Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Increased leukotriene B4 synthesis in immune injured rat glomeruli.
M A Rahman, … , S N Emancipator, M J Dunn
M A Rahman, … , S N Emancipator, M J Dunn
Published June 1, 1988
Citation Information: J Clin Invest. 1988;81(6):1945-1952. https://doi.org/10.1172/JCI113542.
View: Text | PDF
Research Article

Increased leukotriene B4 synthesis in immune injured rat glomeruli.

  • Text
  • PDF
Abstract

We examined glomerular synthesis of the 5-lipoxygenase metabolite, LTB4, in normal and immune-injured rat glomeruli. Glomeruli isolated from normal rats and from rats with nephrotoxic serum nephritis (NSN), passive Heymann nephritis (PHN) and cationic bovine gamma globulin (CBGG)-induced glomerulonephritis were incubated with the calcium ionophore A23187 (3 microM). Lipids in the glomeruli and media were extracted with ethyl acetate, and were purified and fractionated by HPLC. Immunoreactive-LTB4 (i-LTB4) was determined by radioimmunoassay on HPLC fractions with a detection limit of 50 pg of i-LTB4. A large peak of i-LTB4 that comigrated with authentic LTB4 was found exclusively in glomeruli isolated from the CBGG-injected rats. Addition of the lipoxygenase inhibitor BW755C (50 micrograms/ml) to glomerular incubation resulted in greater than 90% inhibition of i-LTB4. Synthesis of i-LTB4 by glomeruli from normal, NSN and PHN rats was undetectable. Glomerular LTB4 synthesis by CBGG-injected rats was confirmed by radiometric HPLC and by gas chromatography mass-spectroscopy (GC-MS) analysis. In order to rule out synthesis of LTB4 by neutrophils entrapped in the glomeruli, a group of rats received 1,000 rad total body x irradiation, with shielding of the kidneys before induction of CBGG glomerulonephritis. Despite greater than 95% reduction in total leukocyte count, glomerular synthesis of LTB4 remained enhanced. Augmented glomerular synthesis of the proinflammatory lipid, LTB4, in the CBGG model of glomerular disease could have an important role in the development of glomerular injury and proteinuria.

Authors

M A Rahman, M Nakazawa, S N Emancipator, M J Dunn

×

Total citations by year

Year: 2012 2011 2004 2003 2002 2001 2000 1999 1996 1995 1994 1993 1992 1991 1990 1989 1987 Total
Citations: 2 2 1 1 1 1 2 4 1 1 1 4 2 7 5 3 1 39
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (39)

