Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients.
R L Saltzman, … , M R Quirk, M C Jordan
R L Saltzman, … , M R Quirk, M C Jordan
Published November 1, 1992
Citation Information: J Clin Invest. 1992;90(5):1832-1838. https://doi.org/10.1172/JCI116059.
View: Text | PDF
Research Article

High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients.

  • Text
  • PDF
Abstract

Although viremia is a hallmark of disseminated cytomegalovirus (CMV) infection, not all viremic patients have visceral organ CMV disease. We used blot hybridization with a cloned subgenomic probe to quantitate viral DNA in blood leukocytes of 60 viremic patients (25 with solid organ transplants, 20 with AIDS, and 15 marrow recipients) who had different clinical manifestations of CMV infection. The results are expressed as pg of viral DNA/10 micrograms of leukocyte DNA. Patients with AIDS or with solid organ transplants who had CMV visceral organ disease had the largest amounts of viral DNA in their granulocytes (median 632 and 237 pg, respectively). These amounts were significantly greater than those in similar viremic patients without CMV visceral disease (17 and 21 pg; P < 0.005 and 0.002, respectively). All patients in the study with > 150 pg of CMV DNA in their granulocytes had visceral CMV disease. The amounts of viral DNA in granulocytes of AIDS and organ transplant patients with CMV retinitis were low (median 22 pg). Marrow transplant patients were unique in that the amounts of CMV DNA in granulocytes were low whether CMV visceral organ disease was present (17 pg) or absent (14 pg). We conclude that high levels of circulating CMV DNA in viremic AIDS and solid organ transplant patients reflect viral involvement of visceral organs but not the retina. In marrow recipients, the severity of CMV disease, even when fatal, is not reflected quantitatively in peripheral blood leukocytes.

Authors

R L Saltzman, M R Quirk, M C Jordan

×

Total citations by year

Year: 2016 2013 2009 2008 2006 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 Total
Citations: 1 1 1 2 3 1 2 2 3 7 2 7 8 7 4 5 1 57
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (57)

Title and authors Publication Year
Diagnostic Microbiology of the Immunocompromised Host
RT Hayden, DM Wolk, KC Carroll, YW Tang
2016
Clinical Utility of Viral Load in Management of Cytomegalovirus Infection after Solid Organ Transplantation
RR Razonable, RT Hayden
Clinical microbiology reviews 2013
Successful Immunotherapy of HCMV Disease Using Virus-Specific T Cells Expanded from an Allogeneic Stem Cell Transplant Recipient
GR Hill, SK Tey, L Beagley, T Crough, JA Morton, AD Clouston, P Whiting, R Khanna
American Journal of Transplantation 2009
Reverse Transcriptase-Polymerase Chain Reaction to Evaluate Human Cytomegalovirus Lytic Gene Expression
M Bergallo, C Costa, ME Terlizzi, S Margio, F Sidoti, S Astegiano, F Sinesi, R Cavallo
Molecular Biotechnology 2008
Spotting the owl: surreptitious cytomegalovirus disease in a renal transplant recipient
JL Tarkan, SB Woo, M Pavlakis, SR Johnson, LR Chirieac, SN Chimienti, DA Mandelbrot
Clinical Transplantation 2008
Evaluation of the COBAS Amplicor HCMV Monitor for early detection and monitoring of human cytomegalovirus infection after allogeneic stem cell transplantation
C Lengerke, T Ljubicic, C Meisner, J Loeffler, C Sinzger, H Einsele, H Hebart
Bone Marrow Transplantation 2006
A prospective study comparing quantitative Cytomegalovirus (CMV) polymerase chain reaction in plasma and pp65 antigenemia assay in monitoring patients after allogeneic stem cell transplantation
G Gentile, A Picardi, A Capobianchi, A Spagnoli, L Cudillo, T Dentamaro, A Tendas, L Cupelli, M Ciotti, A Volpi, S Amadori, P Martino, P Fabritiis
BMC Infectious Diseases 2006
Detección de ADN de CMV en plasma mediante PCR en tiempo real utilizando SYBR-Green I como señal de amplificación
E Varela-Ledo, S Romero-Yuste, P Ordóñez-Barbosa, P Romero-Jung, E Prieto-Rodríguez, A Aguilera-Guirao, B Regueiro-García
Enfermedades Infecciosas y Microbiología Clínica 2006
Clinical applications of real-time PCR for diagnosis and treatment of human cytomegalovirus infection in children
FH Chen, KT Samson, H Chen, SN Pan, ZX He, Y Iikura, S Shioda
Pediatric Allergy and Immunology 2004
Quantitation of cytomegalovirus DNA in cerebrospinal fluid and serum specimens from AIDS patients using a novel highly sensitive nested competitive PCR and the cobas amplicor CMV monitor?
D Tarrag�, ML Mateos, A Avell�n, MD P�rez-V�zquez, A Tenorio
Journal of Medical Virology 2003
Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients
H Li, JS Dummer, WR Estes, S Meng, PF Wright, YW Tang
Journal of clinical microbiology 2003
Early detection of cytomegalovirus (CMV) infection in bone marrow transplant patients by reverse transcription-PCR for CMV spliced late gene UL21.5: a two site evaluation
YS Boriskin, K Fuller, RL Powles, IB Vipond, PS Rice, JC Booth, EO Caul, PD Butcher
Journal of Clinical Virology 2002
Evaluation of the NucliSens CMV pp67 assay for detection and monitoring of human cytomegalovirus infection after allogeneic stem cell transplantation
H Hebart, T Rudolph, J Loeffler, J Middeldorp, T Ljubicic, G Jahn, H Einsele
Bone Marrow Transplantation 2002
Investigation of CMV disease in immunocompromised patients
VC Emery
Journal of clinical pathology 2001
Direct quantification of human cytomegalovirus immediate-early and late mRNA levels in blood of lung transplant recipients by competitive nucleic acid sequence-based amplification
AE Greijer, EA Verschuuren, MC Harmsen, CA Dekkers, HM Adriaanse, TH The, JM Middeldorp
Journal of clinical microbiology 2001
Evaluation of the Murex CMV DNA Hybrid Capture assay (version 2.0) for early diagnosis of cytomegalovirus infection in recipients of an allogeneic stem cell transplant
H Hebart, P Wuchter, J Loeffler, B Gscheidle, K Hamprecht, C Sinzger, G Jahn, K Dietz, L Kanz, H Einsele
Bone Marrow Transplantation 2001
Diagnosis of cytomegalovirus retinitis after heart transplantation
V Conraads, M Ieven, RM Smets, A Vorlat, H Janssen, A Moulijn, C Vrints
Transplant International 2000
A prospective study of a quantitative PCR ELISA assay for the diagnosis of CMV pneumonia in lung and heart-transplant recipients
L Barber, JJ Egan, J Lomax, Y Haider, N Yonan, AA Woodcock, AJ Turner, AJ Fox
The Journal of Heart and Lung Transplantation 2000
Therapeutic developments in cytomegalovirus retinitis
VF Hoffman, DJ Skiest
Expert Opinion on Investigational Drugs 2000
New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients
IG Sia, R Patel
Clinical microbiology reviews 2000
Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation
G Boivin, R Bélanger, R Delage, C Béliveau, C Demers, N Goyette, J Roy
Journal of clinical microbiology 2000
Clinical relevance of direct quantification of pp65 antigenemia using flow cytometry in solid organ and stem cell transplant recipients
AS Poirier-Toulemonde, N Milpied, D Cantarovich, JF Morcet, S Billaudel, BM Imbert-Marcille
Journal of clinical microbiology 2000
Evaluation of the COBAS AMPLICOR CMV MONITOR test for detection of viral DNA in specimens taken from patients after liver transplantation
IG Sia, JA Wilson, MJ Espy, CV Paya, TF Smith
Journal of clinical microbiology 2000
Cytomegalovirus Retinitis in the Era of Highly Active Antiretroviral Therapy (HAART)
DJ Skiest
The American Journal of the Medical Sciences 1999
Quantitative cytomegalovirus DNA level in the blood and its relationship to cytomegalovirus retinitis in patients with acquired immune deficiency syndrome11Digene Hybrid Capture System assays were provided by Digene Corporation, Beltsville, Maryland. The authors have no proprietary interest in the development or marketing of Digene Hybrid Capture System or competing tests
A Tufail, AA Moe, MJ Miller, EA Wagar, DA Bruckner, GN Holland
Ophthalmology 1999
Plasma cytomegalovirus (CMV) DNA load predicts CMV disease and survival in AIDS patients
SA Spector, R Wong, K Hsia, M Pilcher, MJ Stempien
Journal of Clinical Investigation 1998
Multiple organ involvement during experimental cytomegalovirus infection is associated with disseminated vascular pathology
MC Persoons, FS Stals, MC van dam Mieras, CA Bruggeman
The Journal of Pathology 1998
Quantitation of cytomegalovirus: methodologic aspects and clinical applications
M Boeckh, G Boivin
Clinical microbiology reviews 1998
Utility of major leukocyte subpopulations for monitoring secondary cytomegalovirus infections in renal-allograft recipients by PCR
P Schäfer, W Tenschert, L Cremaschi, K Gutensohn, R Laufs
Journal of clinical microbiology 1998
Evaluation of the AMPLICOR cytomegalovirus test with specimens from human immunodeficiency virus-infected subjects
G Boivin, J Handfield, E Toma, G Murray, R Lalonde, VJ Tevere, R Sun, MG Bergeron
Journal of clinical microbiology 1998
Evaluation of Murex CMV DNA hybrid capture assay for detection and quantitation of cytomegalovirus infection in patients following allogeneic stem cell transplantation
H Hebart, D Gamer, J Loeffler, C Mueller, C Sinzger, G Jahn, P Bader, T Klingebiel, L Kanz, H Einsele
Journal of clinical microbiology 1998
Management of Cytomegalovirus Infection after Solid-Organ or Stem-Cell Transplantation: Current Guidelines and Future Prospects
H Hebart, L Kanz, G Jahn, H Einsele
Drugs 1998
High Cytomegalovirus Antigenemia Levels and Cytomegalovirus Syndrome in Patients With AIDS
LG san Miguel, JL Casado, A Ca~nizares, M Lobo, A Guerrero
Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 1997
Quantity of cytomegalovirus viruria is a major risk factor for cytomegalovirus disease after renal transplantation
AV Cope, P Sweny, C Sabin, L Rees, PD Griffiths, VC Emery
Journal of Medical Virology 1997
Early effects of ganciclovir therapy on the quantity of cytomegalovirus DNA in leukocytes of immunocompromised patients
G Boivin, MR Quirk, BA Kringstad, M Germain, MC Jordan
Antimicrobial agents and chemotherapy 1997
Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay
P Schäfer, W Tenschert, K Gutensohn, R Laufs
Journal of clinical microbiology 1997
Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System
G Boivin, J Handfield, G Murray, E Toma, R Lalonde, JG Lazar, MG Bergeron
Journal of clinical microbiology 1997
Successful quantification of cytomegalovirus DNA by competitive PCR and detection with capillary electrophoresis
A Poirier-Toulemonde
Molecular and Cellular Probes 1997
Apport de la quantification de la charge virale ADN dans le suivi des maladies à cytomégalovirus chez les receveurs de transplantations rénales
BM Imbert, D Cantarovich, M Coste-Burel, R Lapied, S Billaudel
Revue Française des Laboratoires 1997
USEFULNESS OF DNA VIRAL LOAD QUANTIFICATION FOR CYTOMEGALOVIRUS DISEASE MONITORING IN RENAL AND PANCREAS/RENAL TRANSPLANT RECIPIENTS1:
BM Imbert-Marcille, D Cantarovich, V Ferre-Aubineau, B Richet, JP Soulillou, S Billaudel
Transplantation 1997
The intracellular localization of human cytomegalovirus DNA in peripheral blood leukocytes during active infections by high-resolution fluorescence in situ hybridization
H Hackstein, H Kirchner, G Jahn, G Bein
Archives of Virology 1996
Fluorescence in situ hybridization with cosmid clones for the detection of human cytomegalovirus DNA in peripheral blood leukocytes
H Hackstein, G Jahn, H Kirchner, G Bein
Histochemistry and Cell Biology 1996
Diagnosis of human cytomegalovirus infections in the immunocompromised host
G Gerna, M Zavattoni, E Percivalle, D Zella, M Torsellini, MG Revello
Clinical and Diagnostic Virology 1996
Effect of foscarnet on quantities of cytomegalovirus and human immunodeficiency virus in blood of persons with AIDS
HH Balfour, CV Fletcher, A Erice, WK Henry, EP Acosta, SA Smith, MA Holm, G Boivin, DH Shepp, CS Crumpacker, CA Eaton, SS Martin-Munley
Antimicrobial agents and chemotherapy 1996
Cytomegalovirus Antigenemia in Acquired Immunodeficiency Syndrome Patients With Untreated Cytomegalovirus Retinitis
CS Pannuti, EG Kallás, C Muccioli, RK Roland, EC Ferreira, SM Bueno, CL Canto, LS Boas, RU BELFORT
American Journal of Ophthalmology 1996
Antiviral Chemotherapy 4
J Mills, PA Volberding, L Corey
1996
Interest of quantitative pp65 cytomegalovirus antigenemia assay for human immunodeficiency virus-positive patients
S Ranger-Rogez, JP Couty, T Nicot, F Denis, JP Rogez, A Riche, P Weinbreck
Journal of clinical microbiology 1996
Continuous detection of CMV DNA in plasma of patients with advanced HIV infection implies the poorest prognosis
F Wang, M Brytting, G Bratt, P Ljungman, B Wahren
Clinical and Diagnostic Virology 1995
Quantitation of human cytomegalovirus glycoprotein H gene in cells using competitive PCR and a rapid fluorescence-based detection system
G Boivin, CA Olson, MR Quirk, SM St-Cyr, MC Jordan
Journal of Virological Methods 1995
Actualités sur la pathologie du cytomégalovirus humain
P Wattré, A Dewilde, PE Lobert
La Revue de Médecine Interne 1995
Technical and Biological Components of Marrow Transplantation
CD Buckner, RA Clift
1995
Monitoring of renal allograft recipients by quantisation of human cytomegalovirus genomes in peripheral blood leukocytes
JE Kühn, T Wendland, P Schäfer, K Möhring, U Wieland, M Elgas, HJ Eggers
Journal of Medical Virology 1994
Biphasic viremia and viral gene expression in leukocytes during acute cytomegalovirus infection of mice
TM Collins, MR Quirk, MC Jordan
Journal of virology 1994
Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients
G Gerna, M Furione, F Baldanti, A Sarasini
Journal of clinical microbiology 1994
Quantification of cytomegalovirus DNA in blood specimens from bone marrow transplant recipients by the polymerase chain reaction
BK Rawal, JC Booth, S Fernando, PD Butcher, RL Powles
Journal of Virological Methods 1994
Cytomegalovirus myocarditis following liver transplantation
WA Stack, HE Mulcahy, L Fenelon, JE Hegarty
Postgraduate medical journal 1994
Circulating endothelial giant cells permissive for human cytomegalovirus (HCMV) are detected in disseminated HCMV infections with organ involvement
E Percivalle, MG Revello, L Vago, F Morini, G Gerna
Journal of Clinical Investigation 1993

← Previous 1 2 3 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts