The interaction of hemoglobin C (Hb C) with erythrocyte membranes was studied using changes in fluorescence intensity in a membrane-embedded probe. The affinity of Hb C for the membranes at pH 6.0 and pH 6.8 was compared to that of normal hemoglobin (Hb A). Steady-state and kinetic data were delivered. The affinity of Hb C for the erythrocyte membrane at pH 6.8 appeared to be about five times greater than that of Hb A. The associations of Hb C and Hb A with the membrane were reversible to about the same extent. The cytoplasmic portions of band 3 membrane proteins were suggested to be the binding sites for both hemoglobins. The membrane binding of Hb C at pH values of 6.8 to 7.0 indicates that this reaction may occur under physiological circumstances.
G H Reiss, H M Ranney, N Shaklai
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Altered membrane structure and surface potential in homozygous hemoglobin C erythrocytes
F Tokumasu, GA Nardone, GR Ostera, RM Fairhurst, SD Beaudry, E Hayakawa, JA Dvorak |
PloS one | 2009 |
Low concentration of extracellular hemoglobin affects shape of RBC in low ion strength sucrose solution
SV Rudenko |
Bioelectrochemistry | 2009 |
Heme degradation and oxidative stress in murine models for hemoglobinopathies: Thalassemia, sickle cell disease and hemoglobin C disease
E Nagababu, ME Fabry, RL Nagel, JM Rifkind |
Blood Cells, Molecules, and Diseases | 2008 |
Phase separation and crystallization of hemoglobin C in transgenic mouse and human erythrocytes
JE Canterino, O Galkin, PG Vekilov, RE Hirsch |
Biophysical Journal | 2008 |
Non-opsonising aggregates of IgG and complement in haemoglobin C erythrocytes
NJ Brittain, C Erexson, L Faucette, J Ward, H Fujioka, TE Wellems, RM Fairhurst |
British Journal of Haematology | 2006 |
Human and mouse hemoglobin association with the transgenic mouse erythrocyte membrane
Q Chen, TC Balazs, RL Nagel, RE Hirsch |
FEBS Letters | 2006 |
Hemoglobin C modulates the surface topography of Plasmodium falciparum-infected erythrocytes
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A hypothesis on the role of the electrical charge of haemoglobin in regulating the erythrocyte shape
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Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells
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Red cell osmotic fragility studies in hemoglobin C-beta thalassemia: osmotically resistant microspherocytes
DA Sears, MM Udden, MD Johnston |
Clinical and Laboratory Haematology | 2003 |
Beta7E-beta132K salt bridge and sickle haemoglobin stability and conformation
Christophe Fablet, Qiuying Chen, Véronique Baudin-Creuza, Michael C Marden, Ronald L Nagel, Josée Pagnier, Rhoda Elison Hirsch |
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HT Terra, MJ Saad, CR Carvalho, DL Vicentin, FF Costa, ST Saad |
American Journal of Hematology | 1998 |
Molecular Biology of Membrane Transport Disorders
SG Schultz, TE Andreoli, AM Brown, DM Fambrough, JF Hoffman, MJ Welsh |
1996 | |
Hemoglobin C disease in infancy and childhood
JF Olson, RE Ware, WH Schultz, TR Kinney |
The Journal of Pediatrics | 1994 |
Rate of activation and deactivation of K?Cl cotransport by changes in cell volume in hemoglobin SS, CC and AA red cells
M Canessa, JR Romero, C Lawrence, RL Nagel, ME Fabry |
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I Kirschner-Zilber, E Setter, N Shaklai |
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TE Andreoli, JF Hoffman, DD Fanestil, SG Schultz |
1987 | |
Purification and properties of human erythrocyte band 4.2. Association with the cytoplasmic domain of band 3
C Korsgren, CM Cohen |
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OS Platt, JF Falcone, SE Lux |
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C Brugnara, AS Kopin, HF Bunn, DC Tosteson |
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