Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Lipoproteins containing apoprotein B are a major regulator of neutrophil responses to monosodium urate crystals.
R Terkeltaub, … , A J Tenner, M H Ginsberg
R Terkeltaub, … , A J Tenner, M H Ginsberg
Published June 1, 1984
Citation Information: J Clin Invest. 1984;73(6):1719-1730. https://doi.org/10.1172/JCI111380.
View: Text | PDF
Research Article

Lipoproteins containing apoprotein B are a major regulator of neutrophil responses to monosodium urate crystals.

  • Text
  • PDF
Abstract

The inflammatory response to intraarticular urate crystals is known to be variable in gouty arthritis. One source of variability may be the modulation of cellular responses by crystal-bound proteins. We have identified three apolipoproteins among the polypeptides bound to urate crystals exposed to plasma. Identification was first based on their coelectrophoresis with polypeptides from isolated lipoproteins and diminution in the protein coat of crystals exposed to lipoprotein-depleted plasma. The apoproteins were immunochemically identified by the Western blotting technique as apoprotein A-I, apoprotein B (apo B), and apoprotein E. Because neutrophils play a central role in acute gout, we investigated the potential effects of lipoproteins on neutrophil-urate crystal interactions. Plasma profoundly inhibited urate crystal-induced neutrophil luminol-dependent chemiluminescence (CL). Lipoprotein depletion by KBr density gradient centrifugation completely abrogated the inhibitory effect of plasma on urate-induced CL. The inhibitory activity of lipoprotein-depleted plasma was restored by adding back the d less than or equal to 1.25 g/cm3 lipoprotein fraction. Plasma also inhibited urate crystal-induced neutrophil superoxide generation and cytolysis (lactic dehydrogenase loss). This inhibition was significantly diminished by lipoprotein depletion, indicating that the lipoprotein effect was not limited to CL. Lipoprotein-depleted plasma reconstituted with very low, intermediate, and low density lipoproteins (LDL) inhibited crystal-induced CL. High density lipoprotein reconstitution was without effect. Immunodepletion from plasma of all apo B lipoproteins by agarose-bound apo B-specific antibody also removed all inhibitory activity for urate-induced CL. Thus, apo B lipoproteins were shown to be the inhibitory species in plasma. Binding of apo B lipoproteins to urate crystals and inhibition of CL was also seen in the absence of other plasma proteins. In addition, the binding of whole lipoprotein particles to the crystals was verified by detection of crystal-associated cholesterol in addition to the apoprotein. The effects of LDL on urate crystal-induced CL were stimulus specific. Coincubation of urate crystals and neutrophils in the presence of 10 micrograms/ml LDL resulted in 83% inhibition. In contrast, CL responses to a chemotactic hexapeptide, opsonized zymosan, and Staphylococcus aureus were not inhibited by LDL. The effects of depletion of apo B lipoproteins on plasma suppression of urate crystal-induced CL appeared to be unique. Plasma or sera depleted of other urate crystal-binding proteins including fibrinogen, fibronectin, C1q, and IgG retained virtually all their CL inhibitory activity. Lipoproteins containing apo B are thus a major regulator of neutrophil responses to urate crystals. These lipoproteins are present in variable concentration in synovial fluid and may exert an important influence on the course of gout.

Authors

R Terkeltaub, L K Curtiss, A J Tenner, M H Ginsberg

×

Total citations by year

Year: 2023 2022 2020 2019 2015 2014 2013 2012 2011 2009 2008 2006 2005 2003 2002 2000 1999 1997 1996 1995 1993 1992 1991 1990 1989 1988 1987 1986 1985 Total
Citations: 1 1 2 1 1 3 1 1 3 3 1 3 2 1 1 1 1 2 1 4 4 2 6 2 2 3 1 5 3 62
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (62)

Title and authors Publication Year
The Independent Value of Neutrophil to Lymphocyte Ratio in Gouty Arthritis: A Narrative Review.
Chen Y, Liu J, Li Y, Cong C, Hu Y, Zhang X, Han Q
Journal of inflammation research 2023
Natural antibodies and CRP drive anaphylatoxin production by urate crystals.
Wessig AK, Hoffmeister L, Klingberg A, Alberts A, Pich A, Brand K, Witte T, Neumann K
Scientific Reports 2022
C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1
A Alberts, A Klingberg, AK Wessig, C Combes, T Witte, K Brand, A Pich, K Neumann
Scientific Reports 2020
Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures
A Alberts, A Klingberg, L Hoffmeister, AK Wessig, K Brand, A Pich, K Neumann
Frontiers in immunology 2020
Adsorption of Proteins on m-CPPD and Urate Crystals Inhibits Crystal-induced Cell Responses: Study on Albumin-crystal Interaction
Renaudin, Sarda, CG, Séverac, Léger, Charvillat, Rey, Lioté, Camadro, Ea, Combes
Journal of Functional Biomaterials 2019
Inflammation: a possible mechanism for a causative role of hyperuricemia/gout in cardiovascular disease
F Perez-Ruiz, MA Becker
Current Medical Research and Opinion 2015
The relationship of apolipoprotein B and very low density lipoprotein triglyceride with hyperuricemia and gout
H Rasheed, A Hsu, N Dalbeth, LK Stamp, S McCormick, TR Merriman
Arthritis Research & Therapy 2014
Mechanism of Action of Colchicine in the Treatment of Gout
N Dalbeth, TJ Lauterio, HR Wolfe
Clinical Therapeutics 2014
Increased level of MSU crystal-bound protein apolipoprotein A-I in acute gouty arthritis
SL Chiang, TT Ou, YJ Wu, HP Tu, CY Lu, CM Huang, TM Kuo, TN Wang, CH Chou, YC Ko
Scandinavian Journal of Rheumatology 2014
High-density lipoproteins inhibit urate crystal-induced inflammation in mice
A Scanu, R Luisetto, F Oliviero, L Gruaz, P Sfriso, D Burger, L Punzi
Annals of the rheumatic diseases 2013
Tophus Biology and Pathogenesis of Monosodium Urate Crystal–Induced Inflammation
R Liu-Bryan, R Terkeltaub
Gout &amp Other Crystal Arthropathies 2012
The role of the NLRP3 inflammasome in gout
SR Kingsbury, PG Conaghan, MF McDermott
Journal of inflammation research 2011
Uric acid is a danger signal of increasing risk for osteoarthritis through inflammasome activation
AE Denoble, KM Huffman, TV Stabler, SJ Kelly, MS Hershfield, GE McDaniel, RE Coleman, VB Kraus
Proceedings of the National Academy of Sciences 2011
A comparative study of serum and synovial fluid lipoprotein levels in patients with various arthritides
F Oliviero, AL Nigro, D Bernardi, S Giunco, G Baldo, A Scanu, P Sfriso, R Ramonda, M Plebani, L Punzi
Clinica Chimica Acta 2011
Crystal-induced neutrophil activation
O Popa-Nita, PH Naccache
Immunology and Cell Biology 2009
Innate inflammation and resolution in acute gout
WJ Martin, JL Harper
Immunology and Cell Biology 2009
Resident macrophages initiating and driving inflammation in a monosodium urate monohydrate crystal-induced murine peritoneal model of acute gout
WJ Martin, M Walton, J Harper
Arthritis & Rheumatism 2009
Colchicine: Its mechanism of action and efficacy in crystal-induced inflammation
G Nuki
Current Rheumatology Reports 2008
Evil humors take their toll as innate immunity makes gouty joints TREM-ble
R Liu-Bryan, R Terkeltaub
Arthritis & Rheumatism 2006
Gout: Update on Some Pathogenic and Clinical Aspects
F Lioté, HK Ea
Rheumatic Disease Clinics of North America 2006
Goutte
D Kuntz, F Lioté
EMC - Appareil locomoteur 2006
Innate immunity conferred by toll-like receptors 2 and 4 and myeloid differentiation factor 88 expression is pivotal to monosodium urate monohydrate crystal-induced inflammation
R Liu-Bryan, P Scott, A Sydlaske, DM Rose, R Terkeltaub
Arthritis & Rheumatism 2005
Gout in Solid Organ Transplantation: A Challenging Clinical Problem
L Stamp, M Searle, J ODonnell, P Chapman
Drugs 2005
Gota
D Kuntz, F Lioté
EMC - Aparato Locomotor 2003
Safe disposal of inflammatory monosodium urate monohydrate crystals by differentiated macrophages
RC Landis, DR Yagnik, O Florey, P Philippidis, V Emons, JC Mason, DO Haskard
Arthritis & Rheumatism 2002
Neutrophil activation by plasma opsonized polymeric microspheres: inhibitory effect of Pluronic F127
JK Jackson, CM Springate, WL Hunter, HM Burt
Biomaterials 2000
Silica Binds Serum Proteins Resulting in a Shift of the Dose-Response for Silica-Induced Chemokine Expression in an Alveolar Type II Cell Line
EG Barrett, C Johnston, G Oberdörster, JN Finkelstein
Toxicology and Applied Pharmacology 1999
Effects of Human Low-Density Lipoproteins on Superoxide Production by Formyl-Methionyl-Leucyl-Phenylalanine Activated Polymorphonuclear Leukocytes
C Bonneau, R Couderc, M Tissot, A Athias, M Roch-Arveiller, JP Giroud
Clinical Chemistry and Laboratory Medicine 1997
Activation of human neutrophils by calcium carbonate polymorphs
H M Burt, J K Jackson, D R Taylor, R S Crowther
Digestive Diseases and Sciences 1997
The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils
CI Winternitz, JK Jackson, HM Burt
Rheumatology International 1996
Triglyceride-rich lipoproteins improve survival when given after endotoxin in rats
TE Read, C Grunfeld, Z Kumwenda, MD Calhoun, JP Kane, KR Feingold, JH Rapp
Surgery 1995
Triglyceride-rich lipoproteins prevent septic death in rats
TE Read, C Grunfeld, ZL Kumwenda, MC Calhoun, JP Kane, KR Feingold, JH Rapp
Journal of Experimental Medicine 1995
Role for circulating lipoproteins in protection from endotoxin toxicity
KR Feingold, JL Funk, AH Moser, JK Shigenaga, JH Rapp, C Grunfeld
Infection and immunity 1995
Evidence for a causal relationship between the structure, size, and load of calcium pyrophosphate dihydrate crystals, and attacks of pseudogout
A Swan, B Heywood, B Chapman, H Seward, P Dieppe
Annals of the rheumatic diseases 1995
Chylomicrons alter the fate of endotoxin, decreasing tumor necrosis factor release and preventing death
HW Harris, C Grunfeld, KR Feingold, TE Read, JP Kane, AL Jones, EB Eichbaum, GF Bland, JH Rapp
Journal of Clinical Investigation 1993
Apolipoproteins A-I and B and cholesterol in synovial fluid of patients with rheumatoid arthritis
L Ananth, PE Prete, ML Kashyap
Metabolism 1993
Enhancement of crystal induced neutrophil responses by opsonisation of calcium pyrophosphate dihydrate crystals
HM Burt, JK Jackson
Annals of the rheumatic diseases 1993
Effect of endotoxin on cholesterol biosynthesis and distribution in serum lipoproteins in Syrian hamsters
KR Feingold, I Hardardottir, R Memon, EJ Krul, AH Moser, JM Taylor, C Grunfeld
Journal of lipid research 1993
Morphological Evidence for Biological Control of Urate Crystal Formation in Vivo and in Vitro
NW McGill, A Hayes, PA Dieppe
Scandinavian Journal of Rheumatology 1992
Metabolic Disturbances and Wasting in the Acquired Immunodeficiency Syndrome
JS Flier, LH Underhill, C Grunfeld, KR Feingold
New England Journal of Medicine 1992
Apolipoprotein (apo) E inhibits the capacity of monosodium urate crystals to stimulate neutrophils. Characterization of intraarticular apo E and demonstration of apo E binding to urate crystals in vivo
RA Terkeltaub, CA Dyer, J Martin, LK Curtiss
Journal of Clinical Investigation 1991
Monocyte-derived neutrophil chemotactic factor/interleukin-8 is a potential mediator of crystal-induced inflammation
R Terkeltaub, C Zachariae, D Santoro, J Martin, P Peveri, K Matsushima
Arthritis & Rheumatism 1991
Tophus-derived monosodium urate monohydrate crystals are biologically much more active than synthetic counterpart
A Stanković, P Front, A Barbara, DR Mitrović
Rheumatology International 1991
Crystal-induced neutrophil activation. I. Initiation and modulation of calcium mobilization and superoxide production by microcrystals
PH Naccache, M Grimard, CJ Roberge, C Gilbert, PE Poubelle, A Lussier, RD Médicis
Arthritis & Rheumatism 1991
Functional differences in human neutrophils isolated pre- and post-prandially
DJ Uhlinger, DN Burnham, RE Mullins, JR Kalmar, CW Cutler, RR Arnold, JD Lambeth, AH Merrill
FEBS Letters 1991
Urate Deposition in Man and its Clinical Consequences
U Gresser, N Zöllner
1991
The effects of γ-interferon on human peripheral blood monocyte/macrophage-mediated bone particle degradation
R Terkeltaub, GS Firestein, RS Kornbluth, J Martin, D Curran, S Manolagas
Bone and Mineral 1990
Hyperurikämie, Gicht und andere Störungen des Purinhaushalts
N Zöllner
1990
Lipid Modulators of Cell Function
AH Merrill
Nutrition Reviews 1989
Lipoproteins alter the catalytic behavior of the platelet-activating factor acetylhydrolase in human plasma
DM Stafforini, ME Carter, GA Zimmerman, TM McIntyre, SM Prescott
Proceedings of the National Academy of Sciences 1989
Serum and plasma inhibit neutrophil stimulation by hydroxyapatite crystals. evidence that serum αl2-hs glycoprotein is a potent and specific crystal-bound inhibitor
RA Terkeltaub, DA Santoro, G Mandel, N Mandel
Arthritis & Rheumatism 1988
Crystal-Associated Arthropathies
AJ Reginato, HR Schumacher
Clinics in Geriatric Medicine 1988
The Inflammatory Reaction to Crystals
RA Terkeltaub, MH Ginsberg
Rheumatic diseases clinics of North America 1988
Comparison of phenotype expression by mononuclear phagocytes within subcutaneous gouty tophi and rheumatoid nodules
DG Palmer, N Hogg, I Denholm, CA Allen, J Highton, PA Hessian
Rheumatology International 1987
Low density lipoprotein inhibits the physical interaction of phlogistic crystals and inflammatory cells
R Terkeltaub, D Smeltzer, LK Curtiss, MH Ginsberg
Arthritis & Rheumatism 1986
Preliminary evidence for the presence of an inhibitor on the surface of natural monosodium urate crystals
TP Gordon, PJ Roberts-Thomson
Arthritis & Rheumatism 1986
Products of polymorphonuclear cell injury inhibit IgG enhancement of monosodium urate-induced superoxide production
MS Rosen, DG Baker, HR Schumacher, PV Cherian
Arthritis & Rheumatism 1986
Lack of correlation between in vitro and in vivo effects of low density lipoprotein on the inflammatory activity of monosodium urate crystals
TP Gordon, P Clifton, MJ James, PJ Roberts-Thomson
Annals of the rheumatic diseases 1986
Apolipoprotein B mediates the capacity of low density lipoprotein to suppress neutrophil stimulation by particulates
R Terkeltaub, J Martin, LK Curtiss, MH Ginsberg
The Journal of biological chemistry 1986
Immunology of Rheumatic Diseases
S Gupta, N Talal
1985
Studies on the interaction of rheumatoid factor with monosodium urate crystals and case report of coexistent tophaceous gout and rheumatoid arthritis
TP Gordon, MJ Ahern, C Reid, PJ Roberts-Thomson
Annals of the rheumatic diseases 1985
Calcinosis of joints and periarticular tissues associated with vitamin D intoxication
RC Butler, PA Dieppe, AC Keat
Annals of the rheumatic diseases 1985

← Previous 1 2 3 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts