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Citations to this article

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.
J Grayson, … , I R Koski, R M Blaese
J Grayson, … , I R Koski, R M Blaese
Published December 1, 1981
Citation Information: J Clin Invest. 1981;68(6):1539-1547. https://doi.org/10.1172/JCI110408.
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Research Article

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.

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Abstract

The direct effects of steroid hormones on the production of immunoglobulins and DNA synthesis by human T and B lymphocytes was evaluated in cultures of peripheral blood mononuclear cells. As detected by a reverse hemolytic plaque assay, the addition of 0.1 mM to 10 nM hydrocortisone to lymphocytes in culture in the absence of other stimulants or mitogens, resulted in the dramatic induction of immunoglobulin production with responses comparable to those seen in similar cultures stimulated with pokeweed mitogen. Steroid-stimulated immunoglobulin production was first seen after 48 h and peaked at 8-10 d of culture. The production of IgG, IgA, and IgM was induced following incubation with steroid. Glucocorticoids, but not estrogens or androgens, were capable of mediating this effect, and only compounds with affinity for the glucocorticoid receptor were active. The induction of immunoglobulin production was dependent on both T cells and monocytes; cultures depleted of either cell type did not produce immunoglobulin when stimulated with glucocorticoid hormones. Proliferation of B cells or T cells could not be detected by [3H]thymidine incorporation or total cell recovery from steroid-stimulated cultures, even though such cultures demonstrated marked increases in immunoglobulin production. The mechanism responsible for this functional maturation of B cells to become high rate immunoglobulin producing cells is as yet undefined, although it appears to involve more than merely steroid mediated inactivation of suppressor T cells.

Authors

J Grayson, N J Dooley, I R Koski, R M Blaese

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Total citations by year

Year: 2021 2020 2019 2016 2015 2014 2013 2011 2009 2008 2006 2005 2004 2003 2001 2000 1999 1998 1995 1994 1993 1992 1991 1990 1989 1988 1987 1986 1985 1984 1983 1982 Total
Citations: 2 2 1 3 1 2 1 1 2 2 1 1 1 1 2 3 1 1 2 5 1 3 6 1 1 7 3 4 7 8 4 2 82
Citation information
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Citations to this article in year 2011 (1)

Title and authors Publication Year
Advances in Immunology
E Pinaud, M Marquet, R Fiancette, S Péron, C Vincent-Fabert, Y Denizot, M Cogné
Advances in Immunology Volume 110 2011

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