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Abstract
We investigated the effects of estradiol on bioactive luteinizing hormone (LH) release in normal men using two complementary strategies: (i) steady state intravenous infusions of estradiol at its endogenous production rate, and (ii) oral administration of the antiestrogen, tamoxifen HCl. Immunoreactive and biologically active LH were monitored by radioimmunoassay and the rat interstitial cell testosterone bioassay, respectively. Estradiol infusions significantly suppressed mean plasma bioactive LH concentrations and decreased the bio/immuno LH ratio. Conversely, antiestrogen treatment enhanced spontaneous bioactive LH pulse frequency, increased bioactive LH pulse amplitude, and augmented plasma intrapulse and interpulse bio/immuno LH ratios. Low-dose pulsed injections of exogenous gonadotropin-releasing hormone (GnRH) also increased plasma bio/immuno LH ratios. However, tamoxifen attenuated the ability of exogenous GnRH to further enhance the bio/immuno LH ratio, which suggests that endogenous LH release was already maximally enriched in LH bioactivity during antiestrogen administration. We conclude that estradiol modulates the pulsatile secretion of LH molecules enriched in biological activity in man.
Authors
J D Veldhuis, M L Dufau
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| Title and authors | Publication | Year |
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Controversies in Testosterone Deficiency
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MRI findings of nonobstructive azoospermia: lesions in and out of pelvic cavity
Y Peng, L Ouyang, Z Lin, F Zhang, H Wang, J Guan |
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Male Hypogonadism
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Male Hypogonadism | 2017 |
|
Role of Estrogen Receptors and G Protein-Coupled Estrogen Receptor in Regulation of Hypothalamus-Pituitary-Testis Axis and Spermatogenesis
A Chimento, R Sirianni, I Casaburi, V Pezzi |
Frontiers in Endocrinology | 2014 |
|
Anabolic steroid–induced hypogonadism: diagnosis and treatment
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Associations between visceral adipose tissue, inflammation and sex steroid concentrations in men
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Cross-Talk between Metabolism and Reproduction: The Role of POMC and SF1 Neurons
Y Xu, LD Faulkner, JW Hill |
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|
Functional relationship between obesity and male reproduction: from humans to animal models
KJ Teerds, DG de Rooij, J Keijer |
Human Reproduction Update | 2011 |
|
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|
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American journal of physiology. Endocrinology and metabolism | 2010 |
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Effects of Aromatase Inhibition on Bone Mineral Density and Bone Turnover in Older Men with Low Testosterone Levels
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|
Yen & Jaffe's Reproductive Endocrinology
RA Lobo |
Yen & Jaffe's Reproductive Endocrinology | 2009 |
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Effects of aromatase inhibition in hypogonadal older men: a randomized, double-blind, placebo-controlled trial
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|
Yen & Jaffe's Reproductive Endocrinology
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Yen & Jaffe's Reproductive Endocrinology | 2009 |
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Anabolic steroid-induced hypogonadism – Towards a unified hypothesis of anabolic steroid action
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Interplay between Dose and Frequency of GnRH Administration in Determining Pituitary Gonadotropin Responsiveness
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|
Obesity
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Obesity | 2007 |
|
In vitro biological-to-immunological ratio of serum gonadotropins throughout male puberty in children with insulin-dependent diabetes mellitus
E Nishimura, D Söderlund, C Castro-Fernández, T Zariñán, JP Méndez, A Ulloa-Aguirre |
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Testosterone, estradiol and aromatase inhibitor therapy in elderly men
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|
Effects of Aromatase Inhibition in Elderly Men with Low or Borderline-Low Serum Testosterone Levels
