Citations to this article

Production and characterization of a cytotoxic monoclonal antibody reacting with rat islet cells.
M A Crump, … , W Kortz, G S Eisenbarth
M A Crump, … , W Kortz, G S Eisenbarth
Published September 1, 1982
Citation Information: J Clin Invest. 1982;70(3):659-666. https://doi.org/10.1172/JCI110660.
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Production and characterization of a cytotoxic monoclonal antibody reacting with rat islet cells.

Abstract

We have produced a murine monoclonal antibody (F43 A1D2) that binds to the cell surface of both rat islet tumor cells (RINm clone 5F and RINm clone 14B) and normal rat islet cells. This antibody is cytotoxic in the presence of complement for RIN tumor cells as well as A, B, and D pancreatic polypeptide rat islet cells. Antibody A1D2 does not bind to rat thymus cells, pancreatic acinar cells, or fibroblasts. Antigen A1D2 (termed ICSAn-1 [islet cell surface antigen 1]) is a trypsin-sensitive glycoprotein with a molecular mass of approximately 24,000 daltons. In addition to purification of its islet cell antigen, antibody A1D2 can be used to identify and isolate living islet cells from pancreatic digests.

Authors

M A Crump, R Scearce, M Dobersen, W Kortz, G S Eisenbarth

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Year: 2013 1990 1989 1988 1987 1986 1984 1983 1982 1953 Total
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Citations to this article (15)

Title and authors Publication Year
George S. Eisenbarth: insulin and type 1 diabetes
A Pugliese, JS Skyler 		Diabetes care 2013
Heterogeneity of islet cell autoantibodies in terms of insulin release from rat islets and insulinoma cells
K Ejiri, H Taniguchi, Y Morimoto, Y Yamashiro, S Baba, S Srikanta, GS Eisenbarth 		Diabetes Research and Clinical Practice 1990
Immunological aspects of diabetes mellitus: Prospects for pharmacological modification
M Itoh 		Pharmacology & Therapeutics 1989
Reactivity of monoclonal islet cell antibodies on normal hyperplastic and neoplastic thyroid C-cells
N Neuhold, M Wimmer, OM Braun, G Horvat, K Krisch 		Cell and Tissue Research 1989
Detection of Antibodies against Wheat Germ Agglutinin Bound Glycoproteins on the Islet-cell Membrane
N Kitano, T Taminato, T Ida, M Seno, Y Seino, S Matsukura, S Kuno, H Imura 		Diabetic Medicine 1988
Human Nesidioblastosis Tissue as an Immunogen for Generation of Islet Cell Specific Monoclonal Antibodies
KD Gerbitz, B Boenke, A Paprotta, U Chemnitz, G Hübner 		Clinical Chemistry and Laboratory Medicine 1988
Monoclonal antibodies against human islets of Langerhans
, B Boenke, A Paprotta, U Chemnitz 		Fresenius' Zeitschrift f�r Analytische Chemie 1988
Pancreatic islet cell surface glycoproteins containing Gal beta 1-4GlcNAc-R identified by a cytotoxic monoclonal autoantibody
Y Uchigata, SL Spitalnik, O Tachiwaki, KF Salata, AL Notkins 		Journal of Experimental Medicine 1987
Virus Infections and Diabetes Mellitus
Y Becker 		1987
Monoclonal Antibodies against Pancreatic Islet-Cell-Surface Antigens Selected by Flow Cytofluorometry
H Vissing, G Papadopoulos, Å Lernmark 		Scandinavian Journal of Immunology 1986
A simple assay for the detection of antibodies to endocrine islet cell surface antigens
G Contreas, OD Madsen, H Vissing, Å Lernmark 		Journal of Immunological Methods 1986
A ganglioside antigen on the rat pancreatic B cell surface identified by monoclonal antibody R2D6
R Alejandro, FL Shienvold, SA Hajek, M Pierce, R Paul, DH Mintz 		Journal of Clinical Investigation 1984
Monoclonal Antibodies
DS Pisetsky 		Monoclonal Antibodies 1983
Human hybridomas secreting anti-islet autoantibodies
GS Eisenbarth, A Linnenbach, R Jackson, R Scearce, CM Croce 		Nature 1982
Clinical Chemistry
RM Archibald 		Analytical Chemistry 1953

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