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Citations to this article

Lipoxin A4 modulates transmigration of human neutrophils across intestinal epithelial monolayers.
S P Colgan, … , C Delp-Archer, J L Madara
S P Colgan, … , C Delp-Archer, J L Madara
Published July 1, 1993
Citation Information: J Clin Invest. 1993;92(1):75-82. https://doi.org/10.1172/JCI116601.
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Research Article Article has an altmetric score of 9

Lipoxin A4 modulates transmigration of human neutrophils across intestinal epithelial monolayers.

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Abstract

Neutrophil (PMN) migration across intestinal epithelial barriers, such as occurs in many disease states, results in modifications in epithelial barrier. Here, we investigated the impact of lipoxin A4 (LXA4), an eicosanoid with counterregulatory inflammatory roles, on PMN migration across cultured monolayers of the human intestinal epithelial cell line T84. Transepithelial migration of PMN was assessed in the apical-to-basolateral direction and in the basolateral-to-apical direction. In the apical-to-basolateral direction, preexposure of PMN to LXA4 (10 nM, 15 min) stimulated an 87 +/- 5% increase in transepithelial migration of PMN as determined by a PMN myeloperoxidase assay. The LXA4-elicited effect on transmigration was present throughout the 2-h assay period and was not secondary to LXA4 effects on epithelial monolayer integrity as judged by measurement of transepithelial resistance. PMN migration in the basolateral-to-apical direction was modulated by LXA4 with a comparable time- and concentration-dependence to that in the apical-to-basolateral direction. However, qualitative differences in how LXA4 modulates transmigration in the two opposing directions were observed. In the basolateral-to-apical direction, preexposure of PMN to LXA4 (10 nM, 15 min) diminished PMN transepithelial migration by 33 +/- 4%. Structure-function studies revealed that LXA4 and 11-trans-LXA4 (50% of LXA4 effect), but not LXB4, inhibited basolateral-to-apical PMN transmigration. The action of LXA4 was not sensitive to inhibitors of cyclooxygenase or specific leukotriene biosynthesis, but was sensitive to staurosporine, a protein kinase C inhibitor. These results suggest that migration of PMN across epithelia in the physiological direction may be qualitatively different following PMN exposure to eicosanoids. We propose that such retention of PMN at this specific anatomic location may serve an important role in mucosal defense.

Authors

S P Colgan, C N Serhan, C A Parkos, C Delp-Archer, J L Madara

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Citations to this article in year 2013 (6)

Title and authors Publication Year
Distinct signaling cascades elicited by different formyl Peptide receptor 2 (FPR2) agonists
F Cattaneo, M Parisi, R Ammendola
International journal of molecular sciences 2013
Lipoxin A 4 modulates adaptive immunity by decreasing memory B-cell responses via an ALX/FPR2-dependent mechanism: Cellular Immune Response
S Ramon, S Bancos, CN Serhan, RP Phipps
European Journal of Immunology 2013
Leukocyte-Epithelial Interactions and Mucosal Homeostasis
JD Matthews, CM Weight, CA Parkos
Toxicologic pathology 2013
The role of Lipoxin A4 in Cystic Fibrosis Lung Disease
V Urbach, G Higgins, P Buchanan, F Ringholz
Computational and Structural Biotechnology Journal 2013
Treating neutrophilic inflammation in COPD by targeting ALX/FPR2 resolution pathways
S Bozinovski, D Anthony, GP Anderson, LB Irving, BD Levy, R Vlahos
Pharmacology & Therapeutics 2013
Lack of activity of 15-epi-lipoxin A4 on FPR2/ALX and CysLT1 receptors in interleukin-8-driven human neutrophil function
A Planagumà, T Domenech, I Jover, I Ramos, S Sentellas, R Malhotra, M Miralpeix
Clinical & Experimental Immunology 2013

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