of Na+/K+ ATPase activity during low flow ischemia in preventing myocardial injury: A31P,23Na and87Rb NMR spectroscopic study
HR Cross, GK Radda, K Clarke
Magnetic Resonance in Medicine 1995
Functional Coupling Between Glycolysis and Sarcoplasmic Reticulum Ca 2+ Transport
KY Xu, JL Zweier, LC Becker
Circulation research 1995
Nuclear Magnetic Resonance Studies of Cationic and Energetic Alterations With Oxidant Stress in the Perfused Heart: Modulation With Pyruvate and Lactate
S Yanagida, CS Luo, M Doyle, GM Pohost, MM Pike
Circulation research 1995

← Previous 1 2 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts