Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.
J Grayson, … , I R Koski, R M Blaese
J Grayson, … , I R Koski, R M Blaese
Published December 1, 1981
Citation Information: J Clin Invest. 1981;68(6):1539-1547. https://doi.org/10.1172/JCI110408.
View: Text | PDF
Research Article

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.

  • Text
  • PDF
Abstract

The direct effects of steroid hormones on the production of immunoglobulins and DNA synthesis by human T and B lymphocytes was evaluated in cultures of peripheral blood mononuclear cells. As detected by a reverse hemolytic plaque assay, the addition of 0.1 mM to 10 nM hydrocortisone to lymphocytes in culture in the absence of other stimulants or mitogens, resulted in the dramatic induction of immunoglobulin production with responses comparable to those seen in similar cultures stimulated with pokeweed mitogen. Steroid-stimulated immunoglobulin production was first seen after 48 h and peaked at 8-10 d of culture. The production of IgG, IgA, and IgM was induced following incubation with steroid. Glucocorticoids, but not estrogens or androgens, were capable of mediating this effect, and only compounds with affinity for the glucocorticoid receptor were active. The induction of immunoglobulin production was dependent on both T cells and monocytes; cultures depleted of either cell type did not produce immunoglobulin when stimulated with glucocorticoid hormones. Proliferation of B cells or T cells could not be detected by [3H]thymidine incorporation or total cell recovery from steroid-stimulated cultures, even though such cultures demonstrated marked increases in immunoglobulin production. The mechanism responsible for this functional maturation of B cells to become high rate immunoglobulin producing cells is as yet undefined, although it appears to involve more than merely steroid mediated inactivation of suppressor T cells.

Authors

J Grayson, N J Dooley, I R Koski, R M Blaese

×

Total citations by year

Year: 2021 2020 2019 2016 2015 2014 2013 2011 2009 2008 2006 2005 2004 2003 2001 2000 1999 1998 1995 1994 1993 1992 1991 1990 1989 1988 1987 1986 1985 1984 1983 1982 Total
Citations: 2 2 1 3 1 2 1 1 2 2 1 1 1 1 2 3 1 1 2 5 1 3 6 1 1 7 3 4 7 8 4 2 82
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (82)

Title and authors Publication Year
Recommendations and Guidance for Steroid Injection Therapy and COVID-19 Vaccine Administration from the American Society of Pain and Neuroscience (ASPN)
K Chakravarthy, N Strand, A Frosch, D Sayed, LR Narra, R Chaturvedi, PK Grewal, J Pope, ME Schatman, T Deer
Journal of pain research 2021
Effects of Vitamin D and Dexamethasone on Lymphocyte Proportions and Their Associations With Serum Concentrations of 25-Hydroxyvitamin D3In Vitro in Patients With Multiple Sclerosis or Neuromyelitis Optica Spectrum Disorder
EB Cho, JH Shin, S Kwon, J Kim, JM Seok, BJ Kim, JH Min
Frontiers in immunology 2021
Reappraisal of Oral Steroid Therapy for Myasthenia Gravis
T Imai, S Suzuki, Y Nagane, A Uzawa, H Murai, K Utsugisawa
Frontiers in neurology 2020
Control of immunity by glucocorticoids in health and disease
A Shimba, K Ikuta
Seminars in Immunopathology 2020
Regulatory and Effector B Cells: A New Path Toward Biomarkers and Therapeutic Targets to Improve Transplant Outcomes?
A Cherukuri, Q Ding, A Sharma, K Mohib, DM Rothstein
Clinics in Laboratory Medicine 2019
Non-invasive quantification of immunoglobulin A in chimpanzees ( Pan troglodytes schweinfurthii ) at Gombe National Park, Tanzania: Immunoglobulin A in Chimpanzees at Gombe
EL Lantz, EV Lonsdorf, MR Heintz, CM Murray, I Lipende, DA Travis, RM Santymire
American Journal of Primatology 2016
Effects of Corticosterone on Immune Functions of Cultured Rat Splenic Lymphocytes Exposed to Aluminum Trichloride
X Yang, F Xu, C Zhuang, C Bai, W Huang, M Song, Y Han, Y Li
Biological Trace Element Research 2016
Exposure to Corticosterone Affects Host Resistance, but Not Tolerance, to an Emerging Fungal Pathogen
J Murone, JA DeMarchi, MD Venesky, MC Fisher
PloS one 2016
Estrogen receptor signal in regulation of B cell activation during diverse immune responses
J Asaba, M Bandyopadhyay, M Kindy, S Dasgupta
The International Journal of Biochemistry & Cell Biology 2015
To B or Not to B? Glucocorticoid Impact on B Lymphocyte Fate and Function
WJ Kovacs
Endocrinology 2014
Glucocorticoids regulate natural killer cell function epigenetically
JL Eddy, K Krukowski, L Janusek, HL Mathews
Cellular Immunology 2014
Glucocorticoid inhibition of activation-induced cytidine deaminase expression in human B lymphocytes
AL Benko, NJ Olsen, WJ Kovacs
Molecular and Cellular Endocrinology 2013
Advances in Immunology
E Pinaud, M Marquet, R Fiancette, S Péron, C Vincent-Fabert, Y Denizot, M Cogné
Advances in Immunology Volume 110 2011
Enhancing versus suppressive effects of stress on immune function: implications for immunoprotection and immunopathology
FS Dhabhar
Neuroimmunomodulation 2009
Middleton's Allergy: Principles and Practice
RP Schleimer
Middleton's Allergy: Principles and Practice 2009
Psychoneuroimmunology
F Tausk, I Elenkov, J Moynihan
Dermatologic Therapy 2008
Expression of bone specific alkaline phosphatase on human B cells
A Hossain, LK Jung
Cellular Immunology 2008
Systemic Lupus Erythematosus
M Petri
JCR: Journal of Clinical Rheumatology 2006
Methotrexate therapy of oral corticosteroid-dependent asthmatics reduces serum immunoglobulins: correlation with clinical response to therapy
CJ Corrigan, RJ Shiner, BH Shakur, PW Ind
Clinical & Experimental Allergy 2005
Immunoendocrinology in Health and Disease
N Martens, E Hooghe-Peters, R Hooghe
Immunoendocrinology in Health and Disease 2004
Stress, Leukocyte Trafficking, and the Augmentation of Skin Immune Function
FS Dhabhar
Annals of the New York Academy of Sciences 2003
Medroxyprogesterone acetate enhances in vivo and in vitro antibody production
M Vermeulen, P Pazos, C Lanari, A Molinolo, R Gamberale, JR Geffner, M Giordano
Immunology 2001
The process of aging changes the interplay of the immune, endocrine and nervous systems
RH Straub, M Cutolo, B Zietz, J Schölmerich
Mechanisms of Ageing and Development 2001
Glucocorticoids enhance IgE synthesis. Are we heading towards new paradigms?
SS Salvi, KS Babu, ST Holgate
Clinical & Experimental Allergy 2000
Alterations in Serum Protein Levels in Patients with Cushing’s Syndrome before and after Successful Treatment
P Putignano, GA Kaltsas, M Korbonits, PJ Jenkins, JP Monson, GM Besser, AB Grossman
The Journal of clinical endocrinology and metabolism 2000
How Do Glucocorticoids Influence Stress Responses? Integrating Permissive, Suppressive, Stimulatory, and Preparative Actions*
RM Sapolsky, LM Romero, AU Munck
Endocrine reviews 2000
CELLULAR EFFECTS OF GLUCOCORTICOIDS
CJ Corrigan
Immunology and Allergy Clinics of North America 1999
Sympathectomy-Induced Immune Changes Are Not Abrogated by the Glucocorticoid Receptor Blocker RU-486
B Kruszewska, DL Felten, SY Stevens, JA Moynihan
Brain, Behavior, and Immunity 1998
Immunoglobulin production in vitro in major depression: A pilot study on the modulating action of endogenous cortisol
FW Kok, CJ Heijnen, JA Bruijn, HG Westenberg, JM van Ree
Biological Psychiatry 1995
Effect of bursal anti-steroidogenic peptide (BASP) on cortisol biosynthesis in ACTH-stimulated canine adrenocortical carcinoma cells in vitro
J Byrd
Veterinary Immunology and Immunopathology 1995
Synthetic glucocorticoids potentiate IgE synthesis
HG Nüsslein, G Weber, JR Kalden
Allergy 1994
The effect of the humoral immune system-derived bursal anti-steroidogenic peptide (BASP) on corticosteroid biosynthesis in avian, porcine and canine adrenal cortical cells
JA Byrd, CE Dean, BM Hargis
Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 1994
Permissive role of glucocorticoids on interleukin-1 activation of the hypothalamic serotonergic system
C Gemma, AD Luigi, MG Simoni
Brain Research 1994
In vitro and in vivo effect of glucocorticoids on IgE and IgG subclass secretion
F Klebl, G Weber, JR Kalden, HG Nusslein
Clinical & Experimental Allergy 1994
Immunity and the Endocrine System
DS Greco, LM Harpold
Veterinary Clinics of North America Small Animal Practice 1994
Development of specific antibody andin vivo response to antigen in different rat strains: Effect of dexamethasone and importance of endogenous corticosteroids
SH Peers, GS Duncan, RJ Flower
Agents and Actions 1993
Increased sensitivity of glioblastoma cells to interleukin 1 after long-term incubation with dexamethasone
PE gottschall, I Tatsuno, A Arimura
Molecular and Cellular Neuroscience 1992
Lymphoblastosis inhibition and plaque-forming cell response of several anti-inflammatory steroids in mice
HP Choi, K Kim, HP Kim
Archives of Pharmacal Research 1992
Interleukin–1 in the Brain
PE Gottschall, G Komaki, A Arimura
Interleukin–1 in the Brain 1992
Glucocorticoids increase the synthesis of immunoglobulin E by interleukin 4-stimulated human lymphocytes
CY Wu, M Sarfati, C Heusser, S Fournier, M Rubio-Trujillo, R Peleman, G Delespesse
Journal of Clinical Investigation 1991
Regulation of transforming growth factor-beta 1 gene expression by glucocorticoids in normal human T lymphocytes
O AyanlarBatuman, AP Ferrero, A Diaz, SA Jimenez
Journal of Clinical Investigation 1991
The Influence of Corticosteroids on Host Defense Mechanisms
LA Cohn
Journal of Veterinary Internal Medicine 1991
Influence of cytokines and cellular interactions on the glucocorticoid-induced Ig (E, G, A, M) synthesis of peripheral blood mononuclear cells
A Fischer, W König
Immunology 1991
Cortisol and immunity
WM Jefferies
Medical Hypotheses 1991
Glucocorticoid upregulation of interleukin 1 receptor expression in a glioblastoma cell line
PE Gottschall, K Koves, K Mizuno, I Tatsuno, A Arimura
American journal of physiology. Endocrinology and metabolism 1991
Lipopolysaccharide and dexamethasone induce mouse mammary tumor proviral gene expression and differentiation in B lymphocytes through distinct regulatory pathways
LB King, RB Corley
Molecular and cellular biology 1990
Direct Effects of Glucocorticosteroids on Epidermal Langerhans Cells
M Furue, SI Katz
Journal of Investigative Dermatology 1989
Differential mechanism for differentiation into immunoglobulin-secreting cells in human resting B lymphocyte subsets isolated on the basis of cell density
N Suzuki, Y Ueda, T Sakane
Journal of Clinical Investigation 1988
Interleukin 1 stimulates its own receptor expression on human fibroblasts through the endogenous production of prostaglandin(s)
T Akahoshi, JJ Oppenheim, K Matsushima
Journal of Clinical Investigation 1988
Induction of high-affinity interleukin 1 receptor on human peripheral blood lymphocytes by glucocorticoid hormones
T Akahoshi, JJ Oppenheim, K Matsushima
Journal of Experimental Medicine 1988
Mechanism of action of glucocorticoid-induced immunoglobulin production: II. Requirement for fetal calf serum
S Sierakowski, JS Goodwin
Clinical & Experimental Immunology 1988
Glucocorticosteroid-induced immunoglobulin production requires intimate contact between B cells and monocytes
FM Orson, CA Auzenne
Cellular Immunology 1988
Stimulation of IgG production by glucocorticoids in human myeloma lymphoblasts
DS Manzer, BA Littlefield
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1988
The Pharmacology of Lymphocytes
MA Bray, J Morley
1988
B cell differentiation and interleukin 2 (IL 2): corticosteroids interact with monocytes to enhance the effect of IL 2
D Emilie, S Karray, MC Crevon, A Vazquez, P Galanaud
European Journal of Immunology 1987
Immunology of the Neonate
GR Burgio, LÅ Hanson, AG Ugazio
1987
Mechanisms of Lymphocyte Activation and Immune Regulation
S Gupta, WE Paul, AS Fauci
1987
Effects of immunosuppressive therapy with prednisolone on B and T lymphocyte function in patients with chronic type B hepatitis
RG Hanson, MG Peters, JH Hoofnagle
Hepatology 1986
Lymphocyte function in human bone marrow
T Hibi, HM Dosch
Cellular Immunology 1986
Independent regulation of B-cell inducing factor and IL-2 production by T lymphocytes, and direct and indirect promotion of immunoglobulin secretion by glucocorticosteroid
G Jeong, I Nakoinz, P Ralph
Cellular Immunology 1986
Enkephalins and Endorphins
NP Plotnikoff, RE Faith, AJ Murgo, RA Good
1986
Effects of in vitro corticosteroids on B cell activation, proliferation, and differentiation
TR Cupps, TL Gerrard, RJ Falkoff, G Whalen, AS Fauci
Journal of Clinical Investigation 1985
Transferrin receptor induction is required for human B-lymphocyte activation but not for immunoglobulin secretion
LM Neckers, G Yenokida, JB Trepel, E Lipford, S James
Journal of Cellular Biochemistry 1985
Cellular distribution of a human I-A-like (DS/DC) antigen on normal and neoplastic lymphoid cells
GE Marti, LM Nadler, TM Chused, G Tosato, RM Blaese, TJ Kindt
Human Immunology 1985
Actions des glucocorticostèroïdes sur les fonctions lymphocytaires
F Frey
Médecine et Maladies Infectieuses 1985
Differentiation and maturation of human B lymphocytes: A review
H Zola
Pathology 1985
Immunosuppressive Agents and Plasmapheresis in Immunological Disorders
GC Tsokos, JE Balow
Immunopharmacology and Immunotoxicology 1985
Partial in vivo response to corticosteroid treatment in common variable immune deficiency
V Wahn, U Göbel, H Grosse-Wilde, H Rosin, C Carls
Infection 1985
Detection of Anti-HBc IgM Following Prednisone Treatment in Patients with Chronic Active Hepatitis B Virus Infection
MJ Nowicki, MJ Tong, PV Nair, D Stevenson
Hepatology 1984
Specificity of Antigen Induced Helper Factor for Antibody SynthesisIn Vitroand its Relation to Lymphocytes Interaction
N Khansari, HH Fudenberg
Immunological Investigations 1984
Role of Autorosette Forming Cells in Antibody Synthesis in vitro: Suppressive Activity of ARFC in Humoral Immune Response
N Khansari, M Petrini, F Ambrogii, P Goldschmidt-Clermont, HH Fudenberg
Immunobiology 1984
The role of PNP enzyme in autologous rosette-forming cells
P Goldschmidt-Clermont, M Petrini, N Khansari, HH Fudenberg
Cellular Immunology 1984
Immunological dysfunction in Alzheimer's disease
N Khansari, HD Whitten, YK Chou, HH Fudenberg
Journal of Neuroimmunology 1984
Systemic lupus erythematosus with paraproteinemia
L Rubin, MB Urowitz, W Pruzanski
Arthritis & Rheumatism 1984
Specificity of Antigen Induced Helper Factor for Antibody Synthesis In Vitro and its Relation to Lymphocytes Interaction
N Khansari, HH Fudenberg
Immunological Investigations 1984
Phencyclidine-Induced Immunodepression
N Khansari, HD Whitten, HH Fudenberg
Science 1984
In vitro synthesis of IgG and IgM in patients with HBsAg-positive and HBsAg-negative chronic active hepatitis
G Triolo, S Nardiello, E Sagnelli, F Schaffner, S Vernace, F Paronetto
Liver 1983
T cell-replacing factor for glucocorticosteroid-induced immunoglobulin production. A unique steroid-dependent cytokine
FM Orson, J Grayson, S Pike, VD Seau, RM Blaese
Journal of Experimental Medicine 1983
Klebsiella pneumoniae stimulate highly purified human blood B cells to mature into plaque forming cells without prior proliferation
WL Gross, A Rucks
Clinical & Experimental Immunology 1983
In Vitro Humoral Immune Response of Human Peripheral Blood Lymphocytes to Tetanus Toxoid Sepharose 4B
N Khansari, HH Fudenberg, E Merler
Immunobiology 1983
Corticosteroid-Mediated Immunoregulation in Man
TR Cupps, AS Fauci
Immunological Reviews 1982
Corticosteroid-Induced Modulation of Immunoglobulin Secretion by Human B Lymphocytes: Potentiation of Background Mitogenic Signals
TR Cupps, LC Edgar, AS Fauci
Immunopharmacology and Immunotoxicology 1982

← Previous 1 2 3 4 Next →

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts