Given the pivotal role suggested for IFN-gamma in immune diseases of the vascular wall, we investigated the effects of IFN-gamma on nitric oxide (NO) and endothelin-1 (ET-1) expression in bovine aortic endothelial cells (BAEC). We have previously reported that TNF-alpha enhanced NO synthase activity in BAEC as assessed by quantifying release of bioactive NO with reporter monolayers and measuring conversion of L-[14C]arginine to L-[14C] citrulline. In murine macrophages IFN-gamma synergizes with TNF-alpha or lipopolysaccharide to induce robust increases in calcium-independent NO synthase activity. In this study we have found that IFN-gamma alone failed to have a significant effect on NO synthase activity in BAEC. In contrast to murine macrophages, IFN-gamma inhibited TNF-alpha-stimulated induction of endothelial NO synthase activity in a concentration-dependent manner. This observation suggests that there is major difference in the response of BAEC and murine macrophages to IFN-gamma. A second major aim of this study was to determine the effect of IFN-gamma on preproET-1 mRNA expression and ET-1 secretion rates in BAEC. IFN-gamma alone had little or no effect on ET-1 mRNA levels and basal ET release when measured for 8 h. However, cotreatment with IFN-gamma potentiated the stimulatory effect of TNF-alpha on BAEC ET-1 mRNA transcript levels and ET release. In contrast, pretreatment of cells with IFN-gamma for 16-24 h blunted the stimulatory effect of TNF-alpha. These findings suggest that endothelial cell expression of vasoactive mediators is modified by the temporal interplay of at least two immune mediators, IFN-gamma and TNF-alpha.
S Lamas, T Michel, T Collins, B M Brenner, P A Marsden
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Crosstalk mechanisms between glomerular endothelial cells and podocytes in renal diseases and kidney transplantation
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I Parra-Izquierdo, T Sánchez-Bayuela, J López, C Gómez, E Pérez-Riesgo, JA Román, MS Crespo, M Yacoub, AH Chester, C García-Rodríguez |
International journal of molecular sciences | 2021 |
Protein Interactions at Endothelial Junctions and Signaling Mechanisms Regulating Endothelial Permeability
YA Komarova, K Kruse, D Mehta, AB Malik |
Circulation research | 2017 |
The Role of Nitric Oxide in Metabolic Regulation of Dendritic Cell Immune Function
P Thwe, E Amiel |
Cancer Letters | 2017 |
Immune-Mediated Vascular Injury and Dysfunction in Transplant Arteriosclerosis
A Rossum, I Laher, JC Choy |
Frontiers in immunology | 2015 |
Reference Module in Biomedical Sciences
DL Sackett, RB Haynes |
2014 | |
Interferon-γ promotes vascular remodeling in human microvascular endothelial cells by upregulating endothelin (ET)-1 and transforming growth factor (TGF) β2
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Journal of Cellular Physiology | 2013 |
Green tea catechins decrease oxidative stress in surgical menopause-induced overactive bladder in a rat model: ANTIOXIDANT EFFECTS OF CATECHINS ON POST-MENOPAUSAL RAT BLADDER
YS Juan, SM Chuang, YL Lee, CY Long, TH Wu, WC Chang, RM Levin, KM Liu, CH Huang |
BJU International | 2012 |
The fate of renal allografts hinges on responses of the microvascular endothelium
VK Mannam, RE Lewis, JM Cruse |
Experimental and Molecular Pathology | 2012 |
The effect of interferon γ on endothelial cell nitric oxide production and apoptosis
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Advanced Biomedical Research | 2012 |
Differential Roles of Endothelin-1 in Angiotensin II-Induced Atherosclerosis and Aortic Aneurysms in Apolipoprotein E-Null Mice
RS Suen, SN Rampersad, DJ Stewart, DW Courtman |
The American Journal of Pathology | 2011 |
Capric Acid Inhibits NO Production and STAT3 Activation during LPS-Induced Osteoclastogenesis
EJ Park, SA Kim, YM Choi, HK Kwon, W Shim, G Lee, S Choi |
PloS one | 2011 |
dsRNA activation of endothelin-1 and markers of vascular activation in endothelial cells and fibroblasts
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Annals of the rheumatic diseases | 2010 |
Interferon-γ Is Increased in Patients with Primary Sjogren's Syndrome and Raynaud's Phenomenon
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Nitric oxide enhances osteoclastogenesis possibly by mediating cell fusion
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Nitric Oxide | 2009 |
Relative Reduction of Endothelial Nitric-Oxide Synthase Expression and Transcription in Atherosclerosis-Prone Regions of the Mouse Aorta and in an in Vitro Model of Disturbed Flow
D Won, SN Zhu, M Chen, AM Teichert, JE Fish, CC Matouk, M Bonert, M Ojha, PA Marsden, MI Cybulsky |
The American Journal of Pathology | 2007 |
Endothelins Mediate Neutrophil Activation, ProMMP-9 Release and Endothelial Cell Detachment
MC Toffoli, BH Gabra, CF Teixeira, P Sirois, S Jancar |
Inflammation | 2007 |
Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells
K Palatka, Z Serfozo, Z Veréb, R Bátori, B Lontay, Z Hargitay, Z Nemes, M Udvardy, F Erdodi, I Altorjay |
World journal of gastroenterology : WJG | 2006 |
Epigenetic Basis for the Transcriptional Hyporesponsiveness of the Human Inducible Nitric Oxide Synthase Gene in Vascular Endothelial Cells
GC Chan, JE Fish, IA Mawji, DD Leung, AC Rachlis, PA Marsden |
Journal of immunology (Baltimore, Md. : 1950) | 2005 |
Elevated Endothelin-1 Expression in Dogs with Heartworm Disease
T UCHIDE, K SAIDA |
Journal of Veterinary Medical Science | 2005 |
Cooperative Expression of Monocyte Chemoattractant Protein 1 Within the Bovine Corpus Luteum: Evidence of Immune Cell-Endothelial Cell Interactions in a Coculture System1
AR Liptak, BT Sullivan, LE Henkes, MP Wijayagunawardane, A Miyamoto, JS Davis, BR Rueda, DH Townson |
Biology of reproduction | 2005 |
Hydrogen peroxide regulation of bovine endothelin-converting enzyme-1
S López-Ongil, M Saura, C Zaragoza, L Gónzalez-Santiago, M Rodrı́guez-Puyol, CJ Lowenstein, D Rodrı́guez-Puyol |
Free radical biology & medicine | 2002 |
Temporal and Segmental Distribution of Constitutive and Inducible Nitric Oxide Synthases after Traumatic Spinal Cord Injury: Effect of Aminoguanidine Treatment
K Chatzipanteli, R Garcia, AE Marcillo, KE Loor, S Kraydieh, WD Dietrich |
Journal of Neurotrauma | 2002 |
Peroxisome proliferator-activated receptor agonists, hyperlipidaemia, and atherosclerosis
H Vosper, GA Khoudoli, TL Graham, CN Palmer |
Pharmacology & Therapeutics | 2002 |
Neurodegeneration and glia response in rat hippocampus following nitro-L-arginine methyl ester (L-NAME)
GJ Harry, R Sills, MJ Schlosser, WE Maier |
Neurotoxicity Research | 2001 |
Coordinate regulation of endothelin and adrenomedullin secretion by oxidative stress in endothelial cells
T Saito, H Itoh, TH Chun, Y Fukunaga, J Yamashita, K Doi, T Tanaka, M Inoue, K Masatsugu, N Sawada, S Sakaguchi, H Arai, M Mukoyama, K Tojo, T Hosoya, K Nakao |
American journal of physiology. Heart and circulatory physiology | 2001 |
Blockade of the p38 Mitogen-activated Protein Kinase Pathway Inhibits Inducible Nitric Oxide Synthase and Interleukin-6 Expression in MC3T3E-1 Osteoblasts
HJ Chae, SC Kim, SW Chae, N an, HH Kim, ZH Lee, HR Kim |
Pharmacological Research | 2001 |
Countervailing Influence of Tumor Necrosis Factor-α and Nitric Oxide in Endotoxemia
EA Jaimes, DD Castillo, MS Rutherford, L Raij |
Journal of the American Society of Nephrology : JASN | 2001 |
The role of nitric oxide in the responses of the ovine digital artery to vasoactive agents and modification of these responses by endotoxin and cytokines
P Pawson, J Reid, AM Nolan |
British Journal of Pharmacology | 2000 |
Endothelins in the urinary tract
ME Sullivan, FH Mumtaz, MA Khan, MR Dashwood, CS Thompson, DP Mikhailidis, RJ Morgan |
BJU International | 2000 |
Involvement of Rho GTPases in the transcriptional inhibition of preproendothelin-1 gene expression by simvastatin in vascular endothelial cells
O Hernández-Perera, D Pérez-Sala, E Soria, S Lamas |
Circulation research | 2000 |
Peroxisome proliferator-activated receptor activators inhibit thrombin-induced endothelin-1 production in human vascular endothelial cells by inhibiting the activator protein-1 signaling pathway
P Delerive, F Martin-Nizard, G Chinetti, F Trottein, JC Fruchart, J Najib, P Duriez, B Staels |
Circulation research | 1999 |
Nitric Oxide Fully Protects against UVA-induced Apoptosis in Tight Correlation with Bcl-2 Up-regulation
CV Suschek, V Krischel, D Bruch-Gerharz, D Berendji, J Krutmann, KD Kröncke, V Kolb-Bachofen |
The Journal of biological chemistry | 1999 |
Effects of the 3-hydroxy-3-methylglutaryl-CoA reductase inhibitors, atorvastatin and simvastatin, on the expression of endothelin-1 and endothelial nitric oxide synthase in vascular endothelial cells
O Hernández-Perera, D Pérez-Sala, J Navarro-Antolín, R Sánchez-Pascuala, G Hernández, C Díaz, S Lamas |
Journal of Clinical Investigation | 1998 |
Verotoxin and ricin have novel effects on preproendothelin-1 expression but fail to modify nitric oxide synthase (ecNOS) expression and NO production in vascular endothelium
MM Bitzan, Y Wang, J Lin, PA Marsden |
Journal of Clinical Investigation | 1998 |
CsA and FK506 up-regulate eNOS expression: Role of reactive oxygen species and AP-1
J Navarro-Antolin, O Hernandez-Perera, S Lopez-Ongil, M Rodriguez-Puyol, D Rodriguez-Puyol, S Lamas |
Kidney International | 1998 |
Role of reactive oxygen species in the signalling cascade of cyclosporine A-mediated up-regulation of eNOS in vascular endothelial cells
S López-Ongil, O Hernández-Perera, J Navarro-Antolín, GP de Lema, M Rodríguez-Puyol, S Lamas, D Rodríguez-Puyol |
British Journal of Pharmacology | 1998 |
Essential role for endothelin ETBreceptors in fever induced by LPS (E. coli) in rats
AS Fabricio, CA Silva, GA Rae, P D'Orléans-Juste, GE Souza |
British Journal of Pharmacology | 1998 |
Endothelin Receptors and Signaling Mechanisms
DM Pollock, RF Highsmith |
1998 | |
Effects of anti-endothelial cell antibody in pre-eclampsia on endothelin-1 release from cultured endothelial cells
T Yamamoto, Y Takahashi, S Kuno, Y Geshi, Y Sasamori, H Mori |
Immunology and Cell Biology | 1997 |
Endothelial Activation by Cytokines
A Mantovani, S Sozzani, M Introna |
Annals of the New York Academy of Sciences | 1997 |
Nitric Oxide and the Kidney
MS Goligorsky, SS Gross |
1997 | |
Endothelin in organ transplantation
B Watschinger, MH Sayegh |
American Journal of Kidney Diseases | 1996 |
Interleukin-13 inhibits inducible nitric oxide synthase expression in human mesangial cells
M Saura, R Martínez-Dalmau, A Minty, D Pérez-Sala, S Lamas |
Biochemical Journal | 1996 |
Consequences of alteration in capillary permeability
GE Plante, M Chakir, K Ettaouil, S Lehoux, P Sirois |
Canadian Journal of Physiology and Pharmacology | 1996 |
Nitric oxide and anti-cancer therapy
SM Sagar, G Singh, DI Hodson, AC Whitton |
Cancer Treatment Reviews | 1995 |
Nitric Oxide
RA Schroeder, PC Kuo |
Anesthesia & Analgesia | 1995 |
Regulation of inducible nitric oxide synthase expression in rat mesangial cells and isolated glomeruli
M Saura, S López, MR Puyol, DR Puyol, S Lamas |
Kidney International | 1995 |
Nitric oxide inhibits establishment of macroschizont-infected cell lines and is produced by macrophages of calves undergoing bovine tropical theileriosis or East Coast fever
AE Visser, A Abraham, LJ Sakyi, CG Brown, PM Preston |
Parasite Immunology | 1995 |
Upregulation and modulation of inducible nitric oxide synthase in rat cardiac allografts with chronic rejection and transplant arteriosclerosis
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Circulation | 1995 |
Signal sorting by G-protein-linked receptors
G Milligan |
Advances in pharmacology (San Diego, Calif.) | 1995 |
Differential Regulation of Adhesion Molecule Expression by Human Cerebrovascular and Umbilical Vein Endothelial Cells
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Endothelium : journal of endothelial cell research | 1995 |
Regulation of nitric oxide synthesis by proinflammatory cytokines in human umbilical vein endothelial cells. Elevations in tetrahydrobiopterin levels enhance endothelial nitric oxide synthase specific activity
P Rosenkranz-Weiss, WC Sessa, S Milstien, S Kaufman, CA Watson, JS Pober |
Journal of Clinical Investigation | 1994 |
Glomerular thrombosis in pregnancy: Role of the L-arginine-nitric oxide pathway
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Kidney International | 1994 |
Vascular Endothelium
MA Gimbrone, MI Cybulsky, N Kume, T Collins, N Resnick |
Annals of the New York Academy of Sciences | 1994 |
Regulation of Endothelin-1 Biosynthesisa
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Annals of the New York Academy of Sciences | 1994 |
Primary cultures of rat islet capillary endothelial cells. Constitutive and cytokine-inducible macrophagelike nitric oxide synthases are expressed and activities regulated by glucose concentration
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The American Journal of Pathology | 1994 |
SPONTANEOUS ENDOTHELIN PRODUCTION BY CIRCULATING MONONUCLEAR CELLS FROM PATIENTS WITH CHRONIC HEART FAILURE BUT NOT FROM NORMAL SUBJECTS
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Clinical and Experimental Pharmacology and Physiology | 1994 |
Reversal of bradykinin-induced relaxation to contraction after interferon-γ in bovine isolated mesenteric arteries
SJ de Kimpe, W Tielemans, DV Heuven-Nolsen, FP Nijkamp |
European Journal of Pharmacology | 1994 |
Nitric Oxide in the Kidney: Synthesis, Localization, and Function
S Bachmann, P Mundel |
American Journal of Kidney Diseases | 1994 |
Free Radical Chemistry of Nitric Oxide
B Freeman |
Chest | 1994 |
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R Mccarron, L Wang, D Stanimirovic, M Spatz |
Neuroscience Letters | 1993 |
Endothelins in the Kidney: Physiology and Pathophysiology
DE Kohan |
American Journal of Kidney Diseases | 1993 |
Nitric Oxide Synthase mRNA in Endothelial Cells: Synergistic Induction by Interferon-γ, Tumor Necrosis Factor-α and Lipopolysaccharide and Inhibition by Dexamethasone
T MaruMo, T Nakaki, H Adachi, H Esumi, H Suzuki, T Saruta, R Kato |
The Japanese Journal of Pharmacology | 1993 |