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Citations to this article

Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.
D K Podolsky, K J Isselbacher
D K Podolsky, K J Isselbacher
Published July 1, 1983
Citation Information: J Clin Invest. 1983;72(1):142-153. https://doi.org/10.1172/JCI110952.
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Research Article

Composition of human colonic mucin. Selective alteration in inflammatory bowel disease.

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Abstract

Human colonic mucin has been isolated from mucosal scrapings of fresh surgical specimens of normal controls as well as patients with Crohn's colitis and ulcerative colitis. Following sonication and ultracentrifugation, mucin fractions were separated from other soluble colonic glycoproteins by Sepharose 4B chromatography. After nuclease digestion, cesium chloride gradient centrifugation of the excluded material yielded colonic mucin with an average buoyant density of 1.52 g/ml. Subsequent chromatography of the apparently homogeneous colonic mucin on DEAE-cellulose revealed the presence of at least six distinct mucin species (mucin I-VI). Each mucin species was found to have a distinctive hexose, hexosamine, sialic acid, and sulfate content as well as blood group substance activities. Mucin from five patients with Crohn's colitis was found to represent a mixture of at least six discrete species comparable to those isolated from normal colonic specimens. However, in mucin from eight patients with ulcerative colitis there was a marked and selective reduction of one component mucin subclass, designated species IV. Normal mucin and mucin from patients with Crohn's disease contained 48 +/- 17 and 42 +/- 12 mg of species IV/g, while mucin from patients with ulcerative colitis had 5 +/- 3 mg/g solubilized glycoprotein. The selective absence of species IV was found in preparations from both sigmoid (n = 7) and ascending (n = 4) colon and could not be accounted for by an overall decrease in total mucin content. The selective reduction of species IV was also found in mucin isolated from relatively noninflamed colonic mucosa of patients with ulcerative colitis. The carbohydrate composition and blood group activities of the remaining five mucin species were similar to their normal counterparts. Based on the results to date, there appears to be an underlying selective decrease of one colonic mucin subclass in ulcerative colitis.

Authors

D K Podolsky, K J Isselbacher

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Total citations by year

Year: 2021 2020 2019 2017 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2002 2001 2000 1999 1998 1997 1996 1994 1993 1992 1991 1990 1989 1988 1987 1986 1985 1984 1983 Total
Citations: 2 2 4 1 3 2 3 1 1 4 5 7 2 2 1 3 1 7 2 6 1 3 5 6 4 4 9 10 3 13 9 3 8 2 1 140
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2010 (4)

Title and authors Publication Year
New Insights in the Role of Fatty Acids in the Pathogenesis and Resolution of Inflammatory Bowel Disease
Darla Shores, David Binion, Bruce Freeman, Paul Baker
Inflammatory Bowel Diseases 2010
Intestinal Host-Microbe Interactions under Physiological and Pathological Conditions
R Bibiloni, EJ Schiffrin
International Journal of Inflammation 2010
Identification of complement C3 as an autoantigen in inflammatory bowel disease
BA Lundgren, F Rorsman, GM Portela-Gomes, L Grimelius, KN Ekdahl, B Nilsson, O Ekwall
European Journal of Gastroenterology & Hepatology 2010
Identification of complement C3 as an autoantigen in inflammatory bowel disease:
BA Lundgren, F Rorsman, GM Portela-Gomes, L Grimelius, KN Ekdahl, B Nilsson, O Ekwall
European Journal of Gastroenterology & Hepatology 2010

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