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Citations to this article

Alterations in the protein composition of maturing phagosomes.
A Pitt, … , P D Stahl, A L Schwartz
A Pitt, … , P D Stahl, A L Schwartz
Published November 1, 1992
Citation Information: J Clin Invest. 1992;90(5):1978-1983. https://doi.org/10.1172/JCI116077.
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Research Article

Alterations in the protein composition of maturing phagosomes.

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Abstract

We investigated the protein composition of J774-E clone macrophage phagosomes isolated at different stages of phagolysosome biogenesis. Phagosomes formed by internalizing antibody-coated Staphylococcus aureus for 3 min followed by chase for 0, 4, 9, or 15 min were isolated by density gradient centrifugation. Enrichment and purity of the phagosome preparations were quantitated by radiolabeled ligand recovery, enzyme markers, and electron microscopy. One-dimensional SDS-PAGE analyses of the isolated phagosomes revealed virtually identical protein compositions. However, Western blot analyses with antibodies directed against selected proteins of known itineraries along the endocytic pathway demonstrated distinct differences in phagosome protein compositions. Accumulating within the maturing phagosome were the 31-kD subunit of the vacuolar proton pump, cathepsin D,beta-glucuronidase, the cation dependent mannose 6-phosphate receptor, and LAMP-1. Decreasing within the maturing phagosome were the FcII receptor, the mannose receptor, and alpha-adaptin. These results indicate that although the macrophage phagosome's total protein composition changes little during phagolysosome formation, the maturing phagosome both receives and eliminates, possibly by protein recycling, specific membrane and sequestered proteins.

Authors

A Pitt, L S Mayorga, P D Stahl, A L Schwartz

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The Journal of biological chemistry 1996
Phagocytosed Live Listeria monocytogenes Influences Rab5-regulated in Vitro Phagosome-Endosome Fusion
C Alvarez-Dominguez, AM Barbieri, W Berón, A Wandinger-Ness, PD Stahl
The Journal of biological chemistry 1996
Phagocytosis
EJ Brown
BioEssays 1995
Bafilomycin A1 inhibits lysosomal, phagosomal, and plasma membrane H+-ATPase and induces lysosomal enzyme secretion in macrophages
H Tapper, R Sundler
Journal of Cellular Physiology 1995
Membrane trafficking along the phagocytic pathway
W Berón, C Alvarez-Dominguez, L Mayorga, PD Stahl
Trends in Cell Biology 1995
Gaining insight into a complex organelle, the phagosome, using two-dimensional gel electrophoresis
J Burkhardt, LA Huber, H Dieplinger, A Blocker, G Griffiths, M Desjardins
Electrophoresis 1995
Parasite subversion of the host cell endocytic network
JY Channon, LH Kasper
Parasitology Today 1995
Characterization of the Mycobacterium tuberculosis phagosome and evidence that phagosomal maturation is inhibited
DL Clemens, MA Horwitz
Journal of Experimental Medicine 1995
A role for MARCKS, the alpha isozyme of protein kinase C and myosin I in zymosan phagocytosis by macrophages
LH Allen, A Aderem
Journal of Experimental Medicine 1995
Biogenesis of phagolysosomes: the ‘kiss and run’ hypothesis
M Desjardins
Trends in Cell Biology 1995
Trafficking of Intracellular Membranes:
MC Lima, N Düzgüneş, D Hoekstra
1995
Wortmannin, an inhibitor of phospholipase D activation, selectively blocks major histocompatibility complex class II-restricted antigen presentation
E Carrasco-Marín, C Alvarez-Domínguez, F Leyva-Cobián
European Journal of Immunology 1994
Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus
M Desjardins, LA Huber, RG Parton, G Griffiths
The Journal of Cell Biology 1994
Calcium-dependent fusion among endosomes
LS Mayorga, W Berón, MN Sarrouf, MI Colombo, C Creutz, PD Stahl
The Journal of biological chemistry 1994
Molecular characterization of phagosomes
M Desjardins, JE Celis, G van Meer, H Dieplinger, A Jahraus, G Griffiths, LA Huber
The Journal of biological chemistry 1994
Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex
V Pizon, M Desjardins, C Bucci, RG Parton, M Zerial
Journal of cell science 1994
Fusion between large phagocytic vesicles: targeting of yeast and other particulates to phagolysosomes that shelter the bacterium Coxiella burnetii or the protozoan Leishmania amazonensis in Chinese hamster ovary cells
PS Veras, C de Chastellier, MF Moreau, V Villiers, M Thibon, D Mattei, M Rabinovitch
Journal of cell science 1994

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