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|
Five-Day Pulsatile Gonadotropin-Releasing Hormone Administration Unveils Combined Hypothalamic-Pituitary-Gonadal Defects Underlying Profound Hypoandrogenism in Men with Prolonged Critical Illness1
GV den Berghe, F Weekers, RC Baxter, P Wouters, A Iranmanesh, R Bouillon, JD Veldhuis |
The Journal of clinical endocrinology and metabolism | 2001 |
|
Aromatase Inhibition in the Human Male Reveals a Hypothalamic Site of Estrogen Feedback*
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The Journal of clinical endocrinology and metabolism | 2000 |
|
Leptin and Androgens in Male Obesity: Evidence for Leptin Contribution to Reduced Androgen Levels*
AM Isidori, M Caprio, F Strollo, C Moretti, G Frajese, A Isidori, A Fabbri |
The Journal of clinical endocrinology and metabolism | 1999 |
|
Prenatal exposure of female rhesus monkeys to testosterone propionate increases serum luteinizing hormone levels in adulthood
DA Dumesic, DH Abbott, JR Eisner, RW Goy |
Fertility and Sterility | 1997 |
|
Neuroendocrine mechanisms mediating awakening of the human gonadotropic axis in puberty
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|
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Systems Biology in Reproductive Medicine | 1996 |
|
Estrogen and progesterone receptor mRNA are expressed in distinct pattern in male primate reproductive organs
O Heikinheimo, MC Mahony, K Gordon, JG Hsiu, GD Hodgen, WE Gibbons |
Journal of Assisted Reproduction and Genetics | 1995 |
|
Estrogen and progesterone receptor mRNA are expressed in distinct pattern in male primate reproductive organs
O Heikinheimo, MC Mahony, K Gordon, , GD Hodgen, WE Gibbons |
Journal of Assisted Reproduction and Genetics | 1995 |
|
Effects of oestrogen treatment on serum gonadotrophin bioactivity, immunoreactivity and isohormone distribution, and on immunoreactive inhibin levels, in prostatic cancer patients
T Matikainen, AM Haavisto, J Permi, D Kretser, I Huhtaniemi |
Clinical Endocrinology | 1994 |
|
1 The differential effects of FSH and LH on the human ovary
J Rabinovici |
Baillière's Clinical Obstetrics and Gynaecology | 1993 |
|
Ovarial steroids and methionine-enkephalin modulation of adrenergic transmission in rabbit oviduct
L Chernaeva, Z Mizhorkova |
General Pharmacology: The Vascular System | 1993 |
|
Neuroendocrine Physiology of the Normal Male Gonadal Axis
A Reyes-Fuentes, JD Veldhuis |
Endocrinology and metabolism clinics of North America | 1993 |
|
Effects of ethinyl estradiol on semen quality and various hormonal parameters in a eugonadal male
H Lübbert, I Leo-Roßberg, J Hammerstein |
Fertility and Sterility | 1992 |
|
Biologic Rhythms in Clinical and Laboratory Medicine
Y Touitou, E Haus |
1992 | |
|
Bioactive gonadotrophin secretion in man
A Tsatsoulla, SM Shalet, WR Robertson |
Clinical Endocrinology | 1991 |
|
TESTOSTERONE REDUCES THE BIOACTIVITY OF LUTEINIZING HORMONE (LH) IN MAN
A Tsatsoulis, SM Shalet, P Richardson, WR Robertson |
Clinical Endocrinology | 1990 |
|
Neuroendocrine regulation of pulsatile luteinizing hormone secretion in elderly men
JM Kaufman, JP Deslypere, M Giri, A Vermeulen |
The Journal of Steroid Biochemistry and Molecular Biology | 1990 |
|
Luteinizing hormone pulsatility in men with damage to the germinal epithelium
JA Talbot, SM Shalet, A Tsatsoulis, M Grabinski, WR Robertson |
International Journal of Andrology | 1990 |
|
Endocrine Function and Aging
HJ Armbrecht, RM Coe, N Wongsurawat |
1990 | |
|
Pathophysiological features of the pulsatile secretion of biologically active luteinizing hormone in man
JD Veldhuis, RJ Urban, IZ Beitins, RM Blizzard, ML Johnson, ML Dufau |
Journal of Steroid Biochemistry | 1989 |
|
Hypogonadism Following Long-term Treatment with Diethylstilbestrol
J Wortsman, A Hamidinia, SJ Winters |
The American Journal of the Medical Sciences | 1989 |
|
Attenuated release of biologically active luteinizing hormone in healthy aging men
RJ Urban, JD Veldhuis, RM Blizzard, ML Dufau |
Journal of Clinical Investigation | 1988 |
|
BIOACTIVE LH IN WOMEN WITH POLYCYSTIC OVARIES AND THE EFFECT OF GONADOTROPHIN SUPPRESSION
K Mavroudis, A Evans, H Mamtora, DC Anderson, WR Robertson |
Clinical Endocrinology | 1988 |
|
Pathophysiologic Features of Episodic Gonadotropin Secretion in Man
JD Veldhuis |
The American Journal of the Medical Sciences | 1987 |
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