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Citations to this article

Elevated expression of transforming growth factor-beta and proteoglycan production in experimental glomerulonephritis. Possible role in expansion of the mesangial extracellular matrix.
S Okuda, … , E Ruoslahti, W A Border
S Okuda, … , E Ruoslahti, W A Border
Published August 1, 1990
Citation Information: J Clin Invest. 1990;86(2):453-462. https://doi.org/10.1172/JCI114731.
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Elevated expression of transforming growth factor-beta and proteoglycan production in experimental glomerulonephritis. Possible role in expansion of the mesangial extracellular matrix.

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Abstract

Glomerular accumulation of extracellular matrix is a prominent feature of progressive glomerulonephritis. Previously, we have shown that transforming growth factor-beta (TGF-beta) is unique among growth factors in regulating the production of the proteoglycans biglycan and decorin by glomerular mesangial cells in vitro. We now provide evidence of an elevated expression of TGF-beta, proteoglycans, and fibronectin in glomerulonephritis induced in rats by injection of anti-thymocyte serum (ATS). Glomeruli were cultured from rat kidneys at 1, 4, 7, 14, and 28 d after ATS administration. Increased proteoglycan synthesis was detected beginning on day 4, which peaked at a 4,900% increase compared with control on day 7, and returned toward control levels by day 28. The increased proteoglycan synthesis by cultured nephritic glomeruli, as well as that of fibronectin, were greatly reduced by addition of antiserum raised against a synthetic peptide from TGF-beta. Conditioned media from ATS glomerular cultures, when added to normal cultured mesangial cells, induced elevated proteoglycan synthesis that also peaked on day 7 and that mimicked the response to added exogenous TGF-beta. The stimulatory activity of the conditioned media was blocked by addition of TGF-beta antiserum. Prior addition of the immunizing peptide to the antiserum abolished the blocking effect. The main induced proteoglycans were identified as biglycan and decorin by immunoprecipitation with antiserum made against synthetic peptides from the proteoglycan core proteins. Glomerular histology showed mesangial matrix expansion in a time course that roughly paralleled both the elevated proteoglycan synthesis by the ATS glomeruli and the ability of the conditioned media from these glomeruli to induce proteoglycan synthesis. At the same time there was an increased expression of TGF-beta mRNA and TGF-beta protein in the glomeruli. These results suggest a central role for TGF-beta in the accumulation of pathological extracellular matrix in glomerulonephritis.

Authors

S Okuda, L R Languino, E Ruoslahti, W A Border

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1986 Total
Citations: 1 3 7 6 4 2 3 4 6 1 4 6 10 9 4 5 10 6 11 11 14 14 16 15 21 27 22 21 42 42 27 23 32 8 1 438
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Citations to this article in year 2006 (11)

Title and authors Publication Year
The suppressive effects of dietary protein restriction on the progression of renal impairment in OLETF rats
M Sakamoto, Y Akehi, G Mimura, M Tanaka, T Ohshita, M Yano, Y Ishimaru, J Ono
Clinical and Experimental Nephrology 2006
Carbonic-adsorbent AST-120 reduces overload of indoxyl sulfate and the plasma level of TGF-β1 in patients with chronic renal failure
S Iida, K Kohno, J Yoshimura, S Ueda, M Usui, H Miyazaki, H Nishida, K Tamaki, S Okuda
Clinical and Experimental Nephrology 2006
TGF-β1 mRNA upregulation influences chronic renal allograft dysfunction
P Pribylova-Hribova, K Kotsch, A Lodererova, O Viklicky, S Vitko, HD Volk, J Lacha
Kidney International 2006
Transcription factor Ets-1 is essential for mesangial matrix remodeling
M Mizui, Y Isaka, Y Takabatake, Y Sato, H Kawachi, F Shimizu, S Takahara, T Ito, E Imai
Kidney International 2006
Renin increases mesangial cell transforming growth factor-β1 and matrix proteins through receptor-mediated, angiotensin II-independent mechanisms
Y Huang, S Wongamorntham, J Kasting, D McQuillan, RT Owens, L Yu, NA Noble, W Border
Kidney International 2006
Noninhibitory PAI-1 enhances plasmin-mediated matrix degradation both in vitro and in experimental nephritis
Y Huang, WA Border, DA Lawrence, NA Noble
Kidney International 2006
Honokiol, a small molecular weight natural product, alleviates experimental mesangial proliferative glomerulonephritis
CK Chiang, ML Sheu, KY Hung, KD Wu, SH Liu
Kidney International 2006
Enhanced anti-fibrotic activity of plasmid DNA expressing small interference RNA for TGF-β type II receptor for a mouse model of obstructive nephropathy by cationized gelatin prepared from different amine compounds
T Kushibiki, N Nagata-Nakajima, M Sugai, A Shimizu, Y Tabata
Journal of Controlled Release 2006
TGF-β1 siRNA suppresses the tubulointerstitial fibrosis in the kidney of ureteral obstruction
M Hwang, HJ Kim, HJ Noh, YC Chang, YM Chae, KH Kim, JP Jeon, TS Lee, HK Oh, YS Lee, KK Park
Experimental and Molecular Pathology 2006
Attenuating Effects of Wen-Pi-Tang Treatment in Rats with Diabetic Nephropathy
T Yokozawa, A Satoh, T Nakagawa, N Yamabe
The American journal of Chinese medicine 2006
Role of fibrillin-1 in hypertensive and diabetic glomerular disease
A Hartner, L Schaefer, M Porst, N Cordasic, A Gabriel, B Klanke, DP Reinhardt, KF Hilgers
American journal of physiology. Renal physiology 2006

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