The effect of insulinopenic diabetes on the expression of glucose transporters in the small intestine was investigated. Enterocytes were sequentially isolated from jejunum and ileum of normal fed rats, streptozotocin-diabetic rats, and diabetic rats treated with insulin. Facilitative glucose transporter (GLUT) 2, GLUT5, and sodium-dependent glucose transporter 1 protein content was increased from 1.5- to 6-fold in enterocytes isolated from diabetic animals in both jejunum and ileum. Insulin was able to reverse the increase in transporter protein expression seen after induction of diabetes. There was a four- to eightfold increase in the amount of enterocyte glucose transporter mRNA after diabetes with greater changes in sodium-dependent glucose transporter 1 and GLUT2 than in GLUT5 levels. In situ hybridization showed that after the induction of diabetes there was new hybridization in lower villus and crypt enterocytes that was reversed by insulin treatment. Thus, the increase in total hexose transport caused by diabetes is due to a premature expression of hexose transporters by enterocytes along the crypt-villus axis, causing a cumulative increase in enterocyte transporter protein during maturation. These changes are likely to represent an adaptive response by the organism to increase nutrient absorption in a perceived state of tissue starvation. These adaptive changes may lead to exacerbation of hyperglycemia in uncontrolled diabetes.
C F Burant, S Flink, A M DePaoli, J Chen, W S Lee, M A Hediger, J B Buse, E B Chang
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PS Leung |
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The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
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The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
2010 | |
The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
2010 | |
The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
2010 | |
The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
2010 | |
The Renin-Angiotensin System: Current Research Progress in The Pancreas
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2010 | |
The Renin-Angiotensin System: Current Research Progress in The Pancreas
PS Leung |
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HE Larsson, AJ Delli, SA Ivarsson, Å Lernmark |
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LA Drozdowski, MT Clandinin, AB Thomson |
World journal of gastroenterology : WJG | 2009 |
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AT Stearns, A Balakrishnan, A Tavakkolizadeh |
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TP Wong, ES Debnam, PS Leung |
American journal of physiology. Cell physiology | 2009 |
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GL Kellett, E Brot-Laroche, OJ Mace, A Leturque |
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V Douard, RP Ferraris |
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Dietary Gangliosides Enhance in Vitro Lipid Uptake in Weanling Rats
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L Drozdowski |
World journal of gastroenterology : WJG | 2006 |
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American journal of physiology. Endocrinology and metabolism | 2004 |
Age-associated changes in intestinal fructose uptake are not explained by alterations in the abundance of GLUT5 or GLUT2
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The Journal of Nutritional Biochemistry | 2004 |
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The age-associated decline in the intestinal uptake of glucose is not accompanied by changes in the mRNA or protein abundance of SGLT1
L Drozdowski, T Woudstra, G Wild, MT Clandindin, AB Thomson |
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Regulation of rat intestinal GLUT2 mRNA abundance by luminal and systemic factors
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American journal of physiology. Cell physiology | 1998 |
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DB Rhoads, DH Rosenbaum, H Unsal, KJ Isselbacher, LL Levitsky |
The Journal of biological chemistry | 1998 |
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Digestive Diseases and Sciences | 1998 |
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S Boyer, PA Sharp, ES Debnam, SA Baldwin, SK Srai |
FEBS Letters | 1996 |
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KL Madsen, D Ariano, RN Fedorak |
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KL Madsen, D Ariano, RN Fedorak |
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JF Desjeux |
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MA Hediger, Y Kanai, G You, S Nussberger |
The Journal of Physiology | 1995 |
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ES Debnam, MW Smith, PA Sharp, SK Srai, A Turvey, SJ Keable |
Pflügers Archiv - European Journal of Physiology | 1995 |
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SA Zoubi, TM Mayhew, RA Sparrow |
Virchows Archiv | 1995 |
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American journal of physiology. Cell physiology | 1994 |
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WS Lee, Y Kanai, RG Wells, MA Hediger |
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