Title and authors Publication Year
5-Lipoxygenase Metabolic Contributions to NSAID-Induced Organ Toxicity
BP Burnett, RM Levy
Advances in Therapy 2012
Brenner and Rector's The Kidney
C Brugnara, KU Eckardt
Brenner and Rector's The Kidney 2012
Role of leukotriene B4 in accelerated hyperlipidaemic renal injury
K Doi, Y Hamasaki, E Noiri, K Nosaka, T Suzuki, A Toda, T Shimizu, T Fujita, A Nakao
Nephrology 2011
Annual Reports in Medicinal Chemistry
JJ Liu, TW Lee
Annual Reports in Medicinal Chemistry Volume 46 2011
5-Lipoxygenase and leukotriene A 4 hydrolase expression in primary nephrotic syndrome
A Bakr, S Hawas, S Slem, AA Moniem, T Ghatab, M Tawfik
Pediatric Nephrology 2004
Detrimental Effects of a High Fat Diet in Early Renal Injury Are Ameliorated by Fish Oil in Han:SPRD-cy Rats
J Lu, N Bankovic-Calic, M Ogborn, MH Saboorian, HM Aukema
The Journal of nutrition 2003
Induction of Nephrotoxic Serum Nephritis in Inbred Mice and Suppressive Effect of Colchicine on the Development of this Nephritis
S Chen, T Mukoyama, N Sato, SI Yamagata, Y Arai, N Satoh, S Ueda
Pharmacological Research 2002
Immunohistochemical localization of guinea-pig leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin 13-reductase
T Yamamoto, T Yokomizo, A Nakao, T Izumi, T Shimizu
European Journal of Biochemistry 2001
Leukotriene B 4 Receptor: Cloning and Intracellular Signaling
T Yokomizo, K Masuda, K Kato, A Toda, T Izumi, T Shimizu
American journal of respiratory and critical care medicine 2000
Advances in Eicosanoid Research
CN Serhan, HD Perez
2000
Ubiquitous localization of leukotriene A4 hydrolase in the rat nephron
A Nakao, T Watanabe, N Ohishi, A Toda, K Asano, S Taniguchi, K Nosaka, E Noiri, T Suzuki, T Sakai, K Kurokawa, T Shimizu, S Kimura
Kidney International 1999
Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis
KA Munger, A Montero, M Fukunaga, S Uda, T Yura, E Imai, Y Kaneda, JM Valdivielso, KF Badr
Proceedings of the National Academy of Sciences 1999
Effect of Estradiol and Progesterone on the Low-Dose Endotoxin-Induced Glomerular Inflammatory Response of the Female Rat
MM Faas, WW Bakker, N Valkhof, GA Schuiling
American Journal of Reproductive Immunology 1999
The Leukotriene B4 Receptor Antagonist ONO-4057 Inhibits Nephrotoxic Serum Nephritis in WKY Rats
S Suzuki, T Kuroda, JI Kazama, N Imai, H Kimura, M Arakawa, F Gejyo
Journal of the American Society of Nephrology : JASN 1999
Cytokines, Growth Factors, and Other Inflammatory Mediators in Glomerulonephritis
A Papayianni
Renal Failure 1996
Leukotriene A4 hydrolase and leukotriene B4 metabolism
T Yokomizo, N Uozumi, T Takahashi, K Kume, T Izumi, T Shimizu
Journal of Lipid Mediators and Cell Signalling 1995
Lipoxygenase Products in Normal and Diseased Glomeruli
KF Badr, FG Lakkis
Annals of the New York Academy of Sciences 1994
Renal complications of nonsteroidal anti-inflammatory drugs
D Schlondorff
Kidney International 1993
Phospholipid derived mediators and glomerulonephritis
EN Wardle
Mediators of Inflammation 1993
Rat mesangial cell lysis in vitro is induced by cationic polypeptides
JA Broestl, SN Emancipator
The American Journal of Pathology 1993
Enzymatic inactivation of leukotriene B4 by a novel enzyme found in the porcine kidney. Purification and properties of leukotriene B4 12-hydroxydehydrogenase
T Yokomizo, T Izumi, T Takahashi, T Kasama, Y Kobayashi, F Sato, Y Taketani, T Shimizu
The Journal of biological chemistry 1993
Role of the 5-lipooxygenase pathway in obstructive nephropathy
AA Reyes, J Lefkowith, J Pippin, S Klahr
Kidney International 1992
Fifteen-S-hydroxyeicosatetraenoic acid (15-S-HETE) specifically antagonizes the chemotactic action and glomerular synthesis of leukotriene B4 in the rat
DB Fischer, JW Christman, KF Badr
Kidney International 1992
Mesangial cell immune injury. Synthesis, origin, and role of eicosanoids
EA Lianos, BA Bresnahan, C Pan
Journal of Clinical Investigation 1991
Enhanced renal leukotriene production in murine lupus: Role of lipoxygenase metabolites
RF Spurney, P Ruiz, DS Pisetsky, TM Coffman
Kidney International 1991
Involvement of Lipid Mediators in the Pathogenesis of Experimental Nephrosis in Rats: Its Pharmacological Modulation
J Egido, A Ortiz, M Goméz-Chiarri, JL Lerma, A Robles, C Bustos, C Gómez-Guerrero, J Alonso, E González
Renal Failure 1991
Involvement of thromboxane A2, leukotrienes and free radicals in puromycin nephrosis in rats
Y Shibouta, Z Terashita, Y Imura, A Shino, M Kawamura, K Ohtsuki, S Ohkawa, K Nishikawa, Y Fujiwara
Kidney International 1991
Lipoxygenases and their Products
MA Wasserman, EF Smith, DC Underwood, MA Barnette
Lipoxygenases and their Products 1991
Cell-Cell Interactions in the Release of Inflammatory Mediators
PY Wong, CN Serhan
1991
Immunology of Renal Disease
CD Pusey
1991
Predominant functional roles for thromboxane A2 and prostaglandin E2 during late nephrotoxic serum glomerulonephritis in the rat
K Takahashi, GF Schreiner, K Yamashita, BW Christman, I Blair, KF Badr
Journal of Clinical Investigation 1990
Endothelial factors in the regulation of renin release
WB Campbell, WL Henrich
Kidney International 1990
Eicosanoid biosynthesis and role in renal immune injury
EA Lianos
Prostaglandins, Leukotrienes and Essential Fatty Acids 1990
Human glomerular mesangial cells inactivate leukotriene B4 by reduction into dihydro-leukotriene B4 metabolites
V Kaever, J Bruuns, J Wunder, B Damerau, G Zimmer, J Fauler, K Wessel, J Floege, N Topley, H Radeke, K Resch
Life Sciences 1990
Leukotriene B4 production in children with steroid-responsive nephrotic syndrome
Y Kikawa, A Nakai, Y Shigematsu, M Sudo, K Kato, S Haruki
Pediatric Nephrology 1990
Eicosanoids and the modulation of glomerular immune injury
EA Lianos
Kidney International 1989
Glomerular leukotriene synthesis in Heymann nephritis
EA Lianos, B Noble, B Hucke
Kidney International 1989
LTB4 in nephrotoxic serum nephritis in rats
J Fauler, A Wiemeyer, KH Marx, K Kühn, KM Koch, JC Frölich
Kidney International 1989
Role of arachidonic acid metabolites and reactive oxygen species in glomerular immune-inflammatory process
R Ardaillou, L Baud, J Sraer
Springer Seminars in Immunopathology 1987

← Previous 1 2 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts