Citations to this article

Identification of a satellite fatty acid ethyl ester synthase from human myocardium as a glutathione S-transferase.
P S Bora, … , C A Spilburg, L G Lange
P S Bora, … , C A Spilburg, L G Lange
Published December 1, 1989
Citation Information: J Clin Invest. 1989;84(6):1942-1946. https://doi.org/10.1172/JCI114382.
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Research Article

Identification of a satellite fatty acid ethyl ester synthase from human myocardium as a glutathione S-transferase.

Abstract

Nonoxidative alcohol metabolism catalyzed by fatty acid ethyl ester (FAEE) synthases may contribute to extrahepatic injury resulting from alcohol abuse. Unlike rabbit myocardial FAEE synthase, that from human heart has a satellite minor synthase (I) eluting from DEAE cellulose at a conductivity of 5 mS. Synthase I was purified 1,118-fold to homogeneity by sequential gel permeation, hydrophobic interaction, and Superose-12 fast-protein liquid chromatographies. SDS-PAGE showed a single polypeptide with a molecular mass of 26 kD and gel permeation chromatography indicated a molecular mass of 52 kD for the active enzyme. Homogeneous synthase I catalyzed ethyl ester synthesis at highest rates with unsaturated octadecanoic fatty acid substrates. The amino acid composition of synthase I was highly homologous to that of human myocardial major synthase, recently identified as an acidic glutathione (GSH) S-transferase. Antibody raised against homogeneous human heart major synthase cross-reacted with the 26-kD synthase I. FAEE synthase co-chromatographed with GSH S-transferase on DEAE cellulose, Sephadex G-100 and S-hexylglutathione agarose, and also displayed GSH S-transferase activity in catalyzing the conjugation of GSH with nitrobenzene-containing carcinogens. Thus, human myocardium contains a satellite peak of FAEE synthase activity and it is a neutral GSH S-transferase.

Authors

P S Bora, C A Spilburg, L G Lange

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Citations to this article (33)

Title and authors Publication Year
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IF Kodde, J der Stok, RT Smolenski, JW de Jong 		Comparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 2007
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J Sastre, JB Miñana, FV Pallardó, J Viña 		Comprehensive Handbook of Alcohol Related Pathology 2004
Molecular characterization of human eye and heart fatty acid ethyl ester synthase/carboxylesterase by site-directed mutagenesis
PS Bora, BL Guruge, NS Bora 		Biochemical and Biophysical Research Communications 2003
Effects of L-carnitine on the formation of fatty acid ethyl esters in brain and peripheral organs after short-term ethanol administration in rat
V Calabrese, V Rizza 		Neurochemical Research 1999
Fatty acid ethyl esters: ethanol metabolites which mediate ethanol-induced organ damage and serve as markers of ethanol intake
M Laposata 		Progress in Lipid Research 1998
Fatty acid ethyl esters: nonoxidative metabolites of ethanol
M Laposata 		Addiction Biology 1998
Cardiac Metabolism in Health and Disease
S Abdel-Aleem, JE Lowe 		1998
Fatty acid ethyl esters and HepG2 cells: intracellular synthesis and release from the cells
A Kabakibi, CR Morse, M Laposata 		Journal of lipid research 1998
Mutagenesis and characterization of specific residues in fatty acid ethyl ester synthase: a gene for alcohol-induced cardiomyopathy
P S Bora, D D Miller, B R Chaitman 		Molecular and Cellular Biochemistry 1998
Fatty acid ethyl ester synthesis by the isolated perfused rat heart
W Chang, C Waltenbaugh, J Borensztajn 		Metabolism 1997
Human fatty acid ethyl ester synthase-III gene: genomic organization, nucleotide sequencing and chromosomal localization
P S Bora, B L Guruge, D D Miller, B R Chaitman, W Fortson 		Molecular and Cellular Biochemistry 1997
Myocardial Cell Damage by Fatty Acid Ethyl Esters
PS Bora, MA Farrar, DD Miller, BR Chaitman, BL Guruge 		Journal of Cardiovascular Pharmacology 1996
Identification of fatty acid methyl ester as naturally occurring transcriptional regulators of the members of the peroxisome proliferator-activated receptor family
A Schmidt, RL Vogel, KM Witherup, SJ Rutledge, SM Pitzenberger, M Adam, GA Rodan 		Lipids 1996
Fatty acid ethyl ester synthesis by human liver microsomes
T Treloar 		Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1996
Fatty acid ethyl esters decrease human hepatoblastoma cell proliferation and protein synthesis
ZM Szczepiorkowski, GR Dickersin, M Laposata 		Gastroenterology 1995
Comparison of the induction of rat glutathione S-transferase and fatty acid ethyl ester synthase activities
GP Carlson, AM Chaney, F Jiang 		Toxicology 1995
Fatty acid conjugates of xenobiotics
GA Ansari, BS Kaphalia, MF Khan 		Toxicology Letters 1995
Advances in Pharmacology
B Drenger, Y Ginosar, Y Gozal 		Advances in Pharmacology 1994
Molecular Mechanism of Ethanol Metabolism by Human Brain to Fatty Acid Ethyl Esters
PS Bora, LG Lange 		Alcoholism, clinical and experimental research 1993
Evidence against a relationship between fatty acid ethyl ester synthase and the Pi class glutathione S-transferase in humans
P Board, S Smith, J Green, M Coggan, T Suzuki 		The Journal of biological chemistry 1993
Nonoxidative ethanol metabolism: Expression of fatty acid ethyl ester synthase-III in cultured neural cells
KE Isenberg, PS Bora, X Zhou, X Wu, BW Moore, LG Lange 		Biochemical and Biophysical Research Communications 1992
Site-specific mutagenesis of two histidine residues in fatty acid ethyl ester synthase-III
PS Bora, X Wu, LG Lange 		Biochemical and Biophysical Research Communications 1992
Purification and characterization of fatty acid ethyl ester synthase-II from human myocardium
PS Bora, X Wu, CA Spilburg, LG Lange 		The Journal of biological chemistry 1992
Mechanism of Fatty Acid Ethyl Ester Formation and Biological Significance
LG Lange 		Annals of the New York Academy of Sciences 1991
Homogeneous Synthase I from Human Myocardium is a Glutathione S-Transferase
PS Bora, LG Lange 		Annals of the New York Academy of Sciences 1991
Independent segregation of glutathione S-transferase and fatty acid ethyl ester synthase from pancreas and other human tissues
R Sharma, S Gupta, SS Singhal, H Ahmad, A Haque, YC Awasthi 		Biochemical Journal 1991
Purification and characterization of glutathione S-transferases from rat pancreas
SS Singhal, S Gupta, M Saxena, R Sharma, H Ahmad, GA Ansari, YC Awasthi 		Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1991
Liver Pathology and Alcohol
RR Watson 		1991
The Genetic Basis of Alcohol and Drug Actions
JC Crabbe, RA Harris 		1991
Molecular cloning, sequencing, and expression of human myocardial fatty acid ethyl ester synthase-III cDNA
PS Bora, NS Bora, XL Wu, LG Lange 		The Journal of biological chemistry 1991
Do the major human glutathione S-transferases have fatty acid ethyl ester synthase activity?
T Suzuki, MS Kovacs, PG Board 		FEBS Letters 1990

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