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Citations to this article

Stimulation of rat endothelial cell transforming growth factor-beta production by bleomycin.
S H Phan, … , F Wolber, U S Ryan
S H Phan, … , F Wolber, U S Ryan
Published January 1, 1991
Citation Information: J Clin Invest. 1991;87(1):148-154. https://doi.org/10.1172/JCI114964.
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Research Article

Stimulation of rat endothelial cell transforming growth factor-beta production by bleomycin.

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Abstract

This study examines the hypothesis that mediators from lung endothelial cells could promote lung collagen synthesis in pulmonary fibrosis. Since bleomycin induces pulmonary fibrosis in humans and animals, the effects of this drug on endothelial cells were examined. Endothelial cell conditioned media were prepared in the presence of various doses of bleomycin, and tested for their ability to stimulate lung fibroblast collagen synthesis. The results show a dose-dependent stimulation of endothelial cell secretion of collagen synthesis stimulatory activity by bleomycin, which peaked at a dose greater than or equal to 100 ng/ml. Stimulation was selective for collagenous protein synthesis. Gel filtration analysis showed most of the activity to reside in fractions with an estimated molecular mass range of 10-27 kD. The activity was inhibited by anti-transforming growth factor-beta (TGF-beta)antibody, but not by nonimmune control IgG. The presence of TGF-beta was confirmed using the mink lung epithelial cell assay. Northern blotting revealed significant increases in TGF-beta mRNA in bleomycin-stimulated endothelial cells. Thus in vitro stimulation of endothelial cells by bleomycin upregulates TGF-beta production, presumably by increased transcription. In view of the chemotactic and matrix synthesis stimulatory properties of this cytokine, such an increase in TGF-beta production may play an important role in bleomycin-induced pulmonary fibrosis.

Authors

S H Phan, M Gharaee-Kermani, F Wolber, U S Ryan

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Total citations by year

Year: 2021 2020 2019 2017 2016 2014 2013 2011 2010 2009 2008 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 Total
Citations: 2 1 1 3 1 2 3 2 2 2 1 2 2 2 5 3 5 2 8 4 1 5 6 6 7 4 82
Citation information
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Citations to this article in year 2020 (1)

Title and authors Publication Year
Bleomycin: A novel osteogenesis inhibitor of dental follicle cells via a TGF‐β1/SMAD7/RUNX2 pathway
ZZ Li, HT Wang, GY Lee, Y Yang, YP Zou, B Wang, CJ Gong, Y Cai, JG Ren, JH Zhao
British Journal of Pharmacology 2020

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