Recombinant interleukin-12 suppresses the synthesis of immunoglobulin E by interleukin-4 stimulated human lymphocytes.

M Kiniwa; M Gately; U Gubler; R Chizzonite; C Fargeas; G Delespesse

doi:10.1172/JCI115846

University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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University of Montreal, Notre-Dame Hospital Research Center, Quebec, Canada.

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Published in Volume 90, Issue 1 on July 1, 1992
J Clin Invest. 1992;90(1):262–266. https://doi.org/10.1172/JCI115846.
© 1992 The American Society for Clinical Investigation

Published July 1, 1992 - Version history

Interleukin-12 is a recently discovered lymphokine displaying an array of in vitro activities suggesting a major role in protective immunity against infectious agents like viruses. This study provides evidence that IL-12 may also be implicated in the selection of the immunoglobulin isotypes. We show that picomolar concentrations of rIL-12 markedly inhibit the synthesis of IgE by IL-4-stimulated PBMC. The suppression of IgE is observed at the protein and at the mRNA levels, it is isotype specific, and it is abolished by neutralizing anti-IL-12 mAbs. IL-12 may suppress IgE synthesis by: (a) inducing the production of IFN-gamma, a known inhibitor of IgE synthesis and (b) by a novel mechanism which is IFN-gamma independent. The best evidence for this is from studies on IgE synthesis by IL-4-plus hydrocortisone-stimulated umbilical cord blood lymphocytes, which do not produce detectable amounts of IFN-gamma. In such cultures, rIL-12 inhibits IgE synthesis even in the presence of a large excess of neutralizing anti-IFN-gamma mAb.

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