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Citations to this article

Phenotypic and functional characterization of human lymphocytes activated by interleukin-2 to directly inhibit growth of Cryptococcus neoformans in vitro.
S M Levitz, M P Dupont
S M Levitz, M P Dupont
Published April 1, 1993
Citation Information: J Clin Invest. 1993;91(4):1490-1498. https://doi.org/10.1172/JCI116354.
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Research Article Article has an altmetric score of 6

Phenotypic and functional characterization of human lymphocytes activated by interleukin-2 to directly inhibit growth of Cryptococcus neoformans in vitro.

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Abstract

Recently we demonstrated that the nonadherent (to plastic) fraction of human PBMC could be activated by IL-2 to inhibit Cryptococcus neoformans growth. Here we characterize the antifungal effector cells. Depletion by panning of natural killer (NK) (CD16+, CD56+) cells from nylon wool-treated, IL-2-activated PBMC markedly decreased lytic activity against a tumor cell target (K562) but did not affect antifungal activity. Panning out T (CD3+, CD5+) cells enhanced activity against tumor cells but partially abrogated activity against C. neoformans. IL-2-activated T cells of 95% purity, obtained by panning out NK cells from PBMC forming rosettes with sheep erythrocytes, had excellent antifungal activity but suboptimal antitumor activity. The nonrosetted cells (which were virtually free of T cells and enriched for NK cells) had both antitumor and antifungal activity, even if cultured without IL-2. CD4+, CD8+, and CD56+ cells, purified by positive selection by panning, directly inhibited cryptococcal growth. Conjugate formation between fungi and both CD56+ and CD5+ effector cells was demonstrated by videomicroscopy and immunoperoxidase staining. Thus, IL-2-activated T cells and NK cells form conjugates with and directly inhibit the growth of C. neoformans. To our knowledge, these data are the first demonstration of human T cells directly inhibiting growth of a microbial target.

Authors

S M Levitz, M P Dupont

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Year: 2024 2022 2021 2020 2018 2017 2015 2014 2011 2010 2009 2008 2007 2006 2005 2004 2002 2001 1999 1998 1997 1996 1995 1994 1993 Total
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Citations to this article (66)

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Frontiers in Oncology 2021
T11TS immunotherapy potentiates the repressed calcineurin‐NFAT signalling pathway of T cells in Cryptococcus neoformans infected rats: a cue towards T‐cell activation for antifungal immunity
SM Faruk, I Hazra, S Mondal, A Datta, S Moitra, PK Das, R Mishra, S Chaudhuri
Journal of Applied Microbiology 2020
β1 Integrins Are Required To Mediate NK Cell Killing of Cryptococcus neoformans
RF Xiang, SS Li, H Ogbomo, D Stack, CH Mody
Journal of immunology (Baltimore, Md. : 1950) 2018
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