C.B-17 scid mice were reconstituted by intraperitoneal injection of human tonsil cells or PBL from EBV-seronegative donors. Subsequent injection of EBV resulted in the rapid development (within 19-33 d) of aggressive, fatal, lymphoproliferative disorders of human B cell origin. Autopsies revealed solid tumors in the abdomen, and occasionally in the liver, thymus, or spleen. Histopathologic analysis showed that the tumors were high-grade immunoblastic lymphomas and FACS analyses of tumor cells indicated that they were of human B-lymphoid origin. The tumor cells grew in vitro and induced new tumors on injection into severe combined immunodeficient (SCID) mice. Karyotypic analysis and Southern blots for c-myc or bcl-2 rearrangements revealed no chromosomal abnormalities and translocations. Southern blot analysis also showed that the cells possessed EBV DNA sequences. Although these tumors undoubtedly reflect infection of the transferred B cells with EBV in vivo, intraperitoneal transfer of short-term lymphoid cell lines transformed in vitro with EBV resulted in ascites production without evidence of tumor formation.
M J Cannon, P Pisa, R I Fox, N R Cooper
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Morphologic and immunophenotypic characterization of a cell line derived from liver tissue with epstein-barr virus associated post-transplant lymphoproliferative disease
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The American Journal of Pathology | 1993 |
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The American Journal of Pathology | 1993 |
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Proceedings of the National Academy of Sciences | 1993 |
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The American Journal of Pathology | 1993 |
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A study on the engraftment and trafficking of bovine peripheral blood leukocytes in severe combined immunodeficient mice
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Veterinary Immunology and Immunopathology | 1993 |
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1993 | |
Control of human B cell tumor growth in severe combined immunodeficiency mice by monoclonal anti-B cell antibodies
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Analysis of autoantibody production in SCID-systemic lupus erythematosus (SLE) chimeras
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Current Opinion in Immunology | 1991 |
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Cancer | 1991 |
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Current Opinion in Immunology | 1991 |
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The American Journal of Medicine | 1991 |
Molecular Genetic Medicine
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Cancer in Organ Transplant Recipients
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1991 | |
Potential of the scid mouse as a host for human tumors
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Search for the critical characteristics of phenotypically different B cell lines, Burkitt lymphoma cells and lymphoblastoid cell lines, which determine differences in their functional interaction with allogeneic lymphocytes
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Cancer Immunology, Immunotherapy | 1991 |
Characterization of Epstein-Barr virus-induced lymphoproliferation derived from human peripheral blood mononuclear cells transferred to severe combined immunodeficient mice
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