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Research Article Free access | 10.1172/JCI114581

Vitronectin binds to activated human platelets and plays a role in platelet aggregation.

E Asch and E Podack

Division of Rheumatic Diseases and Immunology, New York Medical College, Valhalla 10595.

Find articles by Asch, E. in: JCI | PubMed | Google Scholar

Division of Rheumatic Diseases and Immunology, New York Medical College, Valhalla 10595.

Find articles by Podack, E. in: JCI | PubMed | Google Scholar

Published May 1, 1990 - More info

Published in Volume 85, Issue 5 on May 1, 1990
J Clin Invest. 1990;85(5):1372–1378. https://doi.org/10.1172/JCI114581.
© 1990 The American Society for Clinical Investigation
Published May 1, 1990 - Version history
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Abstract

Vitronectin (Vn) is a multifunctional 75-kD glycoprotein that is present in plasma and the extracellular matrix. Vn functions as a complement regulatory protein in plasma, and promotes the growth and attachment of cells in tissue culture. Recent cDNA cloning reveals that like other adhesive proteins, Vn contains the sequence Arg-Gly-Asp and binds to some members of the integrin class of adhesive membrane receptors. In liposomes, the platelet membrane glycoprotein complex IIb/IIIa binds Vn, as well as fibrinogen, von Willebrand factor, and fibronectin. We examined the binding of purified Vn to resting and stimulated human platelets. Vn bound to thrombin-stimulated platelets in a calcium-dependent, specific, and saturable manner with a Kd of 320 nM and 8,000 sites per platelet. Epinephrine or ADP stimulation led to specific binding with KdS of 93 and 116 nM, respectively. Binding was inhibited by the tetrapeptide Arg-Gly-Asp-Ser and by monoclonal and polyclonal antibodies to GPIIb/IIIa. Endogenous platelet Vn stores were identified in immunoblots of gel-filtered platelets and the surface expression of endogenous platelet Vn was thrombin inducible. Monoclonal as well as polyclonal antibodies to Vn inhibited platelet aggregation, suggesting that Vn plays a role in the formation of stable platelet aggregates.

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Referenced in 1 patents
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