In this study, carried out in the rat and hamster, the receptor-dependent low density lipoprotein (LDL) transport process in each organ was characterized in terms of its maximal uptake rate (Jm) and Michaelis constant (Km), while the rate of receptor-independent uptake was defined in terms of its proportionality constant (P). The highest Jm values of 50-126 micrograms/h per g were found in the liver and endocrine glands in both species and receptor-dependent uptake also was detected in other organs like spleen, kidney, and intestine. The Km values were essentially the same in all of the organs and equaled approximately 90 mg/dl in both species. The receptor-independent uptake constants also were similar in the two species and were highest in the spleen, liver, and intestine. From these values for Jm, Km, and P, it was possible to construct theoretical curves that predict the plasma LDL-cholesterol concentration and fractional catabolic rate given any alteration in LDL-cholesterol production or the magnitude of receptor-dependent LDL transport in any organ of the rat or hamster.
D K Spady, J B Meddings, J M Dietschy
Title and authors | Publication | Year |
---|---|---|
Lipoproteins as Carriers of Pharmacological Agents
J Michael |
2017 | |
Mode-of-action evaluation for the effect of trans fatty acids on low-density lipoprotein cholesterol
JF Reichard, LT Haber |
Food and Chemical Toxicology | 2016 |
Meta-regression analysis of the effect of trans fatty acids on low-density lipoprotein cholesterol
BC Allen, MJ Vincent, DA Liska, LT Haber |
Food and Chemical Toxicology | 2016 |
The effects of Zanthoxylum bungeanum extract on lipid metabolism induced by sterols
T Wu, L Zhong, Z Hong, Y Li, X Liu, L Pan, H Xin, Y Zhu |
Journal of Pharmacological Sciences | 2014 |
Cholesterol accumulation caused by low density lipoprotein receptor deficiency or a cholesterol-rich diet results in ectopic bone formation during experimental osteoarthritis
W Munter, AB Blom, MM Helsen, B Walgreen, PM van der Kraan, LA Joosten, WB van Berg, PL van Lent |
Arthritis Research & Therapy | 2013 |
Advances in Food and Nutrition Research
N Siriwardhana, NS Kalupahana, N Moustaid-Moussa |
Marine Medicinal Foods - Implications and Applications - Animals and Microbes | 2012 |
A whole-body mathematical model of cholesterol metabolism and its age-associated dysregulation
MT Auley, DJ Wilkinson, JJ Jones, TB Kirkwood |
BMC Systems Biology | 2012 |
Effect of phospholipid n-3 polyunsaturated fatty acids on rat lipid metabolism
R Hosomi, K Fukunaga, H Arai, S Kanda, T Nishiyama, T Kanada, M Yoshida |
European Journal of Lipid Science and Technology | 2010 |
Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the golden Syrian hamster
KT Burke, PL Colvin, L Myatt, GA Graf, F Schroeder, LA Woollett |
Journal of lipid research | 2009 |
Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
KT Burke, PL Colvin, L Myatt, GA Graf, F Schroeder, LA Woollett |
Journal of lipid research | 2009 |
Cyclodextrin overcomes the transport defect in nearly every organ of NPC1 mice leading to excretion of sequestered cholesterol as bile acid
B Liu, CM Ramirez, AM Miller, JJ Repa, SD Turley, JM Dietschy |
Journal of lipid research | 2009 |
Lupin protein influences the expression of hepatic genes involved in fatty acid synthesis and triacylglycerol hydrolysis of adult rats
A Bettzieche, C Brandsch, K Weiße, F Hirche, K Eder, GI Stangl |
British Journal of Nutrition | 2007 |
Labeling of low-density lipoproteins using the 18F-labeled thiol-reactive reagent N-[6-(4-[18F]fluorobenzylidene)aminooxyhexyl]maleimide
M Berndt, J Pietzsch, F Wuest |
Nuclear Medicine and Biology | 2007 |
Receptor-mediated and bulk-phase endocytosis cause macrophage and cholesterol accumulation in Niemann-Pick C disease
B Liu, C Xie, JA Richardson, SD Turley, JM Dietschy |
Journal of lipid research | 2007 |
The effects of t10,c12 CLA isomer compared with c9,t11 CLA isomer on lipid metabolism and body composition in hamsters
V Bissonauth, Y Chouinard, J Marin, N Leblanc, D Richard, H Jacques |
The Journal of Nutritional Biochemistry | 2006 |
Preventing dyslipidemia by Chlorella pyrenoidosa in rats and hamsters after chronic high fat diet treatment
JY Cherng, MF Shih |
Life Sciences | 2005 |
The Lowering of Plasma Lipids following a Weight Reduction Program Is Related to Increased Expression of the LDL Receptor and Lipoprotein Lipase
M Patalay, IE Lofgren, HC Freake, SI Koo, ML Fernandez |
The Journal of nutrition | 2005 |
A High-Cholesterol, n–3 Polyunsaturated Fatty Acid Diet Causes Different Responses in Rats and Hamsters
MH Lin, SC Lu, PC Huang, YC Liu, SY Liu |
Annals of Nutrition and Metabolism | 2005 |
Hydroxycinnamic acids do not prevent aortic atherosclerosis in hypercholesterolemic golden Syrian hamsters
C Auger, N Laurent, C Laurent, P Besançon, B Caporiccio, PL Teissédre, JM Rouanet |
Life Sciences | 2004 |
Use of111In-L-LDL radiotracers to detect human pancreatic and mice melanoma tumors
P Urizzi, JP Souchard, G Ratovo, Y Coulais, F Nepveu, E Hollande |
Applied Organometallic Chemistry | 2003 |
Effect of Plasma Lipoproteins on Natural Killer Cell Activity in the Elderly Population
T Yasumasu, K Takahara, T Sadayasu, H Date, K Isozumi, R Kouzuma, Y Nakashima |
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences | 2003 |
Scavenger receptor class BI and selective cholesteryl ester uptake: partners in the regulation of steroidogenesis
S Azhar, E Reaven |
Molecular and Cellular Endocrinology | 2002 |
Fatty acids differentially regulate hepatic cholesteryl ester formation and incorporation into lipoproteins in the liver of the mouse
C Xie, LA Woollett, SD Turley, JM Dietschy |
Journal of lipid research | 2002 |
Red Wine Phenolic Compounds Reduce Plasma Lipids and Apolipoprotein B and Prevent Early Aortic Atherosclerosis in Hypercholesterolemic Golden Syrian Hamsters (Mesocricetus auratus)
C Auger, B Caporiccio, N Landrault, PL Teissedre, C Laurent, G Cros, P Besançon, JM Rouanet |
The Journal of nutrition | 2002 |
Effects of lovastatin and pravastatin on ubiquinone and 4-hydroxynonenal tissue levels in the hypercholesterolemic hamster
G Rousseau, M Veronneau, C Desrosiers, F Varin |
Current Therapeutic Research | 2000 |
Centripetal cholesterol flow from the extrahepatic organs through the liver is normal in mice with mutated Niemann-Pick type C protein (NPC1)
C Xie, SD Turley, JM Dietschy |
Journal of lipid research | 2000 |
Intracellular mechanisms regulating apoB-containing lipoprotein assembly and secretion in primary hamster hepatocytes
C Taghibiglou, D Rudy, SC Iderstine, A Aiton, D Cavallo, R Cheung, K Adeli |
Journal of lipid research | 2000 |
Dietary regulation of hepatic enzymes in taurine biosynthesis in rats
T Ide |
The Journal of Nutritional Biochemistry | 1998 |
Dietary Fatty Acids and the Regulation of Plasma Low Density Lipoprotein Cholesterol Concentrations
JM Dietschy |
The Journal of nutrition | 1998 |
Kinetic characteristics and regulation of HDL cholesteryl ester and apolipoprotein transport in the apoA-I-/- mouse
DK Spady, LA Woollett, RS Meidell, HH Hobbs |
Journal of lipid research | 1998 |
Kinetic parameters for high density lipoprotein apoprotein AI and cholesteryl ester transport in the hamster
LA Woollett, DK Spady |
Journal of Clinical Investigation | 1997 |
Identification of a metabolic difference accounting for the hyper- and hyporesponder phenotypes of cynomolgus monkey
SD Turley, DK Spady, JM Dietschy |
Journal of lipid research | 1997 |
Effects of dietary fatty acid composition on plasma cholesterol
P Khosla, K Sundram |
Progress in Lipid Research | 1996 |
Centripetal cholesterol flux from extrahepatic organs to the liver is independent of the concentration of high density lipoprotein-cholesterol in plasma
Y Osono, LA Woollett, KR Marotti, GW Melchior, JM Dietschy |
Proceedings of the National Academy of Sciences | 1996 |
The hypolipidemic action of the ACAT inhibitor HL-004 in hamsters fed normal chow
S Murakami, Y Ohta, Y Asami, I Yamagishi, Y Toda, M Sato, K Tomisawa |
General Pharmacology: The Vascular System | 1996 |
Cholesterol lowering action of HOE 402 in the normolipidemic and hypercholesterolemic Golden Syrian hamster
A Hoffmann, M Schmalz, M Leineweber |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1996 |
Effect of hypocholesterolemic doses of 17 alpha-ethinyl estradiol on cholesterol balance in liver and extrahepatic tissues
M Bertolotti, DK Spady |
Journal of lipid research | 1996 |
Role of the low density lipoprotein receptor in the flux of cholesterol through the plasma and across the tissues of the mouse
Y Osono, LA Woollett, J Herz, JM Dietschy |
Journal of Clinical Investigation | 1995 |
Adenovirus-mediated transfer of a gene encoding cholesterol 7 alpha-hydroxylase into hamsters increases hepatic enzyme activity and reduces plasma total and low density lipoprotein cholesterol
DK Spady, JA Cuthbert, MN Willard, RS Meidell |
Journal of Clinical Investigation | 1995 |
Low density lipoprotein binding to monolayer cultures of hepatocytes isolated from hamsters fed different dietary fatty acids
V Sessions |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1995 |
Measurement of the absolute number of functioning low-density lipoprotein receptors in vivo using a monoclonal antibody
C Fitzsimmons, R Bush, D Hele, C Godliman, E Gherardi, DE Bowyer |
Biochemical Journal | 1995 |
Apolipoprotein E competitively inhibits receptor-dependent low density lipoprotein uptake by the liver but has no effect on cholesterol absorption or synthesis in the mouse
LA Woollett, Y Osono, J Herz, JM Dietschy |
Proceedings of the National Academy of Sciences | 1995 |
Purification and characterization of a cytochrome P450 isozyme catalyzing lanosterol 14α-demethylation (P45014DM) in hamster liver
Y Sekigawa, M Fukuhara, Y Sonoda, Y Sato |
Lipids | 1995 |
Plasma lipoproteins as targeting carriers to tumour tissues after administraion of a lipophilic agent to mice
T Tokui, C Kuroiwa, S Muramatsu, Y Tokui, K Sasagawa, T Ikeda, T Komai |
Biopharmaceutics & Drug Disposition | 1995 |
Regulation of hepatic cholesterol metabolism in the rat in vivo: Effect of a synthetic fat-free diet on sterol synthesis and low-density lipoprotein transport
M Bertolotti |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1995 |
Biochemical Changes and Morphological Alterations of Liver and Kidney in Hamsters after Administration of the HMG-Coenzyme A Reductase Inhibitor, Simvastatin: Prevention and Reversibility by Mevalonate
P Oms, N Assie, F Bruniquel, AD Degryse, G Haverbeke, A Delhon |
Pharmacology & Toxicology | 1995 |
Mechanisms of LDL-cholesterol lowering action of psyllium hydrophillic mucilloid in the hamster
SD Turley, JM Dietschy |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1995 |
Regulation of Hepatic 7α-Hydroxylase Expression and Response to Dietary Cholesterol in the Rat and Hamster
JD Horton, JA Cuthbert, DK Spady |
The Journal of biological chemistry | 1995 |
Role of liver in the synthesis of cholesterol and the clearance of low density lipoproteins in the cynomolgus monkey
SD Turley, DK Spady, JM Dietschy |
Journal of lipid research | 1995 |
Regulatory effects of n-3 polyunsaturated fatty acids on hepatic LDL uptake in the hamster and rat
DK Spady, JD Horton, JA Cuthbert |
Journal of lipid research | 1995 |
Regulation of hepatic 7 alpha-hydroxylase expression by dietary psyllium in the hamster
JD Horton, JA Cuthbert, DK Spady |
Journal of Clinical Investigation | 1994 |
Reevaluation and application of the dual-isotope plasma ratio method for the measurement of intestinal cholesterol absorption in the hamster
SD Turley, MW Herndon, JM Dietschy |
Journal of lipid research | 1994 |
Trans-9-octadecenoic acid is biologically neutral and does not regulate the low density lipoprotein receptor as the cis isomer does in the hamster
LA Woollett, CM Daumerie, JM Dietschy |
Journal of lipid research | 1994 |
Dietary fatty acids regulate hepatic low density lipoprotein (LDL) transport by altering LDL receptor protein and mRNA levels
JD Horton, JA Cuthbert, DK Spady |
Journal of Clinical Investigation | 1993 |
Animal models of human lipid metabolism
KE Suckling, B Jackson |
Progress in Lipid Research | 1993 |
The Interaction of Dietary Cholesterol and Specific Fatty Acids in the Regulation of LDL Receptor Activity and Plasma LDL-Cholesterol Concentrations
JM Dietschy, LA Woollett, DK Spady |
Annals of the New York Academy of Sciences | 1993 |
Hypocholesterolaemic effect of beta beta'-methyl-substituted hexadecanedioic acid (MEDICA 16) in the male hamster
N Mayorek, J Bar-Tana |
Biochemical Journal | 1993 |
Role of liver in the maintenance of cholesterol and low density lipoprotein homeostasis in different animal species, including humans
JM Dietschy, SD Turley, DK Spady |
Journal of lipid research | 1993 |
Regulatory effects of individual n-6 and n-3 polyunsaturated fatty acids on LDL transport in the rat
DK Spady |
Journal of lipid research | 1993 |
Plasma lipoproteins in the golden Syrian hamster (Mesocricetus auratus): heterogeneity of apoB- and apoA-I-containing particles
S Goulinet, MJ Chapman |
Journal of lipid research | 1993 |
Regulatory effects of the saturated fatty acids 6:0 through 18:0 on hepatic low density lipoprotein receptor activity in the hamster
LA Woollett, DK Spady, JM Dietschy |
Journal of Clinical Investigation | 1992 |
Cytoplasmic sequence required for basolateral targeting of LDL receptor in livers of transgenic mice
M Yokode, RK Pathak, RE Hammer, MS Brown, JL Goldstein, RG Anderson |
The Journal of Cell Biology | 1992 |
Fatty acids regulate hepatic low density lipoprotein receptor activity through redistribution of intracellular cholesterol pools
CM Daumerie, LA Woollett, JM Dietschy |
Proceedings of the National Academy of Sciences | 1992 |
Cholesterol metabolism in ExHC (exogenous hypercholesterolemic) rats
K Imaizumi, A Nagatomi, M Sato, A Tominaga, M Sugano |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1992 |
Effect of copper deficiency on the plasma clearance of native and acetylated human low density lipoproteins
SI Koo, CC Lee, WL Stone, RL Scott |
The Journal of Nutritional Biochemistry | 1992 |
Expression of 7 alpha-hydroxylase in non-hepatic cells results in liver phenotypic resistance of the low density lipoprotein receptor to cholesterol repression
S Dueland, JD Trawick, MS Nenseter, AA MacPhee, RA Davis |
The Journal of biological chemistry | 1992 |
Regulation of hepatic sterol metabolism in the rat. Parallel regulation of activity and mRNA for 7 alpha-hydroxylase but not 3-hydroxy-3-methylglutaryl-coenzyme A reductase or low density lipoprotein receptor
DK Spady, JA Cuthbert |
The Journal of biological chemistry | 1992 |
Use of an anti-low density lipoprotein receptor antibody to quantify the role of the LDL receptor in the removal of chylomicron remnants in the mouse in vivo
SY Choi, LG Fong, MJ Kirven, AD Cooper |
Journal of Clinical Investigation | 1991 |
The association of hepatic apoprotein and lipid metabolism in hamsters and rats
GL Liu, LM Fan, RN Redinger |
Comparative Biochemistry and Physiology Part A: Physiology | 1991 |
Native and modified lipoproteins as drug delivery systems
MK Bijsterbosch, TJ van Berkel |
Advanced Drug Delivery Reviews | 1990 |
Low density lipoprotein receptor-binding activity in human tissues: quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo
MJ Rudling, E Reihnér, K Einarsson, S Ewerth, B Angelin |
Proceedings of the National Academy of Sciences | 1990 |
Tissue accumulation of lipoprotein associated toxaphene in normo- and hypolipidemic mice
A Mohammed, A Eklund, AM Östlund-Lindqvist, P Slanina |
Archives of Toxicology | 1990 |
Analysis of low density lipoprotein apheresis: post-treatment rebound using mathematical models
A Werynski, PS Malchesky, H Nomura, Y Nose |
Computer Methods and Programs in Biomedicine | 1990 |
Lipoprotein interactions with T cells: an update
KN Traill, LA Huber, G Wick, G Jürgens |
Immunology Today | 1990 |
LDL Cholesterol: Its Regulation and Manipulation
JM Dietschy |
Hospital Practice | 1990 |
Interaction of dietary saturated and polyunsaturated triglycerides in regulating the processes that determine plasma low density lipoprotein concentrations in the rat
DK Spady, LA Woollett |
Journal of lipid research | 1990 |
Tracer kinetic studies of the low density lipoprotein metabolism in the fetal rat: an example for estimation of flux rates in the nonsteady state
D Plonné, B Schlag, L Winkler, R Dargel |
Journal of lipid research | 1990 |
Metabolic factors underlying high serum triglycerides in the normal hamster
JA Ontko, Q Cheng, M Yamamoto |
Journal of lipid research | 1990 |
Tissue accumulation of lipoprotein associated toxaphene in normo- and hypolipidemic mice
A Mohammed, A Eklund, , P Slanina |
Archives of Toxicology | 1990 |
Mechanisms by which saturated triacylglycerols elevate the plasma low density lipoprotein-cholesterol concentration in hamsters. Differential effects of fatty acid chain length
LA Woollett, DK Spady, JM Dietschy |
Journal of Clinical Investigation | 1989 |
Dietary fish oil stimulates hepatic low density lipoprotein transport in the rat
MA Ventura, LA Woollett, DK Spady |
Journal of Clinical Investigation | 1989 |
The low density lipoprotein receptor: Structure, function and pharmacological modulation
AL Catapano |
Pharmacology & Therapeutics | 1989 |
Lipoprotein Subfractions Omega-3 Fatty Acids
HU Klör |
1989 | |
Interaction of aging and dietary fat in the regulation of low density lipoprotein transport in the hamster
DK Spady, JM Dietschy |
Journal of lipid research | 1989 |
A rapid method to assess the hydrophobicity of the intestinal microvillus membrane in vivo
JB Meddings, JM Dietschy |
Journal of lipid research | 1989 |
Receptor-mediated transport of peptide hormones and its importance in the overall hormone disposition in the body
Y Sugiyama, M Hanano |
Pharmaceutical Research | 1989 |
Interaction of dietary cholesterol and triglycerides in the regulation of hepatic low density lipoprotein transport in the hamster
DK Spady, JM Dietschy |
Journal of Clinical Investigation | 1988 |
Hepatocytic lipoprotein receptors and intracellular lipoprotein catabolism
RJ Havel, RL Hamilton |
Hepatology | 1988 |
Regulation of function and level of expression of the LDL-receptor gene
S Humphries, D Bowyer |
Atherosclerosis | 1988 |
Kinetic analysis of in vivo receptor-dependent binding of human epidermal growth factor by rat tissues
DC Kim, Y Sugiyama, H Satoh, T Iga, M Hanano, T Fuwa |
Journal of Pharmaceutical Sciences | 1988 |
The effects of 6-azacholest-4-en-3β-ol-7-one, an inhibitor of cholesterol 7α-hydroxylase, on cholesterol metabolism and bile acid synthesis in primary cultures of rat hepatocytes
WJ Sampson, JD Houghton, P Bowers, RA Suffolk, KM Botham, CJ Suckling, KE Suckling |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1988 |
Characterization of apoB, E receptor function in the luteinized ovary
S Azhar, A Cooper, L Tsai, W Maffe, E Reaven |
Journal of lipid research | 1988 |
Mechanisms of primary hypercholesterolemia in humans
GL Vega, SM Grundy |
American Heart Journal | 1987 |
Kinetic characteristics and mechanisms of regulation of receptor-dependent and receptor-independent LDL transport in the liver of different animal species and humans
JB Meddings, DK Spady, JM Dietschy |
American Heart Journal | 1987 |
Isolation and characterization of four heparin-binding cyanogen bromide peptides of human plasma apolipoprotein B
N Hirose, DT Blankenship, KM A., RL Jackson, AD Cardin |
Biochemistry | 1987 |
Regulation of Plasma Low Density Lipoprotein Levels Biopharmacological Regulation of Protein Phosphorylation Calcium-Activated Neutral Protease Microbial Iron Transport Pharmacokinetic Drug Interactions
WR Bartle, V Braun, JM Dietschy, Y Emori, M Hagiwara, H Hidaka, S Imajoh, H Kawasaki, JB Meddings, S Ohno, K Suzuki, SE Walker, G Winkelmann, NE Winslade |
1987 | |
Provision of cholesterol to lymphocytes by high density and low density lipoproteins. Requirement for low density lipoprotein receptors
JA Cuthbert, PE Lipsky |
The Journal of biological chemistry | 1987 |
Regulation of plasma levels of low-density lipoprotein cholesterol: interpretation of data on low-density lipoprotein turnover in man
JB Meddings, JM Dietschy |
Circulation | 1986 |
Role of receptor-independent low density lipoprotein transport in the maintenance of tissue cholesterol balance in the normal and WHHL rabbit
DK Spady, M Huettinger, DW Bilheimer, JM Dietschy |
Journal of lipid research | 1986 |