The consequences of K recycling and accumulation in the renal medulla were examined by measuring the effect of elevated K concentration on ion transport by the medullary thick ascending limb of Henle's loop. Perfused and bathed in vitro, thick limbs from both mouse and rabbit displayed a graded, reversible reduction of transepithelial voltage after increasing K concentration from 5 to 10, 15, or 25 mM. The effect was reproducible whether osmolality was 328 or 445 mosmol/kg H2O, and whether K replaced Na or choline. Net chloride absorption and transepithelial voltage were reduced by almost 90% when ambient K concentration was 25 mM. When either lumen or bath K was increased to 25 mM, net Na absorption was reduced. There was spontaneous net K absorption when perfusate and bath K concentration was 5 mM. Analysis of transepithelial K transfer after imposition of chemical gradients demonstrated rectification in the absorptive direction. Absorption of K by this segment provides a means to maintain high medullary interstitial concentration. Accumulation of K in the outer medulla, by reducing NaCl absorption, would increase volume flow through the loop of Henle and increase Na and water delivery to the distal nephron. K recycling thus might provide optimum conditions for K secretion by the distal nephron.
J B Stokes
Title and authors | Publication | Year |
---|---|---|
Distinct cell types along thick ascending limb express pathways for monovalent and divalent cation transport
Demirci H, Bahena-Lopez J, Smorodchenko A, Su XT, Nelson J, Yang CL, Curry J, Duan XP, Wang WH, Sharkovska Y, Liu R, Yilmaz DE, Quintanova C, Emberly K, Emery B, Himmerkus N, Bleich M, Ellison DH, Bachmann S |
bioRxiv | 2025 |
Potassium-Switch Signaling Pathway Dictates Acute Blood Pressure Response to Dietary Potassium.
Welling PA, Little R, Al-Qusairi L, Delpire E, Ellison DH, Fenton RA, Grimm PR |
2024 | |
Going with the flow: New insights regarding flow induced K+ secretion in the distal nephron
Lasaad S, Nickerson AJ, Crambert G, Satlin LM, Kleyman TR |
Physiological Reports | 2024 |
Ubiquitination of NKCC2 by the cullin-RING E3 ubiquitin ligase family in the thick ascending limb of the loop of Henle.
Ares GR |
American journal of physiology. Renal physiology | 2023 |
High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein AM, Palmer LG |
AJP Renal Physiology | 2023 |
Directing two-way traffic in the kidney: A tale of two ions.
Palmer LG |
The Journal of General Physiology | 2022 |
A mathematical model of the rat kidney. IV. Whole kidney response to hyperkalemia.
Weinstein AM |
American journal of physiology. Renal physiology | 2022 |
Potassium Homeostasis and WNK Kinases in the Regulation of the Sodium-Chloride Cotransporter: Hyperaldosteronism and Its Metabolic Consequences
Johnston JG, Wingo CS |
2022 | |
Regulation of the Renal NaCl Cotransporter and Its Role in Potassium Homeostasis
EJ Hoorn, M Gritter, CA Cuevas, RA Fenton |
Physiological reviews | 2020 |
Dietary potassium and the kidney: lifesaving physiology
KY Wei, M Gritter, L Vogt, MH de Borst, JI Rotmans, EJ Hoorn |
Clinical Kidney Journal | 2020 |
Kidney Is Essential for Blood Pressure Modulation by Dietary Potassium
XT Su, CL Yang, DH Ellison |
Current Cardiology Reports | 2020 |
Furosemide reduces BK-αβ4-mediated K + secretion in mice on an alkaline high-K + diet
B Wang, J Wang-France, H Li, SC Sansom |
American journal of physiology. Renal physiology | 2019 |
Intracellular chloride: a regulator of transepithelial transport in the distal nephron
AR Rodan |
Current Opinion in Nephrology and Hypertension | 2019 |
Ion Channels and Transporters in Diabetic Kidney Disease
Spires D, Manis AD, Staruschenko A |
Current topics in membranes | 2019 |
Axial and cellular heterogeneity in electrolyte transport pathways along the thick ascending limb
H Dimke, J Schnermann |
Acta physiologica (Oxford, England) | 2018 |
WNK-SPAK/OSR1 signaling: lessons learned from an insect renal epithelium
AR Rodan |
American journal of physiology. Renal physiology | 2018 |
Renal potassium handling in rats with subtotal nephrectomy: modeling and analysis
AT Layton, A Edwards, V Vallon |
American journal of physiology. Renal physiology | 2018 |
Regulation of renal Na transporters in response to dietary K
L Yang, S Xu, X Guo, S Uchida, AM Weinstein, T Wang, LG Palmer |
American journal of physiology. Renal physiology | 2018 |
Net K + secretion in the thick ascending limb of mice on a low-Na, high-K diet
B Wang, D Wen, H Li, J Wang-France, SC Sansom |
Kidney International | 2017 |
Loop diuretics are K + -sparing in the presence of a low-Na + , high-K + diet
N Boiko, J Berman, E Mironova, JD Stockand |
Kidney International | 2017 |
New options for the management of chronic hyperkalemia
L Fried, CP Kovesdy, BF Palmer |
Kidney International Supplements | 2017 |
Net K + secretion in the thick ascending limb of mice on a low-Na, high-K diet
B Wang, D Wen, H Li, J Wang-France, SC Sansom |
Kidney International | 2017 |
Renal potassium physiology: integration of the renal response to dietary potassium depletion
KS Kamel, M Schreiber, ML Halperin |
Kidney International | 2017 |
Potassium Homeostasis in Health and Disease: A Scientific Workshop Cosponsored by the National Kidney Foundation and the American Society of Hypertension
CP Kovesdy, LJ Appel, ME Grams, L Gutekunst, PA McCullough, BF Palmer, B Pitt, DA Sica, RR Townsend |
American journal of kidney diseases : the official journal of the National Kidney Foundation | 2017 |
Potassium homeostasis in health and disease: A scientific workshop cosponsored by the National Kidney Foundation and the American Society of Hypertension
CP Kovesdy, LJ Appel, ME Grams, L Gutekunst, PA McCullough, BF Palmer, B Pitt, DA Sica, RR Townsend |
Journal of the American Society of Hypertension : JASH | 2017 |
The renal response to potassium stress: integrating past with present
CR Boyd-Shiwarski, AR Subramanya |
Current Opinion in Nephrology and Hypertension | 2017 |
A mathematical model of the rat kidney: K + -induced natriuresis
AM Weinstein |
American journal of physiology. Renal physiology | 2017 |
The renal response to potassium stress: integrating past with present.
Boyd-Shiwarski CR, Subramanya AR |
Current Opinion in Nephrology and Hypertension | 2017 |
Roles and Regulation of Renal K Channels
PA Welling |
Annual Review of Physiology | 2016 |
Potassium: friend or foe?
AR Rodan |
Pediatric Nephrology | 2016 |
Maintaining K + balance on the low-Na + , high-K + diet
RJ Cornelius, B Wang, J Wang-France, SC Sansom |
American journal of physiology. Renal physiology | 2016 |
WNK kinases in development and disease
Rodan AR, Jenny A |
Current topics in developmental biology | 2016 |
Achieving the Benefits of a High-Potassium, Paleolithic Diet, Without the Toxicity
BF Palmer, DJ Clegg |
Mayo Clinic Proceedings | 2016 |
Reducing αENaC expression in the kidney connecting tubule induces pseudohypoaldosteronism type 1 symptoms during K + loading
SB Poulsen, J Praetorius, HH Damkier, L Miller, RD Nelson, E Hummler, BM Christensen |
American journal of physiology. Renal physiology | 2016 |
Physiology and pathophysiology of potassium homeostasis
BF Palmer, DJ Clegg |
AJP Advances in Physiology Education | 2016 |
Dietary potassium and the renal control of salt balance and blood pressure
D Penton, J Czogalla, J Loffing |
Pflügers Archiv - European Journal of Physiology | 2015 |
Low Na, High K Diet and the Role of Aldosterone in BK-Mediated K Excretion
RJ Cornelius, D Wen, H Li, Y Yuan, J Wang-France, PC Warner, SC Sansom, U Sen |
PloS one | 2015 |
Integrated Control of Na Transport along the Nephron
LG Palmer, J Schnermann |
Clinical journal of the American Society of Nephrology : CJASN | 2014 |
Interacting influence of diuretics and diet on BK channel-regulated K homeostasis
D Wen, RJ Cornelius, SC Sansom |
Current Opinion in Pharmacology | 2014 |
Thick Ascending Limb of the Loop of Henle
DB Mount |
Clinical journal of the American Society of Nephrology : CJASN | 2014 |
Regulation of Potassium Homeostasis
BF Palmer |
Clinical journal of the American Society of Nephrology : CJASN | 2014 |
Comprehensive Physiology
SN Cheuvront, RW Kenefick |
Comprehensive Physiology | 2014 |
Seldin and Giebisch's The Kidney
CJ Cooper, LD Dworkin, WL Henrich |
Seldin and Giebisch's The Kidney | 2013 |
Rapid dephosphorylation of the renal sodium chloride cotransporter in response to oral potassium intake in mice
MV Sorensen, S Grossmann, M Roesinger, N Gresko, AP Todkar, G Barmettler, U Ziegler, A Odermatt, D Loffing-Cueni, J Loffing |
Kidney International | 2013 |
Regulation of Renal Potassium Secretion: Molecular Mechanisms
