Advertisement
Research Article Free access | 10.1172/JCI115720
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Larsson, C. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Shepherd, J. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Nakamura, Y. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Blomberg, C. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Weber, G. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Werelius, B. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Hayward, N. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Teh, B. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Tokino, T. in: JCI | PubMed | Google Scholar
Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden.
Find articles by Seizinger, B. in: JCI | PubMed | Google Scholar
Published April 1, 1992 - More info
Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominantly inherited predisposition to neoplastic lesions of the parathyroids, pancreas, and the pituitary. We have previously located the predisposing genetic defect to the long arm of chromosome 11 by genetic linkage. In this study, 124 members of six MEN1 families, including 59 affected individuals, were genotyped for restriction fragment length polymorphisms with different DNA probes, and the genetic linkage between these marker systems and MEN1 was determined. 13 marker systems (17 DNA probes) were found to be linked to MEN1. These markers are located within a region on chromosome 11 spanning 14% meiotic recombinations, with the MEN1 locus in the middle. Four of the marker systems are on the centromeric side of MEN1, and four on the telomeric side, based on meiotic crossovers. The remaining five DNA probes are closely linked to MEN1, with no crossovers in our set of families. The 13 marker systems can be used for an accurate and reliable premorbid test for MEN1. In most clinical situations it is possible to identify a haplotype of this part of chromosome 11 with the mutant MEN1 allele in the middle. The calculated predictive accuracy is greater than 99.5% if three such marker systems are informative. Therefore, genetic linkage testing can be used for informed genetic counseling in MEN1 families, and to avoid unnecessary biochemical screening programs.