The presence and properties of serum autoantibodies against beta-adrenergic receptors in patients with idiopathic dilated cardiomyopathy were studied using synthetic peptides derived from the predicted sequences of the human beta-adrenergic receptors. Peptides corresponding to the sequences of the second extracellular loop of the human beta 1- and beta 2-adrenergic receptors were used as antigens in an enzyme immunoassay to screen sera from patients with dilated cardiomyopathy (n = 42), ischemic heart disease (n = 17), or healthy blood donors (n = 34). The sera of thirteen dilated cardiomyopathy patients, none of the ischemic heart disease patients, and four of the healthy controls monospecifically recognized the beta 1-peptide. Only affinity-purified antibodies of these patients had a inhibitory effect on radioligand binding to the beta 1 receptor of C6 rat glioma cells. They recognized the receptor protein by immunoblot and bound in situ to human myocardial tissue. We conclude that a subgroup of patients with idiopathic dilated cardiomyopathy have in their sera autoantibodies specifically directed against the second extracellular loop of the beta 1-adrenergic receptor. These antibodies could serve as a marker of an autoimmune response with physiological and/or pathological implications.
Y Magnusson, S Marullo, S Hoyer, F Waagstein, B Andersson, A Vahlne, J G Guillet, A D Strosberg, A Hjalmarson, J Hoebeke
Title and authors | Publication | Year |
---|---|---|
Activating autoantibodies to the beta-1 adrenergic and m2 muscarinic receptors facilitate atrial fibrillation in patients with Graves' hyperthyroidism
S Stavrakis, X Yu, E Patterson, S Huang, SR Hamlett, L Chalmers, R Pappy, MW Cunningham, SA Morshed, TF Davies, R Lazzara, DC Kem |
Journal of the American College of Cardiology | 2009 |
Agonistic antibody to the α1-adrenergic receptor mobilizes intracellular calcium and induces phosphorylation of a cardiac 15-kDa protein
P Karczewski, H Haase, P Hempel, M Bimmler |
Molecular and Cellular Biochemistry | 2009 |
Detection of antibodies in cardiac autoimmunity
C Wehlou, JR Delanghe |
Clinica Chimica Acta | 2009 |
A Pilot Study on the Role of Autoantibody Targeting the β1-Adrenergic Receptor in the Response to β-blocker Therapy for Congestive Heart Failure
Y Nagatomo, T Yoshikawa, T Kohno, A Yoshizawa, A Baba, T Anzai, T Meguro, T Satoh, S Ogawa |
Journal of Cardiac Failure | 2009 |
Autoimmune Mechanisms Underlying Dilated Cardiomyopathy
T Yoshikawa, A Baba, Y Nagatomo |
Circulation Journal | 2009 |
The effects of autoantibodies against the second extracellular loop of α1-adrenoceptor on vasoconstriction
L Yan, Y Xu, H Yao, W Xue, J Tian, H Ren, Y Wu, G Yang, XL Ma, H Liu |
Basic Research in Cardiology | 2009 |