Citations to this article

Complement induces a transient increase in membrane permeability in unlysed erythrocytes.
J A Halperin, … , C Brugnara, D C Tosteson
J A Halperin, … , C Brugnara, D C Tosteson
Published August 1, 1988
Citation Information: J Clin Invest. 1988;82(2):594-600. https://doi.org/10.1172/JCI113637.
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Research Article

Complement induces a transient increase in membrane permeability in unlysed erythrocytes.

Abstract

The effects of low concentrations of human serum on antibody-sensitized sheep erythrocytes (EA) were studied. We report that exposure to low concentrations of serum induced a large but transient increase in the membrane permeability of those EA that do not lyse. This change in the permeability of the erythrocyte membrane resulted in net uptake of Na+ and decrease in cell K+, without affecting the total internal cation content. Although exposure to serum also allowed for net uptake of larger molecules like L-glucose, it did not lead to cell swelling. Experiments with sera genetically deficient in one of the terminal complement components showed that C8, but not C9, was required to produce the observed change in membrane permeability. Therefore, we propose that the C5b-8 complex can mediate the transient increase in permeability observed in unlysed erythrocytes during complement activation by whole serum.

Authors

J A Halperin, A Nicholson-Weller, C Brugnara, D C Tosteson

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Citations to this article (25)

Title and authors Publication Year
Incident microalbuminuria and complement factor mannan-binding lectin-associated protein 19 in people with newly diagnosed type 1 diabetes
JA Østergaard, S Thiel, IT Hoffmann-Petersen, P Hovind, HH Parving, L Tarnow, P Rossing, TK Hansen 		Diabetes/Metabolism Research and Reviews 2017
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C Yang, P Ding, Q Wang, L Zhang, X Zhang, J Zhao, E Xu, N Wang, J Chen, G Yang, W Hu, X Zhou 		Scientific Reports 2016
Role of Complement and Complement Regulatory Proteins in the Complications of Diabetes
P Ghosh, R Sahoo, A Vaidya, M Chorev, JA Halperin 		Endocrine reviews 2015
Inflammatory Pathways in Diabetes
M Pugia 		2015
Association of the pattern recognition molecule H-ficolin with incident microalbuminuria in an inception cohort of newly diagnosed type 1 diabetic patients: an 18 year follow-up study
JA Østergaard, S Thiel, P Hovind, CB Holt, HH Parving, A Flyvbjerg, P Rossing, TK Hansen 		Diabetologia 2014
Activation of endogenously expressed ion channels by active complement in the retinal pigment epithelium
A Genewsky, I Jost, C Busch, C Huber, J Stindl, C Skerka, PF Zipfel, B Rohrer, O Strauß 		Pflügers Archiv - European Journal of Physiology 2014
Evidence for a protective role of the Gardos channel against hemolysis in murine spherocytosis
LD Franceschi, A Rivera, MD Fleming, M Honczarenko, LL Peters, P Gascard, N Mohandas, C Brugnara 		Blood 2005
Complement activation alters myocellular sodium homeostasis during polymicrobial sepsis
W Wang, K Okamoto, DO Jacobs 		Critical Care Medicine 2002
Does Endotoxin-Activated Complement Alter Myocellular Sodium Homeostasis during Sepsis?:
W Wang, K Okamoto, DO Jacobs 		The Journal of trauma 2002
Changes in Erythrocyte Ca 2+ , K + , and Mg 2+ Content in Uremic Patients on CAPD
B Agroyannis, C Fourtounas, I Kopelias, A Paraskevopoulos, G Bougatsos, S Katsoudas, H Tzanatos, A Tsomi, S Voudiclari 		Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis 2002
Increased susceptibility to erythrocyte C5b-9 deposition and complement-mediated lysis in chronic renal failure
J Himmelfarb, E Mcmonagle, D Holbrook, R Hakim 		Kidney International 1999
Characterization of neuronal cell death induced by complement activation
Y Shen, JA Halperin, L Benzaquen, CM Lee 		Brain research. Brain research protocols 1997
The transient pore formed by homologous terminal complement complexes functions as a bidirectional route for the transport of autocrine and paracrine signals across human cell membranes
JA Acosta, LR Benzaquen, DJ Goldstein, MT Tosteson, JA Halperin 		Molecular Medicine 1996
Complement-mediated neurotoxicity is regulated by homologous restriction
Y Shen, JA Halperin, CM Lee 		Brain Research 1995
Terminal complement proteins C5b-9 release basic fibroblast growth factor and platelet-derived growth factor from endothelial cells
LR Benzaquen, A Nicholson-Weller, JA Halperin 		Journal of Experimental Medicine 1994
Terminal complement complex C5b-9 stimulates mitogenesis in 3T3 cells
JA Halperin, A Taratuska, A Nicholson-Weller 		Journal of Clinical Investigation 1993
Membrane signaling by complement C5b-9, the membrane attack complex
A Nicholson-Weller, JA Halperin 		Immunologic Research 1993
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GW Stewart 		Baillière's Clinical Haematology 1993
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A Agostoni, M Cicardi, M Gardinali, L Bergamaschini 		International journal of immunopathology and pharmacology 1992
Red cell deformability and haematological disorders
J Stuart, GB Nash 		Blood Reviews 1990
Functions and relevance of the terminal complement sequence
S Bhakdi, F Hugo, J Tranum-Jensen 		Blut 1990
Functions and relevance of the terminal complement sequence
S Bhakdi, F Hugo, J Tranum-Jensen 		Blut Zeitschrift für die Gesamte Blutforschung 1990
Ca2+-activated K+ efflux limits complement-mediated lysis of human erythrocytes
JA Halperin, C Brugnara, A Nicholson-Weller 		Journal of Clinical Investigation 1989
Detection of a high-affinity binding site for the globular head regions of the C1q complement protein on a human diploid fibroblast subtype
S Bordin, RC Page 		Molecular Immunology 1989
Digitalis-like properties of an inhibitor of the pump in human cerebrospinal fluid
JA Halperin 		Journal of the Neurological Sciences 1989

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