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Research Article Free access | 10.1172/JCI115737

Enhancement of incisional wound healing and neovascularization in normal rats by thrombin and synthetic thrombin receptor-activating peptides.

D H Carney, R Mann, W R Redin, S D Pernia, D Berry, J P Heggers, P G Hayward, M C Robson, J Christie, and C Annable

Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.

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Published May 1, 1992 - More info

Published in Volume 89, Issue 5 on May 1, 1992
J Clin Invest. 1992;89(5):1469–1477. https://doi.org/10.1172/JCI115737.
© 1992 The American Society for Clinical Investigation
Published May 1, 1992 - Version history
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Abstract

To better define thrombin-receptor interactions, we synthesized human thrombin peptides and identified binding-domain peptides that bind thrombin receptors and activate mitogenic signals (Glenn, K.C., G.H. Frost, J.S. Bergmann, and D.H. Carney. 1988. Pept. Res. 1:65-73). Treatment of full dermal dorsal incisions with a single topical application of thrombin receptor-activating peptide (TRAP-508) or human alpha-thrombin in saline enhances 7-d incisional breaking strength in normal rats up to 82% or 55% over saline-treated controls, respectively. Control wounds require approximately 11.5 d to achieve breaking strength equivalent to TRAP-treated wounds at day 7. Thus, a single application of TRAP accelerates healing, shifting the time course forward by up to 4.5 d. Histological comparisons at day 7 show more type I collagen, less evidence of prolonged inflammation, and an increase in number and maturity of capillaries in TRAP- and thrombin-treated incisions. Angiograms also show 50-65% more functional vascularization going across thrombin- and TRAP-treated surgical incisions. Thus, alpha-thrombin and thrombin peptides, such as those released following injury, appear to initiate or enhance signals required for neovascularization and wound healing. The ability to accelerate normal wound healing events with synthetic peptides representing receptor binding domains of human thrombin may offer new options for management of wound healing in man.

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