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Citations to this article

Eosinophils express interleukin 5 and granulocyte macrophage-colony-stimulating factor mRNA at sites of allergic inflammation in asthmatics.
D H Broide, … , M M Paine, G S Firestein
D H Broide, … , M M Paine, G S Firestein
Published October 1, 1992
Citation Information: J Clin Invest. 1992;90(4):1414-1424. https://doi.org/10.1172/JCI116008.
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Research Article Article has an altmetric score of 9

Eosinophils express interleukin 5 and granulocyte macrophage-colony-stimulating factor mRNA at sites of allergic inflammation in asthmatics.

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Abstract

IL-5 and granulocyte macrophage-colony-stimulating factor (GM-CSF) are important regulators of eosinophil survival, proliferation, and effector function. To determine whether IL-5 and/or GM-CSF are generated by eosinophils at sites of allergic inflammation, we have used in situ hybridization with 35S-labeled RNA probes to study the expression of IL-5 and GM-CSF mRNA in bronchoalveolar lavage (BAL) eosinophils derived from asthmatics (n = 5) before and after endobronchial allergen challenge. Endobronchial allergen challenge induced a significant airway eosinophilia (pre-allergen challenge 0.6 +/- 0.5% eosinophilia vs post-allergen challenge 48.2 +/- 25.6% eosinophilia). Post-allergen challenge eosinophils expressed IL-5 and GM-CSF mRNA, but did not express IL-1 beta or IL-2 mRNA. To determine whether the IL-5 mRNA-positive cells coexpressed GM-CSF mRNA, double mRNA labeling experiments with a digoxigenin-11-UTP nonradioactive labeled IL-5 RNA probe and a GM-CSF 35S-labeled RNA probe were performed. These studies demonstrated that individual eosinophils expressed one of four cytokine mRNA profiles (IL-5+, GM-CSF+, 34 +/- 13%; IL-5+, GM-CSF-, 34 +/- 5%; IL-5-, GM-CSF+, 11 +/- 9%; IL-5-, GM-CSF-, 21 +/- 25%). The expression of IL-5 and GM-CSF by eosinophils at sites of allergic inflammation in asthmatics may provide an important autocrine pathway, maintaining the viability and effector function of the recruited eosinophils.

Authors

D H Broide, M M Paine, G S Firestein

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Total citations by year

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Citations: 2 3 2 1 1 2 4 2 4 5 3 6 5 5 4 3 10 6 10 18 15 18 23 17 25 16 19 8 237
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Citations to this article in year 2013 (2)

Title and authors Publication Year
Signalling by the ßc family of cytokines
TR Hercus, U Dhagat, WL Kan, SE Broughton, TL Nero, M Perugini, JJ Sandow, RJ D’Andrea, PG Ekert, T Hughes, MW Parker, AF Lopez
Cytokine & Growth Factor Reviews 2013
Eosinophils in Health and Disease
GJ Gleich
Eosinophils in Health and Disease 2013

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ISSN: 0021-9738 (print), 1558-8238 (online)

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Referenced in 6 patents
Referenced in 1 clinical guideline sources
17 readers on Mendeley
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