PA Welling |
Seminars in Nephrology | 2013 |
Bicarbonate promotes BK-α/β4-mediated K excretion in the renal distal nephron
RJ Cornelius, D Wen, LI Hatcher, SC Sansom |
American journal of physiology. Renal physiology | 2012 |
Brenner and Rector's The Kidney
C Brugnara, KU Eckardt |
Brenner and Rector's The Kidney | 2012 |
Comprehensive Biophysics
AM Weinstein |
Comprehensive Biophysics | 2012 |
Bicarbonate promotes BK-α/β4-mediated K excretion in the renal distal nephron
RJ Cornelius, D Wen, LI Hatcher, SC Sansom |
American journal of physiology. Renal physiology | 2012 |
Role of BK channels in hypertension and potassium secretion :
JD Holtzclaw, PR Grimm, SC Sansom |
Current Opinion in Nephrology & Hypertension | 2011 |
Recent advances in distal tubular potassium handling
AR Rodan, CJ Cheng, CL Huang |
AJP Renal Physiology | 2011 |
Current Topics in Developmental Biology
JE Qualls, PJ Murray |
Cancer and Development | 2011 |
Recent advances in distal tubular potassium handling
AR Rodan, CJ Cheng, CL Huang |
American journal of physiology. Renal physiology | 2011 |
Multigene kinase network, kidney transport, and salt in essential hypertension
PA Welling, YP Chang, E Delpire, JB Wade |
Kidney International | 2010 |
A mathematical model of rat ascending Henle limb. III. Tubular function
AM Weinstein |
American journal of physiology. Renal physiology | 2010 |
A mathematical model of rat ascending Henle limb. I. Cotransporter function
AM Weinstein |
American journal of physiology. Renal physiology | 2010 |
Osmoregulation of ceroid neuronal lipofuscinosis type 3 in the renal medulla
CS Stein, PH Yancey, I Martins, RD Sigmund, JB Stokes, BL Davidson |
American journal of physiology. Cell physiology | 2010 |
A possible catalytic role for NH 4+ in Na + reabsorption across the thick ascending limb
A Edwards |
American journal of physiology. Renal physiology | 2010 |
A mathematical model of rat ascending Henle limb. II. Epithelial function
AM Weinstein, TA Krahn |
American journal of physiology. Renal physiology | 2010 |
Intercalated Cell BK-α/β4 Channels Modulate Sodium and Potassium Handling During Potassium Adaptation
JD Holtzclaw, PR Grimm, SC Sansom |
Journal of the American Society of Nephrology : JASN | 2010 |
A possible catalytic role for NH4+ in Na+ reabsorption across the thick ascending limb
A Edwards |
American journal of physiology. Renal physiology | 2009 |
A mathematical model of rat ascending Henle limb. II. Epithelial function
AM Weinstein, TA Krahn |
American journal of physiology. Renal physiology | 2009 |
Medical Physiology
G Giebisch, E Windhager |
Medical Physiology | 2009 |
K + secretion in the rat kidney: Na + channel-dependent and -independent mechanisms
G Frindt, LG Palmer |
American journal of physiology. Renal physiology | 2009 |
Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidney
G Frindt, A Shah, J Edvinsson, LG Palmer |
American journal of physiology. Renal physiology | 2009 |
Fluid and Electrolytes in Pediatrics
LG Feld, FJ Kaskel |
2009 | |
Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidney
G Frindt, A Shah, J Edvinsson, LG Palmer |
American journal of physiology. Renal physiology | 2008 |
BK channels in the kidney
PR Grimm, SC Sansom |
Current Opinion in Nephrology and Hypertension | 2007 |
BK channels in the kidney:
PR Grimm, SC Sansom |
Current Opinion in Nephrology and Hypertension | 2007 |
Control of potassium excretion: a Paleolithic perspective
ML Halperin, S Cheema-Dhadli, SH Lin, KS Kamel |
Current Opinion in Nephrology and Hypertension | 2006 |
Relationship and Interaction between Sodium and Potassium
RC Morris, O Schmidlin, LA Frassetto, A Sebastian |
Journal of the American College of Nutrition | 2006 |
Requirements for a high rate of potassium excretion in rats consuming a low electrolyte diet: Control of potassium excretion
S Cheema-Dhadli, SH Lin, C Keong-Chong, KS Kamel, ML Halperin |
The Journal of Physiology | 2006 |
Potassium and sodium transport along the loop of Henle: Effects of altered dietary potassium intake
R Unwin, G Capasso, G Giebisch |
Kidney International | 1994 |
Disorders of Potassium Homeostasis: An Approach Based on Pathophysiology
KS Kamel, S Quaggin, A Scheich, ML Halperin |
American Journal of Kidney Diseases | 1994 |
Potassium and anaesthesia
JE Tetzlaff, JF OHara, MT Walsh |
Canadian Journal of Anesthesia/Journal canadien d'anesthésie | 1993 |
Short-term indomethacin administration does not impair excretion of acute potassium load in humans
MV Buren, HJ van Run, HA Koomans |
European Journal of Clinical Investigation | 1992 |
Insulin increases sodium reabsorption in diluting segment in humans: Evidence for indirect mediation through hypokalemia
CE Friedberg, M Buren, JA Bijlsma, HA Koomans |
Kidney International | 1991 |
Chronopharmacology of the New Uricosuric Diuretic S-8666 in Rats
A Fujimura, K Ohashi, A Ebihara |
The Japanese Journal of Pharmacology | 1991 |
Modulation of Na-K-ATPase activity in the mouse medullary thick ascending limb of Henle. Effects of mineralocorticoids and sodium
EB Grossman, SC Hebert |
Journal of Clinical Investigation | 1988 |
Mineralocorticoid activity and the excretion of an oral potassium load in normal man
RJ Hené, HA Koomans, AJ Rabelink, P Boer, EJ Mees |
Kidney International | 1988 |
Trans- and paracellular K+ transport in diluting segment of frog kidney
A Schwab, H Oberleithner |
Pflügers Archiv European Journal of Physiology | 1988 |
Trans- and paracellular K+ transport in diluting segment of frog kidney
A Schwab, H Oberleithner |
Pflügers Archiv - European Journal of Physiology | 1988 |
The medullary thick limb: Function and modulation of the single–effect multiplier
SC Hebert, WB Reeves, DA Molony, TE Andreoli |
Kidney International | 1987 |
Potassium recycling
RL Jamison |
Kidney International | 1987 |
Physiological control of the urinary concentrating mechanism by peptide hormones
C Rouffignac, JM Elalouf, N Roinel |
Kidney International | 1987 |
Cycles and separations: The histotopography of the urinary concentrating process
KV Lemley, W Kriz |
Kidney International | 1987 |
The cortical thick ascending limb and early distal convoluted tubule in the urinary concentrating mechanism
R Greger, H Velázquez |
Kidney International | 1987 |
Role of K+ conductive pathways in the nephron
R Greger, H Gögelein |
Kidney International | 1987 |
Effects of potassium bicarbonate on distal nephron Na-K-ATPase in adrenalectomized rabbits
L C Garg, N Narang |
Pflügers Archiv - European Journal of Physiology | 1987 |
Effects of potassium bicarbonate on distal nephron Na−K-ATPase in adrenalectomized rabbits
LC Garg, N Narang |
Pflügers Archiv - European Journal of Physiology | 1987 |
Effect of potassium adaptation on the distribution of potassium, sodium and chloride across the apical membrane of renal tubular cells
FX Beck, A Dörge, R Rick, M Schramm, K Thurau |
Pflügers Archiv - European Journal of Physiology | 1987 |
Membrane Transport Processes in Organized Systems
TE Andreoli, JF Hoffman, DD Fanestil, SG Schultz |
1987 | |
Renal adaptation to potassium in the adrenalectomized rabbit. Role of distal tubular sodium-potassium adenosine triphosphatase
LC Garg, N Narang |
Journal of Clinical Investigation | 1985 |
Effect of acute potassium load on reabsorption in Henle's loop in chronic renal failure in the rat
CL Milanes, RL Jamison |
Kidney International | 1985 |
Biochemical Actions of Hormones
D Marver |
Biochemical Actions of Hormones | 1985 |
cAMP increases the basolateral Cl?-conductance in the isolated perfused medullary thick ascending limb of Henle's loop of the mouse
E Schlatter, R Greger |
Pflügers Archiv - European Journal of Physiology | 1985 |
Internephron heterogeneity for carbonic anhydrase-independent bicarbonate reabsorption in the rat
JP Frommer, ME Laski, DE Wesson, NA Kurtzman |
Journal of Clinical Investigation | 1984 |
The maintenance of metabolic alkalosis: Factors which decrease bicarbonate excretion
S Sabatini, NA Kurtzman |
Kidney International | 1984 |
Current Topics in Membranes and Transport
J Gliemann, WD Rees |
Current Topics in Membranes and Transport | 1983 |
Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney: A model for secondary active chloride transport
R Greger, E Schlatter |
Pflügers Archiv - European Journal of Physiology | 1983 |
Evidence for electroneutral sodium chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney
R Greger, E Schlatter, F Lang |
Pflügers Archiv - European Journal of Physiology | 1983 |
Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney.
Greger R, Schlatter E |
1983 | |
Physiology of Membrane Disorders
TE Andreoli, JF Hoffman, DD Fanestil |
1978 |