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Citations to this article

Repeated glucose stimulation reveals distinct and lasting secretion patterns of individual rat pancreatic B cells.
E Giordano, … , V Cirulli, P Meda
E Giordano, … , V Cirulli, P Meda
Published June 1, 1991
Citation Information: J Clin Invest. 1991;87(6):2178-2185. https://doi.org/10.1172/JCI115251.
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Repeated glucose stimulation reveals distinct and lasting secretion patterns of individual rat pancreatic B cells.

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Abstract

To determine whether pancreatic B cells show a constant secretion pattern during repeated stimulations, we have used a sequential hemolytic plaque assay to monitor their individual insulin release during several successive 30-min incubations in the presence of 16.7 mM glucose. We have found that the total B cell secretion did not vary significantly in these successive glucose stimulations and that, under these conditions, the majority of B cells that were stimulated to release insulin during the first incubation also secreted during the second, third, and, when this was tested, during the fourth incubation. Similarly, most of the B cells that did not release detectable amounts of insulin during the first incubation did not secrete also during the two (or three) subsequent secretion tests. Together, the two groups of B cells that showed a constant secretory pattern, represented approximately 75% of the entire B cell population. The remaining 25% of B cells shifted from a secreting to a non-secreting state, or vice versa, from one incubation to another. These observations were made under three different time frames in which we tested single B cells as well as B cell clusters at rather different intervals. These findings support the existence of distinct B cell subpopulations differing lastingly in their ability to secrete insulin in response to glucose.

Authors

E Giordano, D Bosco, V Cirulli, P Meda

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Year: 2024 2023 2022 2021 2019 2017 2016 2015 2014 2013 2011 2008 2007 2006 2005 2003 2002 2000 1998 1997 1996 1995 1994 1993 Total
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Citations to this article (52)

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β-Cell Heterogeneity and Plasticity.
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Advances in anatomy, embryology, and cell biology 2024
Redox Status as a Key Driver of Healthy Pancreatic Beta-Cells.
Holendová B, Benáková Š, Křivonosková M, Plecitá-Hlavatá L
Physiological research 2024
A beta cell subset with enhanced insulin secretion and glucose metabolism is reduced in type 2 diabetes.
Rubio-Navarro A, Gómez-Banoy N, Stoll L, Dündar F, Mawla AM, Ma L, Cortada E, Zumbo P, Li A, Reiterer M, Montoya-Oviedo N, Homan EA, Imai N, Gilani A, Liu C, Naji A, Yang B, Chong ACN, Cohen DE, Chen S, Cao J, Pitt GS, Huising MO, Betel D, Lo JC
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Therapeutic potential of multifunctional myricetin for treatment of type 2 diabetes mellitus.
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Wnt4 is heterogeneously activated in maturing β-cells to control calcium signaling, metabolism and function.
Katsumoto K, Yennek S, Chen C, Silva LFD, Traikov S, Sever D, Azad A, Shan J, Vainio S, Ninov N, Speier S, Grapin-Botton A
Nature Communications 2022
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American journal of physiology. Endocrinology and metabolism 2021
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P Chabosseau, GA Rutter, SJ Millership
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KP Trogden, J Lee, KM Bracey, KH Ho, H McKinney, X Zhu, G Arpag, TG Folland, AB Osipovich, MA Magnuson, M Zanic, G Gu, WR Holmes, I Kaverina
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Human islets contain four distinct subtypes of β cells
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2008
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Isolated mouse pancreatic β-cells show cell-specific temporal response pattern
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Physiology and Pathophysiology of the Islets of Langerhans
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Heterogeneous secretion of individual B cells in response to D-glucose and to nonglucidic nutrient secretagogues
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GLUT-2 function in glucose-unresponsive beta cells of dexamethasone-induced diabetes in rats
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